The antigenic relationships between seven members of the tick-borne encephalitis complex of flaviviruses (group B arboviruses) were examined by raising a library of 16 monoclonal antibodies against one of them and examining their biological and antigenic properties. These clones reacted with only one of two intracellular, virus-specific polypeptides. One polypeptide [mol. wt. 58 × 103 (58K)] is related to the major envelope protein E, but the identity of the other is at present unknown, even though it is a major immunogen in experimental infections and vaccinations. Only those clones specific for the 58K polypeptide contain either neutralizing or haemagglutinin-inhibiting activity, but these epitopes are not identical. In general, epitopes on the 51K polypeptide were more heavily conserved than those on the 58K polypeptide, although both conserved and variant epitopes were found on both polypeptides. One epitope was present on the 51K polypeptide which was conserved on all seven isolates studied and another epitope on the same polypeptide was specific for all the western isolates including one isolate of louping-ill virus. Using the monoclonal antibodies raised in the study, it was shown that louping-ill virus was closely related antigenically to isolates of the Western subtype of tick-borne encephalitis virus.
ChanasA. C.,
GouldE. A.,
CleggJ. C. S.,
VarmaM. G. R.1982; Monoclonal antibodies to Sindbis virus glycoprotein El can neutralize, enhance infectivity and independently inhibit haemagglutination or haemolysis. Journal of General Virology 58:37–46
HambletonP.,
StephensonJ. R.,
BaskervilleA.,
WiblinC. N.1983; Tick-borne encephalitis in primates: pathology, immune response and efficacy of vaccination. Infection and Immunity 40:995–1003
HeinzF. X.,
TumaW.,
KunzC. H.1981; Antigenic and immunogenic properties of defined physical forms of tick-borne encephalitis virus structural proteins. Infection and Immunity 33:250–257
HeinzF. X.,
BergerO. M.,
KnappW.,
KunzC.1982; Monoclonal antibodies to the structural glycoprotein of tick-borne encephalitis virus. Infection and Immunity 37:869–874
KöhlerG.,
HoweS. C.,
MilsteinC.1976; Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines. European Journal of Immunology 6:292–295
PosseeR. D.,
DimmockN. J.1981; Neutralization of influenza virus by antibody: attachment, uptake and uncoating of neutralized virus in chick embryo cells. In Genetic Variation Among Influenza Viruses pp 473–480 ICN-UCLA Symposium XXI Edited by
NayakD. P.,
FoxC. F.
London: Academic Press;
PosseeR. D.,
SchildG. C.,
DimmockN. J.1982; Studies on the mechanism of neutralization of influenza virus by antibody: evidence that neutralizing antibody (anti-haemagglutinin) inactivates influenza virus in vivo by inhibiting virion transcriptase activity. Journal of General Virology 58:373–386
ReidH. W.,
DuncanJ. S.,
PhillipsJ. D. P.,
MossR.,
WatsonA.1978; Studies on Louping 111 virus (flavivirus group) in wild red grouse (Lagopus lagopus scoticus). Journal of Hygiene 81:321–330
SchlesingerJ. J.,
BrandrissM. W.,
MonathT. P.1983; Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, haemagglutination inhibition and immune precipitation of the virus envelope protein. Virology 125:8–17
StephensonJ. R.,
HayA. J.,
SkehelJ. J.1977; Characterization of virus-specific messenger RNAs from avian fibroblasts infected with fowl plague virus. Journal of General Virology 36:237–248
StephensonJ. R.,
ter MeulenV.,
KiesslingW.1980; Search for canine distemper virus antibodies in multiple sclerosis: a detailed virological evaluation. Lancet ii:772–775