1887

Abstract

SUMMARY

The selective and potent anti-herpesvirus drug, ()-5-(2-bromovinyl)-2′-deoxyuridine (BVdU), has been examined for its inhibitory effects on several parameters of Epstein—Barr virus (EBV) infection in the lymphoblastoid cell lines Raji, P3HR-1, B-95-8 and P3 hybrid cells (a human embryo oropharyngeal cell line fused with a nasopharyngeal carcinoma cell line). At a dosage of 0.03 to 0.1 m, BVdU caused a marked inhibition of (i) spontaneous viral capsid antigen (VCA) expression in B-95-8 and P3 hybrid cells, (ii) VCA expression and DNA synthesis in B-95-8 cells induced with croton oil and n-butyrate, (iii) early antigen (EA) expression and DNA synthesis in Raji cells superinfected with EBV, and (iv) VCA expression and DNA synthesis in B-95-8 cells superinfected with EBV. In its inhibitory effects on these various parameters of EBV infection, BVdU appears to be comparable to acyclovir [9-(2-hydroxyethoxymethyl)guanine], another selective anti-herpesvirus drug which has been previously recognized as an effective inhibitor of EBV replication.

Keyword(s): antigens , BVdU , DNA synthesis and EBV
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/content/journal/jgv/10.1099/0022-1317-65-1-37
1984-01-01
2022-01-22
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References

  1. Allaudeen H. S., Kozarich J. W., Bertino J. R., De Clercq E. 1981; On the mechanism of selective inhibition of herpesvirus replication by (E)-5-(2-bromovinyl)-2′-deoxyuridine. Proceedings of the National Academy of Sciences, U. S. A 78:2698–2702
    [Google Scholar]
  2. Allaudeen H. S., Chen M. S., Lee J. J., De Clercq E., Prusoff W. H. 1982a; Incorporation of E-5-(2-halovinyl)-2′-deoxyuridines into deoxyribonucleic acids of herpes simplex virus type 1 -infected cells. Journal of Biological Chemistry 257:603–606
    [Google Scholar]
  3. Allaudeen H. S., Descamps J., Sehgal R. K. 1982b; Mode of action of acyclovir triphosphate on herpesviral and cellular DNA polymerases. Antiviral Research 2:123–133
    [Google Scholar]
  4. Cheng Y.-C., Dutschman G., De Clercq E., Jones A. S., Rahim S. G., Verhelst G., Walker R. T. 1981; Differential affinities of 5-(2-halogenovinyl)-2′-deoxyuridines for deoxythymidine kinases of various origins. Molecular Pharmacology 20:230–233
    [Google Scholar]
  5. Colby B. M., Shaw J. E., Elion G. B., Pagano J. S. 1980; Effect of acyclovir [9-(2-hydroxyethoxymethyl)-guanine] on Epstein-Barr virus DNA replication. Journal of Virology 34:560–568
    [Google Scholar]
  6. Colby B. M., Furman P. A., Shaw J. E., Elion G. B., Pagano J. S. 1981; Phosphorylation of acyclovir [9-(2-hydroxyethoxymethyl)guanine] in Epstein-Barr virus-infected lymphoblastoid cell lines. Journal of Virology 38:606–611
    [Google Scholar]
  7. Datta A. K., Hood R. E. 1981; Mechanism of inhibition of Epstein-Barr virus replication by phosphonoformic acid. Virology 114:52–59
    [Google Scholar]
  8. De Clercq E., Descamps J., De Somer P., Barr P. J., Jones A. S., Walker R. T. 1979; (E)-5-(2-BromOvinyl)-2′-deoxyuridine: a potent and selective anti-herpes agent. Proceedings of the National Academy of Sciences, U. S. A 76:2947–2951
    [Google Scholar]
  9. De Clercq E., Descamps J., Ogata M., Shigeta S. 1982; In vitro susceptibility of varicella-zoster virus to E-5-(2-bromovinyl)-2′-deoxyuridine and related compounds. Antimicrobial Agents and Chemotherapy 21:33–38
    [Google Scholar]
  10. Descamps J., De Clercq E. 1981; Specific phosphorylation of E-5-(2-iodovinyl)-2′-deoxyuridine by herpes simplex virus-infected cells. Journal of Biological Chemistry 256:5973–5976
    [Google Scholar]
  11. Epstein M. A., Achong B. G., Barr Y. M., Zajac B., Henle G., Henle W. 1966; Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). Journal of the National Cancer Institute 37:547–559
    [Google Scholar]
  12. Fyfe J. A. 1982; Differential phosphorylation of (E-5-(2-bromovinyl)-2′-deoxyuridine monophosphate by thymidylate kinases from herpes simplex viruses types 1 and 2 and varicella zoster virus. Molecular Pharmacology 21:432–437
    [Google Scholar]
  13. Henle W., Henle G., Zajac B. A., Pearson G., Waubke R., Scriba M. 1970; Differential reactivity of human serums with early antigens induced by Epstein-Barr virus. Science 169:188–190
    [Google Scholar]
  14. Hinuma Y., Grace J. T. JR 1967; Cloning of immunoglobulin-producing human leukemic and lymphoma cells in long-term cultures. Proceedings of the Society for Experimental Biology and Medicine 124:107–111
    [Google Scholar]
  15. Honess R. W., O’Hare P., Young D. 1982; Comparison of thymidine kinase activities induced in cells productively infected with herpesvirus saimiri and herpes simplex virus. Journal of General Virology 58:237–249
    [Google Scholar]
  16. Jones A. S., Verhelst G., Walker R. T. 1979; The synthesis of the potent anti-herpes virus agent, E-5-(2-bromovinyl)-2′-deoxyuridine and related compounds. Tetrahedron Letters 45:4415–4418
    [Google Scholar]
  17. Lin J.-C., Smith M. C., Pagano J. S. 1981; Induction of replication of Epstein-Barr virus DNA by 12-0 tetradecanoyl-phorbol-13-acetate. II. Inhibition by retinoic acid and 9-(2-hydroxyethoxymethyl)guanine. Virology 111:294–298
    [Google Scholar]
  18. Liu Y.-X., Zhen Y., Dong W.-P., Gao G.-V. 1979; Detection of EBV specific IgA antibody from patients with nasopharyngeal carcinoma by immunoenzymatic method. Chinese Journal of Oncology 1:8–12
    [Google Scholar]
  19. Mancini W. R., De Clercq E., Prusoff W. H. 1983; The relationship between incorporation of E-5-(2′-bromovinyl)-2′-deoxyuridine into herpes simplex virus type 1 DNA with virus infectivity and DNA integrity. Journal of Biological Chemistry 258:792–795
    [Google Scholar]
  20. Margalith M., Manor D., Usieli V., Goldblum N. 1980; Phosphonoformate inhibits synthesis of Epstein-Barr virus (EBV) capsid antigen and transformation of human cord blood lymphocytes by EBV. Virology 102:226–230
    [Google Scholar]
  21. Miller G., Lipman M. 1973; Comparison of the yield of infectious virus from clones of human and simian lymphoblastoid lines transformed by Epstein-Barr virus. Journal of Experimental Medicine 138:1398–1412
    [Google Scholar]
  22. Nakane P. K., Kawaoi A. 1974; Peroxidase-labeled antibody. A new method of conjugation. Journal of Histochemistry and Cytochemistry 22:1084–1091
    [Google Scholar]
  23. Ooka T., Calender A. 1980; Effects of arabinofuranosylthymine on Epstein-Barr virus replication. Virology 104:219–223
    [Google Scholar]
  24. Reefschlager J., Barwolff D., Engelmann P., Langen P., Rosenthal H. A. 1982; Efficiency and selectivity of (E)-5-(2-bromovinyl)-2′-deoxyuridine and some other 5-substituted 2′-deoxypyrimidine nucleosides as antiherpes agents. Antiviral Research 2:41–52
    [Google Scholar]
  25. Sagi J., Szabolcs A., Szemzo A., Otvos L. 1981; (E-5-(2-Bromovinyl)-2′-deoxyuridine-5′-triphosphate as a DNA polymerase substrate. Nucleic Acids Research 9:6985–6994
    [Google Scholar]
  26. Shaw J. E., Seebeck T., Li J.-L. H., Pagano J. S. 1977; Epstein-Barr virus DNA synthesized in superinfected Raji cells. Virology 77:762–771
    [Google Scholar]
  27. Soike K. F., Gibson S., Gerone P. J. 1981; Inhibition of simian varicella virus infection of African green monkeys by (E)-5-(2-bromovinyl)-2′-deoxyuridine (BVDU). Antiviral Research 1:325–337
    [Google Scholar]
  28. Summers W. C., Klein G. 1976; Inhibition of Epstein-Barr virus DNA synthesis and late gene expression by phosphonoacetic acid. Journal of Virology 18:151–155
    [Google Scholar]
  29. Thorley-Lawson D., Strominger J. L. 1976; Transformation of human lymphocytes by Epstein-Barr virus is inhibited by phosphonoacetic acid. Nature, London 263:332–334
    [Google Scholar]
  30. Wang X., Lescott T., De Clercq E., Kelly D. C. 1983; Baculovirus replication: inhibition of Trichoplusia ni multiple nuclear polyhedrosis virus by [E]-5-(2-bromovinyl)-2′-deoxy uridine. Journal of General Virology 64:1221–1227
    [Google Scholar]
  31. Weinmaster G. A., Misra V., McGuire R., Babiuk L. A., De Clercq E. 1982; Bovid herpesvirus type-1 (infectious bovine rhinotracheitis virus)-induced thymidine kinase. Virology 118:191–201
    [Google Scholar]
  32. Yajima Y., Tanaka A., Nonoyama M. 1976; Inhibition of productive replication of Epstein-Barr virus DNA by phosphonoacetic acid. Virology 71:352–354
    [Google Scholar]
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