1887

Abstract

SUMMARY

The RNA segments of bovine rotavirus (UK Compton strain) were measured by electron microscopy in mixtures containing φX174 double-stranded DNA as an internal reference molecule. The numbers of base pairs per segment were calculated and compared with data obtained by other procedures (comparative RNA gel electrophoresis; co-electrophoresis of cDNAs in denaturing gels; sequencing).

Keyword(s): EM , RNA segments , rotavirus and sizes
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-1-233
1984-01-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/1/JV0650010233.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-1-233&mimeType=html&fmt=ahah

References

  1. Bellamy A. R., Shapiro L., August J. T., Joklik W. K. 1967; Studies on reovirus RNA. I. Characterization of reovirus genome RNA. Journal of Molecular Biology 29:1–17
    [Google Scholar]
  2. Both G. W., Bellamy A. R., Street J. E., Siegman L. J. 1982; A general strategy for cloning double-stranded RNA: nucleotide sequence of the simian-11 rotavirus gene 8. Nucleic Acids Research 10:7075–7088
    [Google Scholar]
  3. Both G. W., Mattick J. S., Bellamy A. R. 1983; Serotype-specific glycoprotein of simian-11 rotavirus: coding assignment and gene sequence. Proceedings of the National Academy of Sciences, U. S. A 80:3091–3095
    [Google Scholar]
  4. Brownlee G. G. 1981; Antigenic variation and structure of influenzavirus. In Expression of Eucaryotic Viral and Cellular Genes pp 129–140 Edited by Petersson R., Kaariainen L., Soderlund H., Oker-Blom H. New York & London: Academic Press;
    [Google Scholar]
  5. Cantor C. R., Schimmel P. R. 1980 Biophysical Chemistry part 1 pp 176–186 San Francisco: W. H. Freeman.;
    [Google Scholar]
  6. Cashdollar L. W., Esparza J., Hudson G. R., Chmelo R., Lee P. W. K., Joklik W. K. 1982; Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene. Proceedings of the National Academy of Sciences, U. S. A 79:7644–7648
    [Google Scholar]
  7. Dunnebacke T. H., Kleinschmidt A. K. 1967; Ribonucleic acid from reovirus as seen in protein monolayers by electron microscopy. Zeitschrift fur Naturforschung 22b:159–164
    [Google Scholar]
  8. Dyall-smith M. L., Ellerman T. C., Hoyne P. A., Holmes I. H., Azad A. A. 1983; Cloning and sequence of UK bovine rotavirus gene segment 7: marked sequence homology with simian rotavirus gene segment 8. Nucleic Acids Research 11:3351–3362
    [Google Scholar]
  9. Espejo R. T., Avendano L. F., Munoz O., Romero P., Eternod J. G., Lopez S., Moncaya J. 1980; Comparison of human rotaviruses isolated in Mexico City and in Santiago, Chile, by electrophoretic migration of their double-stranded ribonucleic acid genome segments. Infection and Immunity 30:342–348
    [Google Scholar]
  10. Flewett T. H., Woode G. N. 1978; The rotaviruses. Archives of Virology 57:1–33
    [Google Scholar]
  11. Follett E. A. C., Desselberger U. 1983; Cocirculation of different rotavirus strains during a local outbreak of infantile gastroenteritis. Monitoring by rapid and sensitive nucleic acid analysis. Journal of Medical Virology 11:39–52
    [Google Scholar]
  12. Imai M., Richardson M. A., Ikegami N., Shatkin A. J., Furuichi Y. 1983; Molecular cloning of double-stranded RNA virus genomes. Proceedings of the National Academy of Sciences, U. S. A 80:373–377
    [Google Scholar]
  13. Kalica A. R., Garon C. F., Wyatt R. G., Mebus C. A., van Kirk D. H., Chanock R. M., Kapikian A. Z. 1976; Differentiation of human and calf reovirus-like agents associated with diarrhea using polyacrylamide gel electrophoresis of RNA. Virology 74:86–92
    [Google Scholar]
  14. Kalica A. R., Sereno M. M., Wyatt R. G., Mebus C. A., Chanock R. M., Kapikian A. Z. 1978; Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology 87:247–255
    [Google Scholar]
  15. Kleinschmidt A. K. 1968; Monolayer techniques in electron microscopy of nucleic acid molecules. Methods in Enzymology 12B:361–377
    [Google Scholar]
  16. Lourenco M. H., Nicolas J. C., Cohen J., Scherrer R., Bricout F. 1981; Study of human rotavirus genome by electrophoresis: attempt of classification among strains isolated in France. Annates de Virologie 132E:161–173
    [Google Scholar]
  17. Mccrae M. A., Mccorquodale J. G. 1982; Molecular biology of rotaviruses. IV. Molecular cloning of the bovine rotavirus genome. Journal of Virology 44:1076–1079
    [Google Scholar]
  18. McNulty M. S. 1978; Rotaviruses. Journal of Genera! Virology 40:1–18
    [Google Scholar]
  19. Martin S. A., Zweerink H. J. 1972; Isolation and characterization of two types of bluetongue virus particles. Virology 50:495–506
    [Google Scholar]
  20. Newman J. F. E., Brown F., Bridger J. C., Woode G. N. 1975; Characterization of a rotavirus. Nature, London 258:631–633
    [Google Scholar]
  21. Pereira H. G., Azeredo R. S., Leite J. P. G., Candeias J. A. N., Racz M. L., Linhares A. C., Gabbay G. B., Trabulsi J. R. 1983; Electrophoretic study of the human rotaviruses from Rio de Janeiro, Sao Paulo and Para, Brazil. Journal of Hygiene 90:117–125
    [Google Scholar]
  22. Priess H., Koller B., Hess B., Delius H. 1980; Electron microscopic mapping and sequence analysis of the terminator for the early message of Escherichia coli phage T7. Molecular and General Genetics 178:27–34
    [Google Scholar]
  23. Rodger S. M., Holmes I. H. 1979; Comparison of the genomes of simian, bovine and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. Journal of Virology 30:839–846
    [Google Scholar]
  24. Rodger S. M., Schnagl R. D., Holmes I. H. 1975; Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin. Journal of Virology 16:1229–1235
    [Google Scholar]
  25. Sanger F., Coulson A. R., Friedmann T., Air G. M., Barrell B. G., Brown N. L., Fiddes J. C., Hutchinson C. A., Slocombe P. M., Smith M. 1978; The nucleotide sequence of bacteriophage ϕX174. Journal of Molecular Biology 125:225–246
    [Google Scholar]
  26. Shatkin A. J., Sipe J. D., Loh P. 1968; Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. Journal of Virology 2:986–991
    [Google Scholar]
  27. Todd D., McNulty M. S. 1976; Characterization of pig rotavirus RNA. Journal of General Virology 33:147–150
    [Google Scholar]
  28. Todd D., McNulty M. S. 1977; Biochemical studies on a reovirus-like agent (rotavirus) from lambs. Journal of Virology 21:1215–1218
    [Google Scholar]
  29. Todd D., McNulty M. S., Allan G. M. 1980; Polyacrylamide gel electrophoresis of avian rotavirus RNA. Archives of Virology 63:87–97
    [Google Scholar]
  30. Vasquez C., Kleinschmidt A. K. 1968; Electron microscopy of RNA strands released from individual reovirus particles. Journal of Molecular Biology 34:137–147
    [Google Scholar]
  31. Verly E., Cohen J. 1977; Demonstration of size variation of RNA segments between different isolates of calf rotavirus. Journal of General Virology 35:583–586
    [Google Scholar]
  32. Whitton J. L., Hundley F., O’donnell B., Desselberger U. 1983; Silver staining of nucleic acids. Application in virus research and in diagnostic virology. Journal of Virological Methods (in press)
    [Google Scholar]
  33. Wood H. A. 1973; Viruses with double-stranded RNA genomes. Journal of General Virology 20:61–85
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-1-233
Loading
/content/journal/jgv/10.1099/0022-1317-65-1-233
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error