RT Journal Article SR Electronic(1) A1 Williams, Marshall V.YR 1984 T1 Demonstration of a Herpes Simplex Virus Type 2-induced Deoxyuridine Triphosphate Nucleotidohydrolase in Infected KB Cells and in Biochemically Transformed HeLa Cells JF Journal of General Virology, VO 65 IS 1 SP 209 OP 213 DO https://doi.org/10.1099/0022-1317-65-1-209 PB Microbiology Society, SN 1465-2099, AB SUMMARY The deoxyuridine triphosphate nucleotidohydrolase (dUTPase) activity which is induced in KB cells infected with herpes simplex virus type 2 (HSV-2) and expressed in biochemically transformed HeLa cells was separated from the host dUTPase activity using either gel filtration or chromatofocusing chromatography. The HSV-2-induced dUTPase differed from the host dUTPase activity in its molecular weight (53000 versus 46000) and isoelectric point (pI 5.9 versus 6.4). However, it was similar to the host enzyme in that only dUTP served as a substrate for the enzyme., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-1-209