1887

Abstract

SUMMARY

Three newly established monoclonal hybridoma antibodies to the haemagglutinin molecule of HVJ, designated A7, B3 and F11, recognize operationally non-overlapping antigenic determinants and have neutralizing activity. Using these antibodies, the frequencies of occurrence of neutralization-resistant antigenic variants were analysed in virus populations released from four cell lines persistently infected with HVJ, namely GM2-HVJ, LLCMK2-HVJ, Vero-HVJ and GEsl-HVJ at various passage stages. Antigenic variants were selected from culture fluids of these HVJ carrier cells at a total frequency of 10, 10 and 10 by monoclonal antibodies A7, B3 and F11, respectively. These values were considerably higher than those of 10 to 10 detected in a stock preparation of wild-type virus with these antibodies. All the variant viruses isolated as above were negative in neutralization, haemagglutination inhibition and immunofluorescent staining tests with each monoclonal antibody used for their isolation, but were positive with the other antibodies.

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/content/journal/jgv/10.1099/0022-1317-65-1-185
1984-01-01
2024-03-28
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References

  1. Gerhard W., Yewdell J. W., Frankel M., Lopes D., Staudt L. 1980; Monoclonal antibodies to influenza virus. In Monoclonal Antibodies p 317 Edited by Bechto K., Kennet R., McKearn T. New York: Plenum press;
    [Google Scholar]
  2. Holland J. J., Grabau E. A., Jones C. L., Semler B. L. 1979; Evolution of multiple genome mutations during long-term persistent infection by vesicular stomatitis virus. Cell 16:495–504
    [Google Scholar]
  3. Kimura Y., Ӧrvell C., Norrby E. 1979; Characterization of the polypeptides synthesized in cells infected with a temperature-sensitive mutant derived from an HVJ (Sendai virus) carrier culture. Archives of Virology 61:23–33
    [Google Scholar]
  4. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  5. Laver W. G., Gerhard W., Webster R. G., Frankel M. E., Air G. M. 1979; Antigenic drift in type A influenza virus: peptide mapping and antigenic analysis of A/PR/8/34(H0Nl) variants selected with monoclonal antibodies. Proceedings of the National Academy of Sciences, U. S. A 76:1425–1429
    [Google Scholar]
  6. Opgura H., Sato H., Hatano M. 1981; Temperature-sensitive HVJ (Sendai virus) with altered P polypeptide derived from persistently infected cell lines. Journal of General Virology 55:469–473
    [Google Scholar]
  7. Ogura H., Sato H., Hatano M. 1982; Development of protease activation mutants of HVJ (Sendai virus) in persistently infected cell cultures. Journal of General Virology 61:207–215
    [Google Scholar]
  8. Ӧrvell C., Grandien M. 1982; The effects of monoclonal antibody on biological activities of structural proteins of Sendai virus. Journal of Immunology 129:2779–2787
    [Google Scholar]
  9. Portner A. 1981; The HN glycoprotein of Sendai virus: analysis of site(s) involved in hemagglutinating and neuraminidase activities. Virology 115:375–384
    [Google Scholar]
  10. Portner A., Webster R. G., Bean W. J. 1980; Similar frequencies of antigenic variants in Sendai, vesicular stomatitis, and influenza A viruses. Virology 104:235–238
    [Google Scholar]
  11. Sato H., Ogura H., Hatano M. 1981; An intracellular interaction between a temperature-sensitive mutant and the original wild-type HVJ (Sendai virus) is responsible for the establishment and maintenance of HVJ persistent infection. Journal of General Virology 55:459–468
    [Google Scholar]
  12. Schild G. C., Oxford J. S., De Jong J. C., Webster R. G. 1983; Evidence for host-cell selection of influenza virus antigenic variants. Nature, London 303:706–709
    [Google Scholar]
  13. Ter Meulen V., Lӧffler S., Carter M. J., Stephenson J. R. 1981; Antigenic characterization of measles and SSPE virus haemagglutinin by monoclonal antibodies. Journal of General Virilogy 57:357–364
    [Google Scholar]
  14. Yewdell J. W., Gerhard W. 1982; Delineation of four antigenic sites on a paramyxovirus glycoprotein via which monoclonal antibodies mediate distinct antiviral activities. Journal oj Immunology 128:2670–2675
    [Google Scholar]
  15. Yewdell J. W., Webster R. G., Gerhard W. 1979; Antigenic variation in three distinct determinants of an influenza type A haemagglutinin molecule. Nature, London 279:246
    [Google Scholar]
  16. Yoshida T., Nagai Y., Maeno K., Iinuma M., Hamaguchi M., Matsumoto T., Nagayoshi S., Hoshino M. 1979; Studies on the role of M protein in virus assembly using a ts mutant of HVJ (Sendai virus). Virology 92:139–154
    [Google Scholar]
  17. Youngner J. S., Jones E. V., Kelly M., Frielle D. W. 1981; Generation and amplification of temperature-sensitive mutants during serial undiluted passages of vesicular stomatitis virus. Virology 108:87–97
    [Google Scholar]
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