1887

Abstract

SUMMARY

The small chain of influenza virus haemagglutinin, HA was isolated by a selective enzymic removal of HA or by preparative SDS-polyacrylamide gel electrophoresis. Anti-HA specific antisera and monoclonal antibodies were subtype-specific in immunodiffusion tests and radioimmunoassays. These antibodies did not inhibit haemagglutination or haemolysis, did not prevent virus release, did not neutralize infectivity, and HA did not induce a protective immunity. HA-specific antigenic determinants could not be demonstrated on the surface of infected cells. Lymphocytes from pre-immunized mice could not be stimulated by HA to exert a cytotoxic effect.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-1-173
1984-01-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/1/JV0650010173.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-1-173&mimeType=html&fmt=ahah

References

  1. Ada G. L., Yap K. L. 1977; Matrix protein expressed at the surface of cells infected with influenza viruses. Immunochemistry 14:643–651
    [Google Scholar]
  2. Askonas B. A., Webster R. G. 1980; Monoclonal antibodies to hemagglutinin and to H-2 inhibit the crossreactive cytotoxic T cell populations induced by influenza. European Journal of Immunology 10:151–156
    [Google Scholar]
  3. Atassi M. Z., Webster R. G. 1983; Localization, synthesis, and activity of an antigenic site on influenza virus hemagglutinin. Proceedings of the National Academy of Sciences, U. S. A 80:840–844
    [Google Scholar]
  4. Becht H., Malole B. 1975; Comparative evaluation of different fixation procedures and different coupling reagents for the demonstration of influenza virus-specific antibodies by the indirect hemagglutination test. Medical Microbiology and Immunology 162:43–53
    [Google Scholar]
  5. Bennink J. R., Yewdell J. W., Gerhard W. 1982; A viral polymerase involved in recognition of influenza virus-infected cells by a cytotoxic T-cell clone. Nature, London 296:75–76
    [Google Scholar]
  6. Biddison W. E., Doherty P. C., Webster R. G. 1977; Antibody to influenza virus matrix protein detects a common antigen on the surface of cells infected with type A influenza viruses. Journal of Experimental Medicine 146:690–696
    [Google Scholar]
  7. Bing D. H., Weyand J. G. M., Stavitsky A. B. 1967; Hemagglutination with aldehyde-fixed erythrocytes for assay of antigens and antibodies. Proceedings of the Society for Experimental Biology and Medicine 124:1166–1170
    [Google Scholar]
  8. Bosch F. X., Mayer A., Huang R. T. C. 1980; Simple and rapid separation of ortho- and paramyxovirus glycoproteins. Medical Microbiology and Immunology 168:249–259
    [Google Scholar]
  9. Braciale T. J. 1977; Immunologic recognition of influenza virus-infected cells. I. Generation of a virus-strain specific and a cross-reactive subpopulation of cytotoxic T cells in the response to type A influenza viruses of different subtypes. Cellular Immunology 33:423–436
    [Google Scholar]
  10. Brown L. E., Dopheide T. A. A., Ward C. W., White D. O., Jackson D. C. 1980; Antigenic determinants of influenza virus hemagglutinin. V. Antigenicity of HA2 chain. Journal of Immunology 125:1583–1588
    [Google Scholar]
  11. Bucher D. J., Li S. S.-L., Kehoe J. M., Kilbourne E. D. 1976; Chromatographic isolation of the hemagglutinin polypeptides from influenza virus vaccine and determination of their amino-terminal sequences. Proceedings of the National Academy of Sciences, U. S. A 73:238–242
    [Google Scholar]
  12. Effros R. B., Doherty P. C., Gerhard W., Bennink J. 1977; Generation of both cross-reactive and virus-specific T cell populations after immunization with serologically distinct influenza A viruses. Journal of Experimental Medicine 145:557–568
    [Google Scholar]
  13. Ennis A. 1982; Some newly recognized aspects of resistance against and recovery from influenza. Archives of Virology 73:207–217
    [Google Scholar]
  14. Hackett C. J., Askonas B. A., Webster R. G., Van Wyke K. 1980; Quantitation of influenza virus antigens on infected target cells and their recognition by cross-reactive cytotoxic cells. Journal of Experimental Medicine 151:1014–1025
    [Google Scholar]
  15. Huang R. T. C., Rott R., Klenk H.-D. 1981; Influenza viruses cause hemolysis and fusion of cells. Virology 110:243–247
    [Google Scholar]
  16. Jackson D. C., Brown L. E., White D. O. 1981; Antigenic determinants of influenza virus haemagglutinin. VI. Antigenic characterization of the oligosaccharide sidechains from HA1 of influenza virus haemagglutinins. Journal of General Virology 52:163–168
    [Google Scholar]
  17. Knight C. A. 1980; Immunogenic properties of PR8 influenza virus after treatment with acid protease. Intervirology 14:37–43
    [Google Scholar]
  18. Kӧhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  19. Kurrle R., Wagner H., Rӧllinghoff M., Rott R. 1979; Influenza virus-specific T cell-mediated cytotoxicity: intergration of the virus antigen into the target cell membrane is essential for target cell formation. European Journal of Immunology 9:107–111
    [Google Scholar]
  20. Laver W. G. 1971; Separation of two polypeptide chains from the hemagglutinin subunit of influenza virus. Virology 45:275–288
    [Google Scholar]
  21. Palese P., Young J. F. 1982; Variation of influenza A, B, and C viruses. Science 215:1468–1474
    [Google Scholar]
  22. Roth R. F., Arcus Y. M., Knight C. A. 1980; Proteolytic action of Aspergillus niger extract on influenza virus. Intervirology 14:167–172
    [Google Scholar]
  23. Rott R. 1977; The structural basis of the function of influenza virus glycoproteins. Medical Microbiology and Immunology 164:23–33
    [Google Scholar]
  24. Scholtissek C., Rohde W., Harms E., Rott R., Orlich M., Boschek C. B. 1978; A possible partial heterozygote of an influenza A virus. Virology 89:506–516
    [Google Scholar]
  25. Sherman L. A., Vitiello A., Klinman N. R. 1983; T-cell and B-cell responses to viral antigens at the clonal level. AnnuaI Review of Immunology 1:63–86
    [Google Scholar]
  26. Townsend A. R. M., Skehel J. J. 1982; Influenza A specific cytotoxic T-cell clones that do not recognize viral glycoproteins. Nature, London 300:655–657
    [Google Scholar]
  27. Wabuke-Bunoti M. A. N., Fan D. P. 1983; Isolation and characterization of a CNBr cleavage peptide of influenza viral hemagglutinin stimulatory for mouse cytolytic T lymphocytes. Journal of Immunology 130:2386–2391
    [Google Scholar]
  28. Ward C. W. 1981; Structure of the influenza virus hemagglutinin. Current Topics in Microbiology and Immunology 94/95:1–74
    [Google Scholar]
  29. Webster R. G., Hinshaw V. S., Bean W. J., Van Wyke K., Geraci J. R., St Aubin D. J., Petursson G. 1981; Characterization of an influenza A virus from seals. Virology 113:712–724
    [Google Scholar]
  30. Webster R. G., Laver W. G., Air G. M., Schild G. S. 1982; Molecular mechanism of variation in influenza viruses. Nature, London 296:115–121
    [Google Scholar]
  31. Wiley D. C., Wilson I. A., Skehel J. J. 1981; Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation. Nature, London 289:373–378
    [Google Scholar]
  32. Wilson I. A., Skehel J. J., Wiley D. C. 1981; Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution. Nature, London 289:366–378
    [Google Scholar]
  33. Zweerink H. J., Courtneidge S. A., Skehel J. J., Crumpton M. J., Askonas B. A. 1977; Cytotoxic T cells kill influenza virus-infected cells but do not distinguish between serologically distinct type A viruses. Nature New Biology 267:354–356
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-65-1-173
Loading
/content/journal/jgv/10.1099/0022-1317-65-1-173
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error