1887

Abstract

SUMMARY

Preparations of purified poliovirus type 1, strain Mahoney, and empty viral capsids contain a protein kinase activity. The γ-phosphoryl group of [P]ATP is transferred to all of the capsid proteins. Viral proteins phosphorylated are recognized by antiserum directed against isolated viral capsid proteins, indicating that phosphorylation does not alter the antigenic sites to such an extent that the recognition by antibodies is abolished. Viral capsid protein phosphorylation exposes new antigenic sites and leads to a destabilization of the virions. The transfer of P to viral proteins is linear for 90 min at 37 °C in the presence of 10 m-Mg at pH 8.1. Reducing agents or virus-stabilizing agents (such as arildone) reduce the kinase activity and result in a different pattern of capsid protein phosphorylation.

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1984-01-01
2022-08-17
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References

  1. Akusjärvi G., Philipson L., Petterson U. 1978; A protein kinase associated with adenovirus type 2. Virology 87:276–286
    [Google Scholar]
  2. Blue W. T., Stubbs D. G. 1981; Isolation of a protein kinase induced by herpes simplex virus type 1. Journal of Virology 38:383–388
    [Google Scholar]
  3. Breindl M. 1971; The structure of heated poliovirus particles. Journal of General Virology 11:147–156
    [Google Scholar]
  4. Caliguiri L. A., Mcsharry J. I., Lawrence G. W. 1980; Effect of arildone on modifications of poliovirus in vitro. Virology 105:86–93
    [Google Scholar]
  5. De Haro C., Manne V., De Herreros A. G., Ochoa S. 1982; Heat-stable inhibitor of translation in reticulocyte lysates. Proceedings of the National Academy of Sciences, U. S. A 79:3134–3137
    [Google Scholar]
  6. Downer D. N., Rogers H. W., Randall C. C. 1973; Endogenous protein kinase and phosphate acceptor proteins in vaccinia virus. Virology 52:13–21
    [Google Scholar]
  7. Drzeniek R., Bilello P. 1972; Reconstruction of poliovirus. Biochemical and Biophysical Research Communications 46:719–724
    [Google Scholar]
  8. Fenwick M. L., Wall M. J. 1973; Factors determining the site of synthesis of poliovirus proteins: the early attachment of virus particles to endoplasmic membranes. Journal of Cell Science 13:403–413
    [Google Scholar]
  9. Grubman M. J., Baxt B., La Torre J. L., Bachrach H. L. 1981; Identification of a protein kinase activity in purified foot-and-mouth disease virus. Journal of Virology 39:455–462
    [Google Scholar]
  10. Habermehl K. O., Diefenthal W., Buchholz M. 1974; Distribution of parental viral constituents in the course of polio infection. In Advances in the Biosciences vol 11: pp 41–63 Edited by Raspé G. Vieweg & Oxford: Pergamon Press;
    [Google Scholar]
  11. James L. A., Tershak D. R. 1981; Protein phosphorylation in poliovirus-infected cells. Canadian Journal of Microbiology 27:28–35
    [Google Scholar]
  12. Koch F., Koch G., Kruppa J. 1982; Virus-induced shut-off of host specific protein synthesis. In Protein Biosynthesis in Eukaryotes pp 339–361 Edited by Perez-Bercoff R. New York: Plenum Press;
    [Google Scholar]
  13. Koch G., Quintrell N., Bishop J. M. 1966; An agar-cell suspension plaque assay for isolated viral RNA. Biochemical and Biophysical Research Communications 24:304–309
    [Google Scholar]
  14. Krebs E. G., Beavo J. A. 1979; Phosphorylation-dephosphorylation of enzymes. Annual Review of Biochemistry 48:923–959
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  16. La Torre J. L., Grubman M. J., Baxt B., Bachrach H. L. 1980; The structural polypeptides of aphthovirus are phosphoproteins. Proceedings of the National Academy of Sciences, U. S. A 77:7444–7447
    [Google Scholar]
  17. Lemaster S., Roizman B. 1980; Herpes simplex virus phosphoproteins. II. Characterization of the virion protein kinase and of the polypeptides phosphorylated in the virus. Journal of Virology 35:798–811
    [Google Scholar]
  18. Lonberg-Holm K., Philipson L. 1974; Early interaction between animal viruses and cells. In Monographs in Virology vol 9: pp 1–148 Edited by Melnick J. L. Basel: S. Karger;
    [Google Scholar]
  19. Lonberg-Holm K., Whitely N. M. 1976; Physical and metabolic requirements for early interaction of poliovirus and human rhinovirus with HeLa cells. Journal of Virology 19:857–870
    [Google Scholar]
  20. Meloen R. H., Rowlands D. J., Brown F. 1979; Comparison of the antibodies elicited by the individual structural polypeptides of foot-and-mouth disease and polio viruses. Journal of General Virology 45:761–763
    [Google Scholar]
  21. Racevskis J., Koch G. 1978; Synthesis and processing of viral proteins in Friend erythroleukemia cell lines. Virology 87:345–365
    [Google Scholar]
  22. Racevskis J., Kerwar S. S., Koch G. 1976; Inhibition of protein synthesis in reticulocyte lysates by poliovirus. Journal of General Virology 31:135–138
    [Google Scholar]
  23. Ranu R. S., London I. M., Das A., Dasgupta A., Majumpar A., Ralston R., Roy R., Gupta N. K. 1978; Regulation of protein synthesis in rabbit reticulocytes by the heme-regulated protein kinase: inhibition of met-tRNAfmet binding factor with another initiation factor in formation of met-tRNAfmat 40S ribosomal subunit complexes. Proceedings of the National Academy of Sciences, U. S. A 75:745–749
    [Google Scholar]
  24. Rosen O. M. Krebs E. G. 1981; Protein synthesis. In Protein Phosphorylation Cold Spring Harbor Conferences on Cell Proliferation vol 8: pp 931–1013 Edited by Rosen O. M., Krebs E. G. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Rueckert R. R. 1976; On the structure and morphogenesis of picornaviruses. In Comprehensive Virology vol 6: pp 131–213 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  26. Schärli C. E., Koch G. 1981; Modification of poliovirus capsid proteins. Zentralblatt fur Bakteriologie und Hygiene, Abteilung 1 Originale A251:414
    [Google Scholar]
  27. Silberstein H., August J. T. 1973; Phosphorylation of animal virus proteins by a virion protein kinase. Journal of Virology 12:511–522
    [Google Scholar]
  28. Sonnenberg N., Rupprecht K. M., Hecht S. M., Shatkin A. J. 1979; Eukaryotic mRNA cap binding protein: purification by affinity chromatography on Sepharose-coupled m7GDP. Proceedings of the National Academy of Sciences, U. S. A 76:4345–4349
    [Google Scholar]
  29. Strand M., August J. T. 1971; Protein kinase and phosphate acceptor proteins in Rauscher murine leukaemia virus. Nature New Biology 233:137–140
    [Google Scholar]
  30. Tan K. B., Sokol F. 1974; Bound protein kinase in Semliki Forest and Sindbis viruses. Journal of Virology 13:1245–1253
    [Google Scholar]
  31. Trachsel H., Gordon J., Rose J. K., Leong K., Baltimore D., Sonnenberg N., Shatkin A. J. 1979; Cap binding protein restores translation of capped mRNAs in extracts of poliovirus-infected cells. Proceedings of the Xlth International Congress of Biochemistry134
    [Google Scholar]
  32. Van Elsen A., Boeye A. 1966; Disruption of type 1 poliovirus under alkaline conditions: role of pH, temperature and sodium dodecyl sulfate (SDS). Virology 28:481–483
    [Google Scholar]
  33. Witt D. J., Summers D. F. 1980; Relationship between virion-associated kinase-effected phosphorylation and transcription activity of vesicular stomatitis virus. Virology 107:34–49
    [Google Scholar]
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