1887

Abstract

SUMMARY

RNAs of soil-borne wheat mosaic virus (SBWMV) from virions 281 nm, 138 nm and 92 nm long (designated here by relative lengths as 1.0L, 0.5L and 0.35L, respectively), were isolated and purified by three cycles of sucrose density gradient centrifugation. Infectivity assays with these RNAs proved the bipartite nature of SBWMV, the combination of 1.0L and either 0.5L or 0.35L RNAs being required for infection and for multiplication of progeny viruses. The 0.5L RNA underwent deletion mutation, producing smaller variants with various sizes, of which 0.4L and 0.35L RNAs were confirmed to be functional in combination with 1.0L RNA. The coat proteins of all isolates had mol. wt. of 19700. The mol. wt. of 1.0L, 0.5L, 0.4L and 0.35L RNAs, determined under denaturing conditions, were 2.28 × 10 (6500 bases), 1.23 × 10 (3500 bases), 0.97 × 10 (2800 bases) and 0.86 × 10 (2450 bases), respectively. A new virus group, furovirus (fungus-borne rod-shaped virus), is proposed for SBWMV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-1-119
1984-01-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/1/JV0650010119.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-1-119&mimeType=html&fmt=ahah

References

  1. Adam G., Sander E., Shepherd R. J. 1979; Structural differences between pea enation mosaic virus strains affecting transmissibility by Acrythosiphon pisum (Harris). Virology 92:1–14
    [Google Scholar]
  2. Boedtker H. 1971; Conformation-independent molecular weight determinations of RNA by gel electrophoresis. Biochimica et biophysica acta 240:448–453
    [Google Scholar]
  3. Brakke M. K. 1971; Soil-borne wheat mosaic virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses no. 77
    [Google Scholar]
  4. Brakke M. K. 1977; Sedimentation coefficients of the virions of soil-borne wheat mosaic virus. Phytopathology 67:1433–1438
    [Google Scholar]
  5. Brakke M. K., Van Pelt N. 1970; Properties of infectious ribonucleic acid from wheat streak mosaic virus. Virology 42:699–706
    [Google Scholar]
  6. Brakke M. K., Estes A. P., Schuster M. L. 1965; Transmission of soil-borne wheat mosaic virus. Phytopathology 55:79–86
    [Google Scholar]
  7. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences, U. S. A 75:4801–1805
    [Google Scholar]
  8. Brosius J., Dull T. J., Noller H. F. 1980; Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences, U. S. A 77:201–204
    [Google Scholar]
  9. Brunt A. A., Stace-Smith R. 1978; Some hosts, properties and possible affinities of a labile virus from Hypochoeris radicata (Compositae). Annals of Applied Biology 90:205–214
    [Google Scholar]
  10. Campbell L. G., Heyne E. G., Gronau D. M., Niblett C. 1975; Effect of soil-borne wheat mosaic virus on wheat yield. Plant Disease Reporter 59:472–476
    [Google Scholar]
  11. Casper D. L. D. 1963; Assembly and stability of the tobacco mosaic virus particle. Advances in Protein Chemistry 18:37–121
    [Google Scholar]
  12. Dahlberg A. E., Dingman C. W., Peacock A. C. 1969; Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels. Journal of Molecular Biology 41:139–147
    [Google Scholar]
  13. Estes A. P., Brakke M. K. 1966; Correlation of Polymyxa graminis with transmission of soil-borne wheat mosaic virus. Virology 28:772–774
    [Google Scholar]
  14. Gibbs A. J. 1977; Tobamovirus group. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses no. 184
    [Google Scholar]
  15. Goelet P., Lomonossoff G. P., Butler P. I. G., Akam M. E., Gait M. J., Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, U. S. A 79:5818–5822
    [Google Scholar]
  16. Greber R. S., Finlay I. R. 1981; Hypochoeris mosaic virus in Australia. Australian Plant Pathology 10:30–31
    [Google Scholar]
  17. Gumpf D. J. 1971; Purification and properties of soil-borne wheat mosaic virus. Virology 43:588–596
    [Google Scholar]
  18. Harrison B. D. 1974; Potato mop-top virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses no. 138
    [Google Scholar]
  19. Hibino H., Tsuchizaki T., Saito Y. 1981; Influence of host species and infection stages on the components of soil-borne wheat mosaic virus. Annals of the Phytopathological Society of Japan 47:566–570
    [Google Scholar]
  20. Howarth A. J., Gardner R. C., Messing J., Shepherd R. J. 1981; Nucleotide sequence of naturally occurring deletion mutants of cauliflower mosaic virus. Virology 112:678–685
    [Google Scholar]
  21. Hsu Y. H., Brakke M. K. 1983; Translation of soil-borne wheat mosaic virus RNAs in rabbit reticulocyte lysate (abstract). Phytopathology 73:790
    [Google Scholar]
  22. Hull R., Howell S. H. 1978; Structure of the cauliflower mosaic virus genome. II. Variation in DNA structure and sequence between isolates. Virology 86:482–493
    [Google Scholar]
  23. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  24. Lane L. C. 1978; A simple method for stabilizing protein-sulfhydryl groups during SDS-gel electrophoresis. Analytical Biochemistry 86:655–664
    [Google Scholar]
  25. Lehrach H., Diamond D., Wozney J. M., Boedtker H. 1977; RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743–4751
    [Google Scholar]
  26. Mcmaster G. K., Carmichael G. C. 1977; Analysis of single- and double-stranded nucleic acids in polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U. S. A 74:4835–4838
    [Google Scholar]
  27. Palmer L. T., Brakke M. K. 1975; Yield reduction in winter wheat infected with soil-borne wheat mosaic virus. Plant Disease Reporter 59:469–471
    [Google Scholar]
  28. Peacock A. C., Dingman C. W. 1968; Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7:668–674
    [Google Scholar]
  29. Powell C. A. 1976; The relationship between soil-borne wheat mosaic virus and tobacco mosaic virus. Virology 71:453–162
    [Google Scholar]
  30. Putz C. 1977; Composition and structure of beet necrotic yellow vein virus. Journal of General Virology 35:397–401
    [Google Scholar]
  31. Randles J. W., Harrison B. D., Roberts I. M. 1976; Nicotiana velutina mosaic virus: purification, properties and affinities with other rod-shaped viruses. Annals of Applied Biology 84:193–204
    [Google Scholar]
  32. Rao A. S., Brakke M. K. 1969; Relation of soil-borne wheat mosaic virus and its fungal vector, Polymyxa graminis. Phytopathology 59:581–587
    [Google Scholar]
  33. Rao A. S., Brakke M. K. 1970; Dark treatment of wheat inoculated with soil-borne wheat mosaic and barley stripe mosaic viruses. Phytopathology 60:714–716
    [Google Scholar]
  34. Reddy D. V. R., Black L. M. 1974; Deletion mutations of the genome segments of wound tumor virus. Virology 61:458–473
    [Google Scholar]
  35. Roberts I. M., Harrison B. D. 1979; Detection of potato leafroll and potato mop-top viruses by immunosorbent electron microscopy. Annals of Applied Biology 93:289–297
    [Google Scholar]
  36. Tamada T. 1975; Beet necrotic yellow vein virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses no. 144
    [Google Scholar]
  37. Thouvenel J. C., Dollet M., Fauquet C. 1976; Some properties of peanut clump, a newly discovered virus. Annals of Applied Biology 84:311–320
    [Google Scholar]
  38. Tsuchizaki T., Hibino H., Saito Y. 1973; Comparisons of soil-borne wheat mosaic virus isolates from Japan and the United States. Phytopathology 63:634–639
    [Google Scholar]
  39. Tsuchizaki T., Hibino H., Saito Y. 1975; The biological functions of short and long particles of soil-borne wheat mosaic virus. Phytopathology 65:523–532
    [Google Scholar]
  40. Zaitlin M., Isarel H. W. 1975; Tobacco mosaic virus (type strain). Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses no. 151
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-1-119
Loading
/content/journal/jgv/10.1099/0022-1317-65-1-119
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error