1887

Abstract

SUMMARY

Two RNA species, and one DNA species, were isolated from subterranean clover red leaf virus (SCRLV) prepared by a modification of previously described methods. H-labelled cDNA transcribed from high molecular weight RNA of purified virus was specific for the detection of SCRLV, in that it showed no hybridization with nucleic acids from either healthy plants, or plants infected with the serologically related potato leaf roll virus, but hybridized with homologous RNA and nucleic acids from SCRLV-infected plants of two species. The cDNA detected SCRLV in individuals and groups of the aphid vector, , and the average virus content was greater than 170 pg per aphid. The non-vector, , contained only trace amounts of SCRLV, a result confirmed by enzyme-linked immunosorbent assay.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-1-109
1984-01-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/1/JV0650010109.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-1-109&mimeType=html&fmt=ahah

References

  1. Aapola A. E. I., Rochow W. F. 1968; Immunodiffusion tests with three isolates of barley yellow dwarf virus. Phytopathology 58:398
    [Google Scholar]
  2. Abu-Samah N., Randles J. W. 1981; A comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other polyviruses. Virology 110:436–444
    [Google Scholar]
  3. Abu-Samah N., Randles J. W. 1983; A comparison of Australian bean yellow mosaic virus isolates using molecular hybridisation analysis. Annals of Applied Biology 103:97–107
    [Google Scholar]
  4. Ashby J. W., Kyriakou A. 1982; Purification and properties of subterranean clover red leaf virus. New Zealand Journal of Agricultural Research 25:607–612
    [Google Scholar]
  5. Ashby J. W., Teh P. B., Close R. C. 1979; Symptomatology of subterranean clover red leaf virus and its incidence in some legume crops, weed hosts and certain alate aphids in Canterbury, New Zealand. New Zealand Journal of Agricultural Research 22:361–365
    [Google Scholar]
  6. Boccardo G., Beaver R. G., Randles J. W., Imperial J. S. 1981; Tinangaja and bristle top, coconut diseases of uncertain etiology in Guam, and their relationship to cadang-cadang disease of coconut in the Philippines. Phytopathology 71:1104–1107
    [Google Scholar]
  7. Falk B. W., Duffus J. E., Morris T. J. 1977; Two RNA species isolated from purified beet western yellows virus. Proceedings of the American Phytopathological Society 4:160
    [Google Scholar]
  8. Gonda T. J., Symons R. H. 1978; The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: its application to several strains of cucumoviruses. Virology 88:361–370
    [Google Scholar]
  9. Gould A. R., Francki R. I. B. 1981; Immunochemical detection of ds-RNA in healthy and virus-infected plants and specific detection of viral ds-RNA by hybridization to labelled complementary DNA. Journal of Virological Methods 2:277–286
    [Google Scholar]
  10. Gould A. R., Symons R. H. 1977; Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA. Nucleic Acids Research 4:3787–3802
    [Google Scholar]
  11. Jayasena K. W., Randles J. W. 1983; Patterns of spread of the non-persistently transmitted bean yellow mosaic virus and the persistently transmitted subterranean clover red leaf virus in Vicia faba. Annals of Applied Biology (in press)
    [Google Scholar]
  12. Jayasena K. W., Hatta T., Francki R. I. B., Randles J. W. 1981; Luteovirus-like particles associated with subterranean clover red leaf virus infection. Journal of General Virology 57:205–209
    [Google Scholar]
  13. Johnstone G. R. 1978; Diseases of broad bean (Vicia faba L. major) and green pea (Pisum sativum L.) in Tasmania caused by subterranean clover red leaf virus. Australian Journal of Agricultural Research 29:1003–1010
    [Google Scholar]
  14. Johnstone G. R., Duffus J. E., Munro D., Ashby J. W. 1982; Purification of a Tasmanian isolate of subterranean clover red leaf virus, and its serological interactions with a New Zealand isolate and other luteoviruses. Australian Journal of Agricultural Research 33:697–703
    [Google Scholar]
  15. Kellock A. W. 1971; Red-leaf virus-a newly recognized virus disease of subterranean clover (Trifolium subterraneum L.). Australian Journal of Agricultural Research 22:615–624
    [Google Scholar]
  16. McLaughlin M. R., Barnett O. W., Burrows P. M., Baum R. H. 1981; Improved ELISA conditions for detection of plant viruses. Journal of Virological Methods 3:13–25
    [Google Scholar]
  17. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  18. Owens R. A., Cress D. E. 1980; Molecular cloning and characterization of potato spindle tuber viroid cDNA sequences. Proceedings of the National Academy of Sciences, U. S. A 77:5302–5306
    [Google Scholar]
  19. Palukaitis P., Symons R. H. 1980; Nucleotide sequence homology of thirteen tobamovirus RNAs as determined by hybridization analysis with complementary DNA. Virology 107:354–361
    [Google Scholar]
  20. Palukaitis P., Rakowski A. G., Alexander D. M., Symons R. H. 1981; Rapid indexing of the sunblotch disease of avocados using a complementary DNA probe to avocado sunblotch viroid. Annals of Applied Biology 98:439–449
    [Google Scholar]
  21. Peacock A. C., Dingman C. W. 1968; Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7:668–674
    [Google Scholar]
  22. Ralph R., Bellamy A. R. 1964; Isolation and purification of undegraded ribonucleic acids. Biochimica et biophysica acta 119:29–36
    [Google Scholar]
  23. Randles J. W. 1975; Association of two ribonucleic acid species with cadang-cadang disease of coconut palm. Phytopathology 65:163–167
    [Google Scholar]
  24. Randles J. W., Coleman D. F. 1970; Loss of ribosomes in Nicotiana glutinosa L. infected with lettuce necrotic yellows virus. Virology 41:459–464
    [Google Scholar]
  25. Randles J. W., Davies C., Hatta T., Gould A. R., Francki R. I. B. 1981; Studies on encapsidated viroid-like RNA. I. Characterization of velvet tobacco mottle virus. Virology 108:111–122
    [Google Scholar]
  26. Reddick B. B., Barnett O. W. 1983; A comparison of three potyviruses using direct hybridization analysis. Phytopathology (in press)
    [Google Scholar]
  27. Roberts I. M., Tamada T., Harrison B. D. 1980; Relationship of potato leafroll virus to luteoviruses: evidence from electron microscope serological tests. Journal of General Virology 47:209–213
    [Google Scholar]
  28. Rowhani A., Stace-Smith R. 1979; Purification and characterization of potato leafroll virus. Virology 98:45–54
    [Google Scholar]
  29. Sarkar S. 1976; Potato leaf roll virus contains a double-stranded DNA. Virology 70:265–273
    [Google Scholar]
  30. Tamada T., Harrison B. D. 1980; Application of enzyme-linked immunosorbent assay to the detection of potato leafroll virus in potato tubers. Annals of Applied Biology 96:67–78
    [Google Scholar]
  31. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:324–330
    [Google Scholar]
  32. Van Der Walle M. J., Siegel A. 1982; Relationships between strains of tobacco mosaic virus and other selected plant viruses. Phytopathology 72:390–395
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-1-109
Loading
/content/journal/jgv/10.1099/0022-1317-65-1-109
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error