RT Journal Article SR Electronic(1) A1 Suh, Martha A1 Chauvin, Christiane A1 Filion, Mario A1 Shore, Gordon C. A1 Frost, EricYR 1983 T1 Localization of the Coding Region for a 35000 Dalton Polypeptide on the Genome of Herpes Simplex Virus Type 2 JF Journal of General Virology, VO 64 IS 9 SP 2079 OP 2085 DO https://doi.org/10.1099/0022-1317-64-9-2079 PB Microbiology Society, SN 1465-2099, AB SUMMARY The cloned BglII N fragment of herpes simplex virus type 2 (HSV-2) DNA has been shown to code for a 35K polypeptide. Subfragments made by cleavage with XhoI, BamHI, SstII and XorII were then cloned and used with RNA extracted from HSV-2-infected cells for mRNA selection and in vitro protein synthesis. We found that the major translation product of such hybrid-selected mRNA has a molecular weight of 35000. By further mapping, DNA coding sequences for this mRNA were located within the region of BglII N, at approximately 0.585 to 0.596 genome map units. DNA sequences complementary to mRNA encoding a 56K polypeptide were located between 0.607 and 0.612 map units., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-9-2079