N-Acetyl-tyrosine at the 3′ End of Brome Mosaic Virus RNA has Little Effect on Infectivity

Digestion of the N-acetylated-tyrosylated form of brome mosaic virus (BMV) RNA with proteinase K removed the 3′-blocking N-acetyl-tyrosine residue. Removal of the blocking group affected neither infectivity nor mRNA activity. The previously reported effect of acetylation of tyrosylated BMV RNA on infectivity appears to result from non-specific acetylation of the RNA.