1887

Abstract

SUMMARY

Effects of carrageenan and γ-irradiation on virus titre in the liver were observed after intravenous inoculation of 8 × 10 p.f.u. of ectromelia virus which was not lethal for untreated mice. Trapping of virus by the liver within 30 min and an initial transient reduction in titre by day 1 were not affected by γ-irradiation but were inhibited by pretreatment with carrageenan. An increase from day 1 to day 3 was not affected by γ-irradiation but was augmented by pretreatment with carrageenan. Therefore, protection within 3 days may depend principally upon carrageenan-sensitive and irradiation-resistant cells, namely, fixed macrophages. Elimination of virus from day 4 to day 7 depended upon cell-mediated immunity. When carrageenan was given 3 days after virus inoculation, the titre of virus increased progressively from day 4 ultimately to kill the hosts. The cytotoxic activity of spleen cells against infected target cells was raised in carrageenan-treated mice as well as in untreated mice. Immune elimination of virus may be mediated by a mechanism requiring the cooperation of sensitized T lymphocytes and blood monocytes.

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1983-09-01
2024-04-18
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References

  1. Benacerraf B., Kivy-Rosenberg E., Sebestyen M. M., Zweifach B. W. 1959; The effect of high doses of X-irradiation on the phagocytic, proliferative and metabolic properties of the reticuloendothelial system. Journal of Experimental Medicine 110:49–63
    [Google Scholar]
  2. Berlin B. S. 1964; Sparing effect of X-rays for mice inoculated intranasally with egg-adapted influenza virus, CAM strain. Proceedings of the Society for Experimental Biology and Medicine 177:864–869
    [Google Scholar]
  3. Blanden R. V., Gardner I. D. 1976; The cell-mediated immune response to ectromelia virus infection. I.Kinetics and characteristics of the primary effector T cell response in vivo. Cellular Immunology 22:271–282
    [Google Scholar]
  4. Catanzaro P. J., Schwartz H. J., Graham R. C. 1971; Spectrum and possible mechanism of carrageenan cytotoxicity. American Journal of Pathology 64:387–404
    [Google Scholar]
  5. Cole G. A., Nathanson N. 1968; Potentiation of experimental arbovirus encephalitis by immunosuppressive doses of cyclophosphamide. Nature, London 220:399–401
    [Google Scholar]
  6. Degara P. F., Furth J. 1945; The relative susceptibility of normal and X-rayed mice of different stocks to pneumotropic viruses. Journal of Immunology 50:255–264
    [Google Scholar]
  7. Gardner L., Bowern N. A., Blanden R. V. 1974; Cell-mediated cytotoxicity against ectromelia virus-infected target cells. I. Specificity and kinetics. European Journal of Immunology 4:63–67
    [Google Scholar]
  8. Gillette R. W., Lance E. M. 1973; Kinetic studies of macrophages. IV. Effect of irradiation. Journal of the Reticuloendothelial Society 14:18–25
    [Google Scholar]
  9. Heremans H., Billiau A., De Somer P. 1980; Interferon in experimental viral infections in mice: tissue interferon levels resulting from the virus infection and from exogenous interferon therapy. Infection and Immunity 30:513–522
    [Google Scholar]
  10. Hurd J., Heath R. B. 1975; Effect of cyclophosphamide on infections in mice caused by virulent and avirulent strains of influenza virus. Infection and Immunity 11:886–889
    [Google Scholar]
  11. Kornfeld L., Greenman V. 1966; Effect of total body X-irradiation on peritoneal cells of mice. Radiation Research 29:433–444
    [Google Scholar]
  12. Nelson D. S. 1969 Macrophage and Immunity Amsterdam: North-Holland;
    [Google Scholar]
  13. Robinson T. W. E., Cureton R. J. R., Heath R. B. 1969; The effect of cyclophosphamide on Sendai virus infection of mice. Journal of Medical Microbiology 2:137–145
    [Google Scholar]
  14. Singer S. H., Noguchi P., Kirschstein R. L. 1972; Respiratory disease in cyclophosphamide-treated mice. II.Decreased virulence of PR 8 influenza virus. Infection and Immunity 5:957–960
    [Google Scholar]
  15. Stuart A. E., Habeshaw J. A., Davidson A. E. 1973; Phagocytes in vitro. In Handbook of Experimental Immunology 2nd edn pp 3125–3128 Edited by M D. Weir, Oxford: Blackwell;
    [Google Scholar]
  16. Tatsukawa K., Mitsuyama M., Takeya K., Nomoto K. 1979; Differing contribution of polymorphonuclear cells and macrophages to protection of mice against Listeria monocytogenes and Pseudomonas aeruginosa. Journal of General Microbiology 115:161–166
    [Google Scholar]
  17. Thind I. S., Price W. H. 1968; Cross protection with group B arboviruses in mice treated with cyclophosphamide: role of serum antibody, viremia, and virus multiplication in the brain. American Journal of Epidemiology 89:89–97
    [Google Scholar]
  18. Thind I. S., Price W. H. 1969; Recovery from primary infection with E5 virus in normal and cyclophosphamide-treated mice: relative roles of virus multiplication, interferon, antibody and serum protective factor. American Journal of Epidemiology 89:593–605
    [Google Scholar]
  19. Volkman A., Collins F. M. 1971; The restorative effect of peritoneal macrophages on delayed hypersensitivity following ionizing radiation. Cellular Immunology 2:552–566
    [Google Scholar]
  20. Werner G. T., Jentzsch U., Metzger E., Simon J. 1980; Studies on poxvirus infections in irradiated animals. Archives of Virology 64:247–256
    [Google Scholar]
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