Skip to content
1887

Journal of General Virology header logo Journal of General Virology

Volume 64, Issue 9

Genetic Determinants Distributed in Two Genomic RNAs of Sweet Clover Necrotic Mosaic, Red Clover Necrotic Mosaic and Clover Primary Leaf Necrosis Viruses No Access

Abstract

SUMMARY

Three serologically distinct polyhedral viruses, sweet clover necrotic mosaic virus (SCNMV), red clover necrotic mosaic virus (RCNMV) and clover primary leaf necrosis virus (CPLNV), contain bipartite RNAs with approximate mol. wt. of 1.35 × 10 to 1.55 × 10 (RNA 1) and 0.5 × 10 to 0.6 × 10 (RNA 2). The homologous and heterologous combinations of RNA 1 and RNA 2 of the three viruses were highly infectious, while individual RNA species were not. Electrophoretic mobility of genomic RNA from the pseudorecombinants showed that the progeny viruses maintained the heterologous RNA combinations identically with those in the original inocula. Serological specificity of the progeny viruses was determined by RNA 1. A mixture of isolated coat protein and RNA 1 was not infectious. RNA 1 of SCNMV was essential for systemic infection of sweet clover at 26 °C, while RNA 2 of SCNMV complemented RNA 1 of RCNMV in causing local infection in sweet clover at 26 °C. The heterologous combinations of CPLNV RNA 1 and RNA 2 of SCNMV or of RCNMV acquired the ability to infect white clover at 26 °C and also caused symptoms characteristically different from those induced by their parental viruses on L. cv. Red Kidney.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): coat protein , dianthoviruses , genome , legume viruses and pseudorecombinants
© Journal of General Virology 1983
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-9-1907
1983-09-01
2025-01-17
Loading full text...

Full text loading...

References

  1. Bruening G. 1977; Plant covirus system: two-component systems. In Comprehensive Virology vol 11: pp 55–61 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
     [Google Scholar]
  2. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
     [Google Scholar]
  3. Dodds J. A., Tremaine J. H., Ronald W. P. 1977; Some properties of carnation ringspot virus single- and double-stranded ribonucleic acid. Virology 83:322–328
     [Google Scholar]
  4. Gould A. R., Francki R. I. B., Hatta T., Hollings M. 1981; The bipartite genome of red clover necrotic mosaic virus. Virology 108:499–506
     [Google Scholar]
  5. Hiruki C., Okuno T., Rao D. V., Chen M. H. 1981; A new bipartite genome virus, sweet clover necrotic mosaic virus occurring in Alberta. A workshop paper on novel plant viruses. Abstracts, Fifth International Congress of Virology p 235 (Strasbourg, France)
    [Google Scholar]
  6. Hollings M., Stone O. M. 1979; Viruses of forage crops. Annual Report, The Glasshouse Crops Research Institute pp 160–161 (Littlehampton, U.K.)
    [Google Scholar]
  7. Hull R., Lane L. C. 1973; The unusual nature of the components of a strain of pea enation mosaic virus. Virology 55:1–13
     [Google Scholar]
  8. Matkin O. A., Chandler P. A. 1957; The U.C.-type soil mixtures. In The U.C. System for Producing Healthy Container-Grown Plants pp 68–85 Edited by Baker K. F. California Agricultural Experiment Station Service Manua, No. 23;
     [Google Scholar]
  9. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
     [Google Scholar]
  10. Okuno T., Furusawa I. 1979; RNA polymerase activity and protein synthesis in brome mosaic virus-infected protoplasts. Virology 99:218–225
     [Google Scholar]
  11. Ragetli H. W. J., Elder M. 1977; Characteristics of clover primary leaf necrosis virus, a new spherical isolate from Trifolium pratense. Canadian Journal of Botany 55:2122–2136
     [Google Scholar]
  12. Shepherd R. J., Wakeman R. J., Ghabriel S. A. 1968; Preparation and properties of the protein and nucleic acid components of pea enation mosaic virus. Virology 35:255–267
     [Google Scholar]
  13. Van Vloten-Doting L., Jaspers E. J. 1977; Plant covirus system: three-component systems. In Comprehensive Virology vol 11 pp 1–53 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-64-9-1907
Loading
Genetic Determinants Distributed in Two Genomic RNAs of Sweet Clover Necrotic Mosaic, Red Clover Necrotic Mosaic and Clover Primary Leaf Necrosis Viruses
J. Gen. Virol. 64, 1907 (1983); https://doi.org/10.1099/0022-1317-64-9-1907
/content/journal/jgv/10.1099/0022-1317-64-9-1907
/content/journal/jgv/10.1099/0022-1317-64-9-1907
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error