1887

Journal of General Virology header logo

Volume 64, Issue 9

Antibodies to Respiratory Syncytial Virus Polypeptides and their Significance in Human Infection Free

Abstract

SUMMARY

The human antibody response to respiratory syncytial (RS) virus infection was investigated using radioimmunoprecipitation analysis (RIPA). A total of nine RS virus-specific proteins, VP200, VGP95, VP68, VGP48, VPN41, VP35, VP27, VP23 and VGP20 were identified by comparing S- or H-labelled extracts of infected and uninfected HEp-2 cells, and by radioimmunoprecipitation using a hyperimmune human serum. Three glycopeptides, VGP95, VGP48 and VGP20, were identified by incorporation of [H]glucosamine, and two of these (VGP48 and VGP20) were assumed to be part of a single disulphide-bonded polypeptide since they were precipitated by a monoclonal antibody raised against a surface protein. Human serum antibodies to three major RS virus proteins, VGP95, VGP48/VGP20 and VPN41 were measured by RIPA using radioiodinated RS virus antigens. Sera from a group of mothers whose babies escaped RS virus infection during a local epidemic showed increased antibody levels to VPN41 when compared to sera from mothers whose babies had become infected with RS virus within the first 6 months of life. In infants who remained uninfected with RS virus during the first 12 months of life the maternal gift of antibody decayed to about 50% at 3 months with traces of antibodies detected in a few sera at 12 months. The antibody levels detected in the sera of infants less than 3 months old convalescent from primary RS virus infection did not exceed the mean levels present in the serum of uninfected babies. Infants between the ages of 6 and 12 months were able to mount an IgG response to VPN41 and VGP48 but, unlike adults and older children, a particularly striking finding was their failure to produce antibodies to VGP95.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Ab response , infection , polypeptides and RS virus
© Journal of General Virology 1983
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-9-1867
1983-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/9/JV0640091867.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-9-1867&mimeType=html&fmt=ahah

References

  1. Belshe R. B., Van Voris L. P., Mufson M. A. 1982; Parenteral administration of live respiratory syncytial virus vaccine: results of field trial. Journal of Infectious Diseases 145:311–319
     [Google Scholar]
  2. Bernstein J. M., Hruska I. F. 1981; Respiratory syncytial virus proteins: identification by immunoprecipitation. Journal of Virology 38:278–285
     [Google Scholar]
  3. Chanock R. M., Kapikian A. Z., Mills J., Kim H. W., Parrott R. H. 1970; Influence of immunological factors in respiratory syncytial virus disease of the lower respiratory tract. Archives of Environmental Health 21:347–355
     [Google Scholar]
  4. Dubovi E. J. 1982; Analysis of proteins synthesized in respiratory syncytial virus-infected cells. Journal of Virology 42:372–378
     [Google Scholar]
  5. Fairbanks G., Steck T. L., Wallach D. F. H. 1971; Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617
     [Google Scholar]
  6. Fazekas De ST. Groth S., Scheidegger D. 1980; Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 35:1–21
     [Google Scholar]
  7. Fernie B. F., Gerin J. L. 1982; Immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion. Infection and Immunity 37:243–249
     [Google Scholar]
  8. Gardner P. S. 1973; Respiratory syncytial virus infections. Post-Graduate Medical Journal 49:788–791
     [Google Scholar]
  9. Glezen W. P., Paredes A., Allison J. E., Taber L. H., Frank A. Z. 1981; Risk of respiratory syncytial virus infection for infants from low-income families in relationship to age, sex, ethnic group and maternal antibody level. Journal of Pediatrics 98:708–715
     [Google Scholar]
  10. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of 131I-labelled human growth hormone of high specific radioactivity. Biochemical Journal 89:114–123
     [Google Scholar]
  11. Gruber C., Levine S. 1983; Respiratory syncytial virus polypeptides. III. The envelope-associated proteins. Journal of General Virology 64:825–832
     [Google Scholar]
  12. Heckels J. E. 1981; Structural comparison of Neisseria gonorrhoeae outer membrane proteins. Journal of Bacteriology 145:736–742
     [Google Scholar]
  13. Huang Y. T., Wertz G. W. 1982; The genome of respiratory syncytial virus is a negative-stranded RNA that codes for at least seven mRNA species. Journal of Virology 43:150–157
     [Google Scholar]
  14. Jen G., Thach R. E. 1982; Inhibition of host translation in encephalomyocarditis virus-infected L cells: a novel mechanism. Journal of Virology 43:250–261
     [Google Scholar]
  15. Kim H. W., Canchola J. G., Brandt C. D., Pyles G., Chanock R. M., Jensen K., Parrott R. H. 1969; Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine. American Journal of Epidemiology 89:422–434
     [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  17. Marchalonis J. J., Cone R. E., Santer V. 1971; Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes. Biochemical Journal 124:921–927
     [Google Scholar]
  18. Neligan G. A., Steiner H., Gardner P. S., Mcquillin J. 1970; Respiratory syncytial virus infection of the newborn. British Medical Journal iii:146–147
     [Google Scholar]
  19. Ogilvie M. M., Vathenen A. S., Radford M., Codd J., Key S. 1981; Maternal antibody and respiratory syncytial virus infection in infancy. Journal of Medical Virology 7:263–271
     [Google Scholar]
  20. Pabst H. F., Kreth H. W. 1980; Ontogeny of the immune response as a basis of childhood disease. Journal of Pediatrics 97:519–534
     [Google Scholar]
  21. Parrott R. H., Kim H. W., Arrobio J. O., Hodes D. S., Murphy B. R., Brandt C. D., Camargo E., Chanock R. M. 1973; Epidemiology of respiratory syncytial virus infection in Washington DC. II. Infection and diseases with respect to age, immunologic status, race and sex. American Journal of Epidemiology 98:289–300
     [Google Scholar]
  22. Pringle C. R., Shirodaria P. V., Gimenez H. B., Levine S. 1981; Antigen and polypeptide synthesis by temperature-sensitive mutants of respiratory syncytial virus. Journal of General Virology 54:173–183
     [Google Scholar]
  23. Richardson L. S., Yolken R. H., Belshe R. D., Camargo E., Kim H. W., Chanock R. M. 1978; Enzyme-linked immunoabsorbent assay for measurement of serological response to respiratory syncytial virus infections. Infection and Immunity 20:660–664
     [Google Scholar]
  24. Sims D. G., Downham M. A. P. S., McQuillan J., Gardner P. S. 1976; Respiratory syncytial virus infection in north-east England. British Medical Journal ii:1095–1098
     [Google Scholar]
  25. Sissons J. G. P., Oldstone M. B. A. 1980; Antibody-mediated destruction of virus-infected cells. Advances in Immunology 29:209–260
     [Google Scholar]
  26. Welliver R. C., Kaul T. N., Putnam T. I., Sun M., Riddlesburger K., Ogra P. L. 1980; The antibody response to primary and secondary infection with respiratory syncytial virus: kinetics of class-specific responses. Journal of Pediatrics 96:808–813
     [Google Scholar]
  27. Wright P. F., Belshe R. B., Kim H. W., Van Voris L. P., Chanock R. M. 1982; Administration of a highly attenuated, live respiratory syncytial virus vaccine to adults and children. Infection and Immunity 37:397–400
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-9-1867
Loading
Antibodies to Respiratory Syncytial Virus Polypeptides and their Significance in Human Infection
J. Gen. Virol. 64, 1867 (1983); https://doi.org/10.1099/0022-1317-64-9-1867
/content/journal/jgv/10.1099/0022-1317-64-9-1867
/content/journal/jgv/10.1099/0022-1317-64-9-1867
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error