1887

Abstract

SUMMARY

The possibility that ferret lung macrophages may be one factor operating to prevent infection of susceptible alveolar cells (as demonstrated by organ cultures) by both virulent and attenuated strains of influenza virus has been investigated. Phagocytosis of four strains of influenza virus [A/PR/8/34 (H1N1) and clone 64d (attenuated for ferrets) and clones 64c and 7a (virulent for ferrets) of the recombinant virus A/PR/8/34-A/England/939/69 (H3N2)] by ferrveolar macrophages showed that all strains, whether virulent or attenuated, attached equally well (72 to 93%). Recoveries of virus were similar (17 to 44%) whether phagocytosis occurred at the normal temperature of the ferret (39°C) or at pyrexial temperatures induced during infection (40°C for A/PR/8/34 and clone 64d; 41°C for clones 7a and 64c). Thus, alveolar macrophages probably contribute to the lack of alveolar infection observed .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-8-1807
1983-08-01
2024-11-11
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/8/JV0640081807.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-8-1807&mimeType=html&fmt=ahah

References

  1. Husseini R. H., Sweet C., Bird R. A., Collie M. H., Smith H. 1983; Distribution of viral antigen within the lower respiratory tract of ferrets infected with a virulent influenza virus: production and release of virus from corresponding organ cultures. Journal of General Virology 64:589–598
    [Google Scholar]
  2. Mak N. K., Zhang Y. H., Ada G. L., Tannock G. A. 1982; The humoral and cellular responses of mice to infection with a cold-adapted influenza A virus variant. Infection and Immunity 38:218–225
    [Google Scholar]
  3. Matsuyama T., Sweet C., Collie M. H., Smith H. 1980; Aspects of virulence in ferrets exhibited by influenza virus recombinants of known genetic constitution. Journal of Infectious Diseases 141:351–361
    [Google Scholar]
  4. Mulder J., Hers J. F. Ph 1972 Influenza Groningen: Wolters-Noordhoff;
    [Google Scholar]
  5. Rodgers B. C., Mims C. A. 1981; Interaction of influenza virus with mouse macrophages. Infection and Immunity 31:751–757
    [Google Scholar]
  6. Rodgers B. C., Mims C. A. 1982a; Role of macrophage activation and interferon in the resistance of alveolar macrophages from infected mice to influenza virus. Infection and Immunity 36:1154–1159
    [Google Scholar]
  7. Rodgers B. C., Mims C. A. 1982b; Influenza virus replication in human alveolar macrophages. Journal of Medical Virology 9:177–184
    [Google Scholar]
  8. Sweet C., Stephen J., Smith H. 1974a; Purification of influenza viruses using disulphide-linked immunosorbents derived from rabbit antibody. Immunochemistry 11:295–304
    [Google Scholar]
  9. Sweet C., Stephen I., Smith H. 1974b; The behaviour of antigenically related influenza viruses of differing virulence on disulphide-linked immunosorbents. Immunochemistry 11:823–826
    [Google Scholar]
  10. Sweet C., Bird R. A., Toms G. L., Woodward C. G., Smith H. 1977; Thermal stability and interaction with ferret inflammatory exudates of two clones of influenza virus of differing virulence for both ferrets and man. British Journal of Experimental Pathology 58:635–643
    [Google Scholar]
  11. Sweet C., Macartney J. C., Bird R. A., Cavanagh D., Collie M. H., Husseini R. H., Smith H. 1981; Differential distribution of virus and histological damage in the lower respiratory tract of ferrets infected with influenza viruses of differing virulence. Journal of General Virology 54:103–114
    [Google Scholar]
  12. Wells M. A., Albrecht P., Daniel S., Ennis F. A. 1978; Host defence mechanisms against influenza virus: interaction of influenza virus with murine macrophages in vitro. Infection and Immunity 22:758–762
    [Google Scholar]
  13. Wyde P. R., Wilson M. R., Cate T. R. 1982; Interferon production by leukocytes infiltrating the lungs of mice during primary influenza virus infection. Infection and Immunity 38:1249–1255
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-64-8-1807
Loading
/content/journal/jgv/10.1099/0022-1317-64-8-1807
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error