1887

Abstract

SUMMARY

The surface projections (peplomers) of avian infectious bronchitis virus (IBV) strain M41 have been separated from the nucleocapsid (N) and matrix (M) proteins by sedimentation in a sucrose gradient after virus disruption by the non-ionic detergent Nonidet P40. The peplomers comprised two glycopolypeptides of mol. wt. 90 × 10 (90K; S1) and 84K (S2), shown by analysis of differentially radiolabelled virus to be present in equimolar proportions. Polypeptides of 75K and 110K, which were detected by Coomassie Brilliant Blue staining in similar amounts to S1 and S2 in some unlabelled virus preparations, were absent from peplomer preparations and are probably host cell polypeptides. The S1:S2:N:M polypeptide molar ratio for IBV-M41 was approximately 1:1:6:15.

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/content/journal/jgv/10.1099/0022-1317-64-8-1787
1983-08-01
2021-10-25
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References

  1. Bosshard H. F., Datyner A. 1977; The use of a new reactive dye for quantitation of prestained proteins on polyacrylamide gels. Analytical Biochemistry 82:327–333
    [Google Scholar]
  2. Cavanagh D. 1981; Structural polypeptides of coronavirus IBV. Journal of General Virology 53:93–103
    [Google Scholar]
  3. Cavanagh D. 1983; Coronavirus IBV glycopolypeptides: size of their polypeptide moieties and nature of their oligosaccharides. Journal of General Virology 64:1187–1191
    [Google Scholar]
  4. Collins M. S., Alexander D. J. 1980a; Avian infectious bronchitis virus structural polypeptides: effect of different conditions of disruption and comparison of different strains and isolates. Archives of Virology 63:239–251
    [Google Scholar]
  5. Collins M. S., Alexander D. J. 1980b; The polypeptide composition of isolated surface projections of avian infectious bronchitis virus. Journal of General Virology 48:213–217
    [Google Scholar]
  6. Lanser J. A., Howard C. R. 1980; The polypeptides of infectious bronchitis virus (IBV-41 strain). Journal of General Virology 46:349–361
    [Google Scholar]
  7. Lomniczi B., Morser J. 1981; Polypeptides of infectious bronchitis virus. 1. Polypeptides of the virion. Journal of General Virology 55:155–164
    [Google Scholar]
  8. Macnaughton M. R., Davies H. A. 1980; Two particle types of avian infectious bronchitis virus. Virology 47:365–372
    [Google Scholar]
  9. Macnaughton M. R., Madge M. H. 1977; The polypeptide composition of avian infectious bronchitis virus particles. Archives of Virology 55:47–54
    [Google Scholar]
  10. Macnaughton M. R., Madge M. H., Davies H. A., Dourmashkin R. R. 1977; Polypeptides of the surface projections and the ribonucleoprotein of avian infectious bronchitis virus. Journal of Virology 24:821–825
    [Google Scholar]
  11. Nagy E., Lomniczi B. 1979; Polypeptide patterns of infectious bronchitis virus serotypes fall into two categories. Archives of Virology 61:341–345
    [Google Scholar]
  12. Siddell S., Wege H., Ter Meulen V. 1982; The structure and replication of coronaviruses. Current Topics in Microbiology and Immunology 99:131–163
    [Google Scholar]
  13. Stern D. F., Sefton B. M. 1982; Coronavirus proteins: structure and function of the oligosaccharides of the avian infectious bronchitis virus glycoproteins. Journal of Virology 44:804–812
    [Google Scholar]
  14. Stern D. F., Burgess L., Sefton B. M. 1982; Structural analysis of virion proteins of the avian coronavirus infectious bronchitis virus. Journal of Virology 42:208–219
    [Google Scholar]
  15. Sturman L. S., Holmes K. V., Behnke J. 1980; Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid. Journal of Virology 33:449–462
    [Google Scholar]
  16. Wadey C. N., Westaway E. G. 1981; Structural proteins and glycoproteins of infectious bronchitis virus particles labeled during growth in chick embryo cells. Intervirology 15:19–27
    [Google Scholar]
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