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The addition of actinomycin D (25 µg/ml) or cordycepin (1 mm) to protoplast cultures immediately after inoculation with particles of tobacco mosaic, tobacco rattle, tobacco ringspot or potato leafroll viruses resulted in a decrease in the proportion of protoplasts becoming infected, as judged by staining with fluorescent antibody to virus particles. A delay of a few hours between the inoculation and the addition of either inhibitor largely or completely eliminated this effect. In contrast, infection was unaffected by the addition of actinomycin D when the protoplasts were inoculated with RNA preparations from tobacco mosaic or tobacco rattle viruses.
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