1887

Abstract

SUMMARY

Complementary DNA (cDNA) of citrus tristeza virus (CTV) RNA, synthesized using calf thymus DNA random primers, was converted to a double-stranded form and inserted into the I site of the pBR322 plasmid by the G-C tailing method. Bacterial clones harbouring virus-specific sequences were detected by colony hybridization with a P-labelled viral RNA probe. Hybridization patterns of denatured virus RNA revealed the presence of three types of specific clones: those hybridizing with a distinct narrow band corresponding to the full-length virus RNA, those hybridizing with a broader band of virus RNA sequences, and those hybridizing with several distinct virus-related RNA bands. Similar patterns were obtained when these clones were hybridized to purified double-stranded RNA from CTV-infected plants. None of these cDNA clones hybridized with similarly treated preparations extracted from healthy plants. The origin of variation among the CTV clones is discussed.

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1983-08-01
2024-12-10
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References

  1. Bar-Joseph M., Hull R. 1974; Purification and partial characterization of sugar beet yellows virus. Virology 62:552–562
    [Google Scholar]
  2. Bar-Joseph M., Loebenstein G., Cohen J. 1970; Partial purifications of virus-like particles associated with citrus tristeza disease. Phytopathology 60:75–78
    [Google Scholar]
  3. Bar-Joseph M., Garnsey S. M., Gonsalves D. 1979a; The closteroviruses: a distinct group of elongated plant viruses. Advances in Virus Research 25:93–168
    [Google Scholar]
  4. Bar-Joseph M., Garnsey S. M., Gonsalvales D., Moscovitz M., Purcifull D. E., Clark M. F., Loebenstein G. 1979b; The use of enzyme-linked immunosorbent assay for detection of citrus tristeza virus. Phytopathology 69:190–194
    [Google Scholar]
  5. Bar-Joseph M., Rosner A., Moscovitz M., Hull R. 1983; A simple procedure for the extraction of doublestranded RNA from virus-infected plants. Journal of Virological Methods 6:1–8
    [Google Scholar]
  6. Dawson W. O., Dodds J. A. 1982; Characterization of subgenomic double stranded RNAs from virus-infected plants. Biochemical and Biophysical Research Communications 107:1230–1235
    [Google Scholar]
  7. Dodds J. A., Bar-Joseph M. 1983; Double-stranded RNA from plants infected with closteroviruses. Phytopathology 73:419–423
    [Google Scholar]
  8. Fellous A., Ginzburg I., Littauer U. Z. 1982; Modulation of tubulin mRNA levels by interferon in human lymphoblastoid cells. EMBO Journal 1:835–839
    [Google Scholar]
  9. Garnsey S. M., Gonsalves D., Purcifull D. E. 1977; Mechanical transmission of citrus tristeza virus. Phytopathology 67:956–968
    [Google Scholar]
  10. Ginsberg I., De Baetseiler A., Walker M. D., Behar L., Frischant A. M., Littauer U. Z. 1980; Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids. Nucleic Acids Research 8:3553–3564
    [Google Scholar]
  11. Goelet P., Karn J. 1982; Tobacco mosaic virus induces the synthesis of a family of 3′ coterminal messenger RNAs and their complements. Journal of Molecular Biology 154:541–550
    [Google Scholar]
  12. Goelet P., Lomonossoff E. P., Butler P. J. G., Akam M. E., Gait M. J., Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, U. S. A 79:5818–5822
    [Google Scholar]
  13. Gonda T. J., Symons R. H. 1978; The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: its application to several strains of cucumoviruses. Virology 88:361–370
    [Google Scholar]
  14. Gonsalves D., Purcifull D. E., Garnsey S. M. 1978; Purification and serology of citrus tristeza virus. Phytopathology 68:553–559
    [Google Scholar]
  15. Gould A. R., Palukaitis P., Symons R. H., Mossop D. 1978; Characterization of satellite RNA associated with cucumber mosaic virus. Virology 84:443–455
    [Google Scholar]
  16. Gumpf J. D., Bar-Joseph M., Dodds J. A. 1981; Purification of citrus tristeza virus on sucrose-cesium sulphate cushion gradients and estimation of its RNA size. Phytopathology 71:878
    [Google Scholar]
  17. Kiefer M. C., Daubert S. D., Schneider I. R., Bruening G. 1982; Multimeric forms of satellite of tobacco ring-spot virus RNA. Virology 121:262–273
    [Google Scholar]
  18. Kitajima E. W., Silva D. M., Oliveira A. R., Müller G. W., Costa A. S. 1964; Electron microscopical investigations of tristeza. In Proceedings of the 3rd Conference of the International Organization of Citrus Virologists pp 1–9 Edited by Price W. E. Gainesville: University of Florida Press;
    [Google Scholar]
  19. Kummert J. 1980; Synthesis and characterization of DNA complementary to carnation mottle virus RNA. Virology 105:35–40
    [Google Scholar]
  20. Maizels N. 1976; Dictyostelium 17S, 25S and 5s-r DNAs lie within a 38, 000 base pair repeated unit. Cell 9:431–438
    [Google Scholar]
  21. Maule A. J., Hull R., Donson J. 1983; The application of spot hybridization to the detection of DNA and RNA viruses in plant tissues. Journal of Virological Methods 6: (in press)
    [Google Scholar]
  22. Meshi I., Takamatsu N., Ohno T., Okada Y. 1982; Molecular cloning of the complementary DNA copies of the common and cowpea strains of tobacco mosaic virus RNA. Virology 118:64–75
    [Google Scholar]
  23. Morris T. J., Dodds J. A. 1979; Isolation and analysis of double-stranded RNA from virus-infected plants and fungal tissue. Phytopathology 69:854–858
    [Google Scholar]
  24. Mossop D. W., Francki R. I. B. 1979; Comparative studies on two satellite RNAs of cucumber mosaic virus. Virology 95:395–404
    [Google Scholar]
  25. Nagel J., Hiebert E., Lee R. F. 1982; Citrus tristeza virus RNA translated with a rabbit reticulocyte lysate: capsid protein identified as one of the products. Phytopathology 72:953
    [Google Scholar]
  26. Palukaitis P., Symons R. H. 1980; Nucleotide sequence homology of thirteen tobacco virus RNAs as determined by hybridization analysis with complementary DNA. Virology 107:354–361
    [Google Scholar]
  27. Rosner A., Bar-Joseph M., Moscovitz M., Mevarech M. 1983; Diagnosis of specific viral RNA sequences in plant extracts by hybridization with a polynucleotide kinase mediated 32P-labeled double-stranded RNA probe. Phytopathology 73: (in press)
    [Google Scholar]
  28. Talianski M. E., Boykov S. V., Molishenko S. I., Kasvan V. M., Atabekov J. G. 1979; A study of barley stripe mosaic virus (BSMV) genome. I. Determination of sequence homology between BSMV RNA species. Molecular and General Genetics 175:89–92
    [Google Scholar]
  29. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:324–330
    [Google Scholar]
  30. Van De Valle M. J., Siegel A. 1982; Relationships between strains of tobacco mosaic virus and other selected plant viruses. Phytopathology 72:390–395
    [Google Scholar]
  31. Wickens M. P., Buell G. N., Schimke R. T. 1978; Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid and ovalbumin mRNA. Journal of Biological Chemistry 253:2483–2495
    [Google Scholar]
  32. Zelcer A., Weaber K. F., Balazs E., Zaitlin M. 1981; The detection and characteristics of viral related doublestranded RNAs in tobacco mosaic virus infected plants. Virology 113:417–427
    [Google Scholar]
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