It has been shown previously, by sequence analysis of the S RNA segment of snowshoe hare (SSH) bunyavirus, that two overlapping open reading frames in the viral complementary sequence code for proteins with molecular weights of 26.8 × 10 and 10.5 × 10 respectively. In addition to the viral nucleocapsid (N) protein, which is coded by the S RNA, analyses of parental and reassortant bunyavirus-infected cell extracts have shown that the viral S RNA and M RNA species each code for nonstructural proteins (NS and NS, respectively). In the present report, translation analyses of the S mRNA species recovered from virus-infected cells indicate that a single size class of mRNA directs the synthesis of N and NS. Compositional analyses of selected tryptic peptides of N and NS have provided proof that N is the product of the first open reading frame, and NS the product of the second.


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