1887

Abstract

SUMMARY

By the use of horseradish peroxidase-labelled Fab fragments of monoclonal antibodies, five major structural components of Sendai virus, namely the nucleocapsid (NP), polymerase (P), matrix (M), fusion (F), and haemagglutinin—neuraminidase (HN) proteins were localized in infected Vero cells. The P and NP proteins were found in association with large clusters of ribosome-like particles and nucleocapsids in the cell cytoplasm. They were not concentrated at the cytoplasmic membrane, except in nucleocapsids within budding viral particles. F and HN proteins, on the other hand, were found in connection with ribosomes and endoplasmic reticulum in the cell cytoplasm, but not in nucleocapsids. Both proteins were evenly distributed on the outer cytoplasmic membrane and appeared on the surface clearly before budding of viral particles. The M protein was seen in connection with both nucleocapsids and ribosomes. It was not found at the cell surface except in budding viral particles.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-8-1673
1983-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/8/JV0640081673.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-8-1673&mimeType=html&fmt=ahah

References

  1. Boulan E. R., Pendergast M. 1980; Polarized distribution of viral envelope proteins in the plasma membrane of infected epithelial cells. Cell 20:45–54
    [Google Scholar]
  2. Bowen H. A., Lyles D. S. 1982; Kinetics of incorporation of Sendai virus proteins into host plasma membranes and virions. Virology 121:1–11
    [Google Scholar]
  3. Hay A. J. 1974; Studies on the formation of the influenza virus envelope. Virology 60:398–418
    [Google Scholar]
  4. Howe C., Morgan C., De Vauxstcyr C., Hsu K. C., Rose H. M. 1967; Morphogenesis of type 2 parainfluenza virus examined by light and electron microscopy. Journal of Virology 1:215–237
    [Google Scholar]
  5. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  6. Lamb R. A., Choppin P. W. 1977; The synthesis of Sendai virus polypeptides in infected cells. Virology 81:371–381
    [Google Scholar]
  7. Lin C. T., Chang J., Chen J. P. 1975; Ultrastructural localisation of gamma chain and immunoglobulin G in human lymphocytes using enzyme labelled Fab fragments. Journal of Histochemistry and Cytochemistry 23:624–631
    [Google Scholar]
  8. Nagai Y., Ogura H., Klenk H.-D. 1976; Studies on the assembly of the envelope of Newcastle disease virus. Virology 69:523–538
    [Google Scholar]
  9. Norrby E., Chen S.-E., Togashi T., Shesberadaran H., Johnson K. P. 1982; Five measles virus antigens demonstrated by use of mouse hybridoma antibodies in productively infected tissue culture cells. Archives of Virology 71:1–11
    [Google Scholar]
  10. Örvell C., Grandien M. 1982; The effects of monoclonal antibodies on biological activities of structural proteins of Sendai virus. Journal of Immunology 129:2779–2787
    [Google Scholar]
  11. Portner A., Kingsbury D. W. 1976; Regulatory events in the synthesis of Sendai virus polypeptides and their assembly into virions. Virology 73:79–88
    [Google Scholar]
  12. Schwalbe J. C., Hightower L. E. 1982; Maturation of the envelope glycoproteins of Newcastle disease virus on cellular membranes. Journal of Virology 41:947–957
    [Google Scholar]
  13. Wilson M. B., Nakane P. K. 1978; Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In Immunofluorescence and Related Staining Techniques pp 215–224 Edited by Knapp W., Holubar K., Wiek G. Amsterdam: Elsevier;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-8-1673
Loading
/content/journal/jgv/10.1099/0022-1317-64-8-1673
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error