1887

Abstract

SUMMARY

Sonchus yellow net virus (SYNV) contains approximately 18% lipid composed of 62% phospholipids, 31% sterols and 7% triglycerides. The polar phospholipids consist of phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine, phosphatidyl inositol and phosphatidyl glycerol respectively. The fatty acid composition of the individual phospholipids includes various ratios of palmitic, stearic, oleic, linoleic and linolenic acids. The neutral lipid fraction is composed primarily of free sterols, sterol esters and triglycerides. Sterol glycosides are also present in the polar lipid fraction. Stigmasterol, β-sitosterol, cholesterol and campesterol represent about 50%, 22%, 20% and 8% respectively, by weight, of the total sterol, and both glycosylated and nonglycosylated derivatives are present. Galactolipids are not detectable in the SYNV preparations, although small amounts of other unidentified lipids are present. This study suggests that a large number of selected lipids are obtained from the host during assembly of virus particles.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-7-1607
1983-07-01
2022-11-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/7/JV0640071607.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-7-1607&mimeType=html&fmt=ahah

References

  1. Ahmed M. E., Black L. M., Perkins E. G., Walker B. L., Kummerow F. A. 1964; Lipid in potato yellow dwarf virus. Biochemical and Biophysical Research Communications 17:103–107
    [Google Scholar]
  2. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  3. Christie S. R., Hall D. W. 1979; A new hybrid species of Nicotiana (Solanaceae). Baileya 20:133–136
    [Google Scholar]
  4. COMpans R. W., Klenk H. D. 1979; Viral membranes. In Comprehensive Virology vol 13: pp 293–407 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  5. David A. E. 1977; Assembly of the vesicular stomatitis virus envelope: transfer of viral polypeptides from polysomes to cellular membranes. Virology 76:98–108
    [Google Scholar]
  6. Diringer H., Kulas H. P., Schneider L. G., Schlumberger H. D. 1973; The lipid composition of rabies virus. Zeitschrift für Naturforschung 28c:90–93
    [Google Scholar]
  7. Douce R., Joyard J. 1980a; Chloroplast envelope lipids: detection and biosynthesis. Methods in Enzfmology 69:290–301
    [Google Scholar]
  8. Douce R., Joyard J. 1980b; Plant galactolipids. In The Biochemistry of Plants, a Comprehensive Treatise vol 4: pp 321–362 Edited by Stumpf P. K. New York & London: Academic Press;
    [Google Scholar]
  9. Duperon R., Brillard M., Duperon P. 1972; Localisation intracellulaire des differents composes steroliques dans le tubercule de Pomme de terre, avant sa germination. Compte rendu hebdomadaire des seances de I’Academie des sciences, serie D 274:2321–2324
    [Google Scholar]
  10. Francki R. I. B., Randles J. W. 1980; Rhabdoviruses infecting plants. In Rhabdoviruses vol 3: pp 135–165 Edited by Bishop D. H. L. Florida: CRC Press;
    [Google Scholar]
  11. Franke W. W. 1974; Structure, biochemistry and functions of the nuclear envelope. In International Review of Cytology, Supplement 4: Aspects of Nuclear Structure and Function pp 71–236 Edited by Bourne G. H., Danielli J. F., Leon K. W. New York & London: Academic Press;
    [Google Scholar]
  12. Grunwald C. 1975; Phytosterols in tobacco leaves at various stages of physiological maturity. Phytochemistry 14:79–82
    [Google Scholar]
  13. Harwood J. L. 1980; Plant acyl lipids: structure, distribution and analysis. In The Biochemistry of Plants, a Comprehensive Treatise vol 4: pp 1–55 Edited by Stumpf P. K. New York & London: Academic Press;
    [Google Scholar]
  14. Hull R. 1970; Large RNA plant-infecting viruses. In The Biology of Large RNA Viruses pp 153–164 Edited by Barry R. D., Mahy B. W. J. New York & London: Academic Press;
    [Google Scholar]
  15. Jackson A. O. 1978; Partial characterization of the structural proteins of sonchus yellow net virus. Virology 87:172–181
    [Google Scholar]
  16. Jackson A. O., Christie S. R. 1977; Purification and some physicochemical properties of sonchus yellow net virus. Virology 77:344–355
    [Google Scholar]
  17. Jackson A. O., Milbrath G. M., Jedlinski H. 1981; Rhabdoviruses of the Gramineae. In Virus and Virus-tike Diseases of Maize in the United States pp 51–76 Southern Cooperative Series Bulletin 247 Edited by Gordon D. T., Knoke J. K., Scott G. E. Wooster, Ohio: Ohio Agricultural Research and Development Center;
    [Google Scholar]
  18. Karunen P., Mikola H., Ekman R. 1980; Occurrence of steryl and wax esters in Dicoranum elongatum. Physiologia plantarum 48:554–559
    [Google Scholar]
  19. Kemp R. J., Mercer E. J. 1968; Studies on the sterols and sterol esters of the intracellular organelles of maize shoots. Biochemical Journal 110:119–125
    [Google Scholar]
  20. Khan A., Rilfors L., Wieslander A., Lindblom G. 1981; The effect of cholesterol on the phase structure of glucolipids from Acholeplasma laidlawii membranes. European Journal of Biochemistry 116:215–220
    [Google Scholar]
  21. McSharry J. J., Wagner R. R. 1971; Lipid composition of purified vesicular stomatitis viruses. Journal of Virology 7:59–70
    [Google Scholar]
  22. Martelli G. P., Russo M. 1977; Rhabdoviruses of plants. In The Atlas of Insect and Plant Viruses pp 181–213 Edited by Maramorosch K. New York & London: Academic Press;
    [Google Scholar]
  23. Mazliak P. 1977; Glyco- and phospholipids of biomembranes in higher plants. In Lipids and Lipid Polymers in Higher Plants pp 48–74 Edited by Tevini M., Lichtenthaler H. New York: Springer-Verlag;
    [Google Scholar]
  24. Morrison T. G., Mcquain C. O. 1978; Assembly of viral membranes: nature of the association of vesicular stomatitis virus proteins to membranes. Journal of Virology 26:115–125
    [Google Scholar]
  25. Morrison W. R., Smith L. M. 1964; Preparation of fatty acid methyl esters and dimethylacetates from lipids with boronfluoridemethanol. Journal of Lipid Research 5:600–608
    [Google Scholar]
  26. Nichols B. W. 1964; Separation of plant phospholipids and glycolipids. In New Biochemical Separations pp 321–337 Edited by James A. T., Morris L. J. London: Van Nostrand Reinhold;
    [Google Scholar]
  27. Nichols B. W., Harris R. V., James A. T. 1965; The lipid metabolism of blue-green algae. Biochemical and Biophysical Research Communications 20:256–262
    [Google Scholar]
  28. Patzer E. J., Moore N. F., Barenholz Y., Shaw J. M., Wagner R. R. 1978; Lipid organization of the membrane of vesicular stomatitis virus. Journal of Biological Chemistry 253:4544–4550
    [Google Scholar]
  29. Philipp E. L., Franke W. W., Keenan T. W., Stadler J., Jarasch E. D. 1976; Characterization of nuclear membranes and endoplasmic reticulum isolated from plant tissue. Journal of Cell Biology 68:11–29
    [Google Scholar]
  30. Schlesinger H. R., Wells H. J., Hummeler K. 1973; Comparison of the lipids of intracellular and extracellular rabies viruses. Journal of Virology 12:1028–1030
    [Google Scholar]
  31. Sellden G., Selstam E. 1976; Changes in chloroplast lipids during the development of photosynthesis activity in barley etiochloroplasts. Physiologia plantarum 37:35–41
    [Google Scholar]
  32. Shaw N. 1968; The detection of lipids on thin-layer chromatograms with the periodate-SchifTs reagent. Biochimica et biophysica acta 164:435–436
    [Google Scholar]
  33. Sinha R. C., Harwalkar V. R., Behki R. M. 1976; Chemical composition and some properties of wheat striate mosaic virus. Phytopathologische Zeitschrift 87:314–323
    [Google Scholar]
  34. Spector T. 1978; Refinement of the Coomassie Blue method of protein quantitation: a simple and linear spectrophotometric assay for 0.5 to 50 µg of protein. Analytical Biochemistry 36:142–146
    [Google Scholar]
  35. Toriyama S. 1972; Purification and some properties of northern cereal mosaic virus. Virus 22:8–18
    [Google Scholar]
  36. Toriyama S. 1976; Sterol composition of northern cereal mosaic virus. Annals of the Phytopathological Society of Japan 42:494–496
    [Google Scholar]
  37. Trevathan L. E., Moore L. D., Orcutt D. M. 1979; Symptom expression and free sterol and fatty acid composition of flue-cured tobacco plants exposed to ozone. Phytopathology 69:582–585
    [Google Scholar]
  38. Vaskovsky V. E., Kostetsky E. Y. 1968; Modified spray for the detection of phospholipids on thin layer chromatograms. Journal of Lipid Research 9:396
    [Google Scholar]
  39. Wagner H., Horhammar L., Wolff P. 1961; Dunnschichtchromatography von Phosphatiden und Glykolipiden. Biochemische Zeitschrift 334:175–184
    [Google Scholar]
  40. White J., Helenius A. 1980; pH-dependent fusion between the Semliki Forest virus membrane and liposomes. Proceedings of the National Academy of Sciences, U. S. A 77:3273–3277
    [Google Scholar]
  41. Wieslander A., Christiansson A., Rilfors L., Lindblom G. 1980; Lipid bilayer stability in membranes. Regulation of lipid composition of Acholesplasma laidlawii as governed by molecular shape. Biochemistry 19:3650–3655
    [Google Scholar]
  42. Willemot C. 1980; Sterols in hardening winter wheat. Phytochemistry 19:1071–1073
    [Google Scholar]
  43. Zakowski J. J., Petri W. A., Wagner R. R. 1981; Role of matrix protein in assembling the membrane of vesicular stomatitis virus: reconstruction of matrix protein with negatively charged phospholipid vesicles. Biochemistry 20:3902–3907
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-7-1607
Loading
/content/journal/jgv/10.1099/0022-1317-64-7-1607
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error