1887

Abstract

SUMMARY

Two distinct antiviral activities can be detected in L cells treated with low levels of interferon and infected with a one-step interferon-sensitive mutant of mengovirus (-1). The first antiviral activity (AVA-1) primarily delayed virus RNA and protein synthesis and thereby lengthened the virus replication cycle. It did not prevent cell death. The second antiviral activity (AVA-2) allowed the virus-induced inhibition of host macromolecular synthesis but inhibited all other virus functions. By 9 to 12 h post-infection host synthesis resumed and most cells survived. The data suggest that some step in the virus replication cycle activates AVA-2 leading to the destruction of the virus genome 6 to 12 h after infection. In unprotected cells the yields of parental virus ( ) and -1 were similar. No qualitative or quantitative differences in virus products were observed by several techniques. The -1 virus seems to have lost a wild-type function which normally blocks the action of AVA-2.

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1983-07-01
2022-08-14
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References

  1. Allen O., Fantes K. H. 1980; A family of structural genes for human lymphoblastoid (leukocyte-type) interferon. Nature, London 287:408–411
    [Google Scholar]
  2. Alonso M. A., Carrasco L. 1981; Reversion by hypotonic medium of the shutoff of protein synthesis induced by encephalomyocarditis virus. Journal of Virology 37:535–540
    [Google Scholar]
  3. Baglioni C., Nilsen T. W. 1981; The action of interferon at the molecular level. American Scientist 69:392–399
    [Google Scholar]
  4. Baglioni C., Maroney P. A., West D. K. 1979; 2′5′ oligo(A) polymerase activity and inhibition of viral RNA synthesis in interferon-treated HeLa cells. Biochemistry 18:1765–1770
    [Google Scholar]
  5. Ball L. A. 1979; Induction of 2′5′-oligoadenylate synthetase activity and a new protein by chick interferon. Virology 94:282–296
    [Google Scholar]
  6. Chang T. T., Simon E. H., Fleischmann W. R. Jun 1973; The mechanism of interferon action in single cells: accumulation of intracellular virus. Journal of General Virology 20:139–49
    [Google Scholar]
  7. Chernajovsky Y., Kimchi A., Schmidt A., Zilberstein A., Revel M. 1979; Differential effects of two interferon-induced translational inhibitors on initiation of protein synthesis. European Journal of Biochemistry 96:35–41
    [Google Scholar]
  8. Collins F. D., Roberts W. K. 1972; Mechanism of mengovirus-induced cell injury in L cells: use of inhibitors of protein synthesis to dissociate virus-specific events. Journal of Virology 10:969–978
    [Google Scholar]
  9. Fleischmann W. R. Jun, Simon E. H. 1973; Effect of interferon on virus production from isolated single cells. Journal of General Virology 20:127–137
    [Google Scholar]
  10. Fout G. S. 1980 Studies on an interferon sensitive mutant of mengovirus Ph.D. thesis Purdue University;
    [Google Scholar]
  11. Fout G. S., Simon E. H. 1981; Studies on an interferon-sensitive mutant of mengovirus: effects on host RNA and protein synthesis. Journal of General Virology 52:391–394
    [Google Scholar]
  12. Haase A. T., Baron S., Levy H., Kasel J. A. 1969; Mengovirus-induced cytopathic effect in L cells: protective effect of interferon. Journal of Virology 4:490–495
    [Google Scholar]
  13. Helentjaris T., Ehrenfeld E., Brown-Luedi M. L., Hershey J. W. B. 1979; Alterations in initiation factor activity from poliovirus infected cells. Journal of Biological Chemistry 254:10973–10978
    [Google Scholar]
  14. Hovanessian A. G., Meurs E., Montagnier L. 1981; Lack of systematic correlation between the interferon mediated antiviral state and the levels of 2-5A synthetase and protein kinase in three different types of murine cells. Journal of Interferon Research 1:179–190
    [Google Scholar]
  15. Ito Y., Nishiyama Y., Shimokata K., Nagata I., Kunii A. 1978; Temperature sensitivity of interferon susceptibility in L cells persistently infected with hemagglutinating virus of Japan (HVJ). Virology 89:342–345
    [Google Scholar]
  16. Jen G., Detjen B. M., Thach R. E. 1980; Shutoff of HeLa cell protein synthesis by encephalomyocarditis virus and poliovirus: a comparative study. Journal of Virology 35:150–156
    [Google Scholar]
  17. Kimchi A., Shulman L., Schmidt A., Chernajovsky Y., Fradin A., Revel M. 1979; Kinetics of the induction of three translation-regulatory enzymes by interferon. Proceedings of the National Academy of Sciences, U. S. A 76:3208–3212
    [Google Scholar]
  18. Mccutchan J. H., Pagano J. S. 1968; Enhancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran. Journal of the National Cancer Institute 41:351–357
    [Google Scholar]
  19. Maizel J. V. Jr 1971; Polyacrylamide gel electrophoresis of viral proteins. Methods in Virology 5:180–247
    [Google Scholar]
  20. Marcus P. I., Terry T. M., Levine S. 1975; Interferon action. II. Membrane-bound alkaline ribonuclease activity in chick embryo cells manifesting interferon-mediated interference. Proceedings of the National Academy of Sciences, U. S. A 72:182–186
    [Google Scholar]
  21. Marcus P. I., Guidon P. T. Jr, Sekellick M. J. 1981; Interferon induction by viruses. VII. Mengovirus: ‘interferon-sensitive’ mutant phenotype attributed to interferon-inducing particle activity. Journal of Interferon Research 1:601–611
    [Google Scholar]
  22. Meurs E., Hovanessian A. G., Montagnier L. 1981; Interferon-mediated antiviral state in human MRC5 cells in the absence of detectable levels of 2-5A synthetase and protein kinase. Journal of Interferon Research 1:219–232
    [Google Scholar]
  23. Nagata S., Mantei N., Weismann C. 1980; The structure of one of the eight or more distinct chromosomal genes for human interferon-a. Nature, London 287:401–408
    [Google Scholar]
  24. Nilsen T. W., Wood D. L., Baglioni C. 1980; Virus-specific effects of interferon in embryonal carcinoma cells. Nature, London 286:178–180
    [Google Scholar]
  25. Penman S., Summers D. 1965; Effects on host cell metabolism following synchronous infection with poliovirus. Virology 27:614–620
    [Google Scholar]
  26. Ramabhadran T. V., Thach R. E. 1981; Translational elongation rate changes in encephalomyocarditis virus-infected and interferon-treated cells. Journal of Virology 39:573–583
    [Google Scholar]
  27. Rechcigl M. Jr (editor) 1971; Intracellular protein turnover and the roles of synthesis and degradation in regulation of enzyme levels. In Enzyme Synthesis and Degradation in Mammalian Systems pp 236–310 Basel: S. Karger;
    [Google Scholar]
  28. Sakaki Y. 1974; Inactivation of the ATP-dependent DNase of E. coli after infection with double stranded DNA phages. Journal of Virology 14:1611–1612
    [Google Scholar]
  29. Samuel C. E. 1979; Mechanism of interferon action. Kinetics of interferon action in mouse cells: phosphorylation of protein synthesis initiation factor eIF-2 and ribosome-associated protein PI. Virology 93:281–285
    [Google Scholar]
  30. Simon E., Isono N. 1982; Studies on the mode of action of is-1, an interferon-sensitive mutant of mengovirus. American Society for Microbiology Abstract T 25
    [Google Scholar]
  31. Simon E. H., Rung S., Koh T. T., Brandman P. 1976; Interferon-sensitive mutants of mengovirus. I. Isolation and biological characterization. Virology 69:727–736
    [Google Scholar]
  32. Stebbing N. 1979; Protection of mice against infection with wild-type mengovirus and an interferon-sensitive mutant (is-1) by polynucleotides and interferons. Journal of General Virology 44:255–260
    [Google Scholar]
  33. Stitz L., Schellekens H. 1980; Influence of input multiplicity of infection on the antiviral activity of interferon. Journal of General Virology 46:205–210
    [Google Scholar]
  34. Tamm I., Sehgal P. B. 1979; New evidence for regulated transcription of hnRNA and for regulated translation of interferon mRNA in human cells. In Specific Eukaryotic Genes pp 424–441 Edited by Engbert J., Klenow H., Leick V. Copenhagen: Munskgaard;
    [Google Scholar]
  35. Taniguchi T., Mantei N., Schwarzstein M., Nagata S., Muramatsu M., Weismann C. 1980; Human leukocyte and fibroblast interferons are structurally related. Nature, London 285:547–549
    [Google Scholar]
  36. Thacore H., Youngner J. S. 1973; Rescue of VSV from interferon-induced resistance by superinfection with vaccina virus. II. Effect of UV-inactivated vaccina and metabolic inhibitors. Virology 56:512–522
    [Google Scholar]
  37. Trachsel H., Sonnenberg N., Shatkin A. H., Rose J. K., Leong K., Bergmann J. E., Gordon J., Baltimore D. 1980; Purification of a factor that restores translation of vesicular stomatitis virus messenger RNA in extracts from poliovirus-infected HeLa cells. Proceedings of the National Academy of Sciences, U. S. A 77:770–774
    [Google Scholar]
  38. Vaquero C., Aujean-Rigaud O., Sanceau J., Falcoff R. 1981; Effect of interferon on transient shut-off of cellular RNA and protein synthesis induced by mengovirus infection. Antiviral Research 1:123–134
    [Google Scholar]
  39. Williams B. R. G., Kerr L. M., Gilbert C. S., White C. N., Ball L. A. 1978; Synthesis and breakdown of pppA2′p5′A2′p5′A and transient inhibition of protein synthesis in extracts from interferon-treated and control cells. European Journal of Biochemistry 92:455–462
    [Google Scholar]
  40. Williams J. G., Radding L. M. 1981; Partial purification and properties of an exonuclease inhibitor induced by bacteriophage Mu-1. Journal of Virology 39:548–558
    [Google Scholar]
  41. Ziola B. R., Scraba D. G. 1976; Structure of the mengo virion. IV. Amino- and carboxyl-terminal analysis of the major capsid polypeptides. Virology 71:111–121
    [Google Scholar]
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