1887

Abstract

SUMMARY

A human papillomavirus type 1 (HPV-1)/pBR322 recombinant plasmid was constructed consisting of two complete HPV-1 genomes in a head-to-tail arrangement, inserted into the HI site of pBR322. To obtain established human keratinocytes carrying dimeric HPV-1, origin-minus simian virus 40 (SV40) DNA was used as a dominant transforming marker in co-transfection experiments performed with cultured human foetal keratinocytes. Using the Southern blotting technique, HPV-1 DNA was detected in one out of five SV40-transformed human keratinocyte cell lines which were obtained in this way. Further blotting experiments using SV40, pBR322 and HPV-1 DNA as probes revealed patterns of hybridization which were consistent with co-integration of SV40 DNA with between two and four copies of the HPV-1 genome. The HPV-1 sequences in this cell line were virtually non-methylated and were transcribed at a lower level than SV40 mRNAs in the same cultured cells. A low level of monomeric HPV form I DNA was detected by blotting of undigested total cellular DNA. No evidence for a stimulation of form I HPV DNA replication could be obtained by blotting analysis of total DNA extracted from keratinizing cysts, which were formed by subcutaneous inoculation of these cells into nude mice.

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/content/journal/jgv/10.1099/0022-1317-64-7-1509
1983-07-01
2022-01-18
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References

  1. Amtmann E., Muller H., Sauer G. 1980; Equine connective tissue tumors contain unintegrated bovine papilloma virus DNA. Journal of Virology 35:962–964
    [Google Scholar]
  2. Basilico C., Zouzias D., Della-Valle G., Gattoni S., Colankuoni V., Fenton R., Dailey L. 1979; Integration and excision of polyoma virus genomes. Cold Spring Harbor Symposia on Quantitative Biology 44:611–620
    [Google Scholar]
  3. Danos O., Katinka M., Yaniv M. 1980; Molecular cloning, refined physical map and heterogeneity of methylation sites of papilloma virus type la DNA. European Journal of Biochemistry 109:457–461
    [Google Scholar]
  4. Danos O., Katinka M., Yaniv M. 1982; Human papillomavirus la complete DNA sequence: a novel type of genome organization among Papovaviridae. EMBO Journal 1:231–236
    [Google Scholar]
  5. Doran T. I., Vidrich A., Sun T.T. 1980; Intrinsic and extrinsic regulation of the differentiation of skin, corneal and esophageal epithelial cells. Cell 22:17–25
    [Google Scholar]
  6. Fried M., Klein B., Murray K., Greenway P., Tooze J., Boll W., Weissman C. 1979; InfeCtivity in mouse fibroblasts of polyoma DNA integrated into plasmid pBR322 or lambdoid phage DNA. Nature, London 279:811–816
    [Google Scholar]
  7. Gissmann L., Pfister H., Zur Hausen H. 1977; Human papilloma viruses (HPV): characterization of four different isolates. Virology 76:569–580
    [Google Scholar]
  8. Gluzman Y., Frisque F. J., Sambrook J. 1979; Origin-defective mutants of SV40. Cold Spring Harbor Symposia on Quantitative Biology 44:293–300
    [Google Scholar]
  9. Goodman H. M., Macdonald R. J. 1979; Cloning of hormone genes from a mixture of cDNA molecules. Methods in Enzymology 68:75–90
    [Google Scholar]
  10. Graham F. L., Van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  11. Green H. 1978; Cyclic AMP in relation to proliferation of the epidermal cell: a new view. Cell 15:801–811
    [Google Scholar]
  12. Jenson A. B., Rosenthal J. D., Olson C., Pass F., Lancaster W. D., Shah K. 1980; Immunologic relatedness of papillomaviruses from different species. Journal of the National Cancer Institute 64:495–500
    [Google Scholar]
  13. La Porta R. F., Taichman L. B. 1982; Human papilloma viral DNA replicates as a stable episome in cultured epidermal keratinocytes. Proceedings of the National Academy of Sciences, U. S. A 79:3393–3397
    [Google Scholar]
  14. Lancaster W. D. 1981; Apparent lack of integration of bovine papillomavirus DNA in virus-induced equine and bovine tumor cells and virus-transformed mouse cells. Virology 108:251–255
    [Google Scholar]
  15. Lania L., Boast S., Fried M. 1982; Excision of polyoma virus genomes from chromosomal DNA by homologous recombination. Nature, London 295:349–350
    [Google Scholar]
  16. Law M. F., Lowy D. R., Dvoretzky I., Howley P. M. 1981; Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences. Proceedings of the National Academy oj Sciences, U. S. A 78:2727–2731
    [Google Scholar]
  17. Maniatis T., Fritsch E. F., Sambrook J. 1982; Extraction, purification and analysis of mRNA from eukaryotic cells. In Molecular Cloning - A Laboratory Manual p 202 New York: Cold Spring Harbor Press;
    [Google Scholar]
  18. Moar M. H., Campo M. S., Laird H., Jarrett W. F. H. 1981; Persistence of non-integrated viral DNA in bovine cells transformed in vitro by bovine papillomavirus type 2. Nature, London 293:749–751
    [Google Scholar]
  19. Noyes W. F., Mellors R. C. 1957; Fluorescent antibody detection of the antigens of the Shope papilloma virus in papillomas of the wild and domestic rabbit. Journal of Experimental Medicine 106:555–562
    [Google Scholar]
  20. Orth G., Jeanteur P., Croissant O. 1971; Evidence for and localization of vegetative viral DNA replication by autoradiographic detection of RNA-DNA hybrids in sections of tumors induced by Shope papilloma virus. Proceedings of the National Academy of Sciences, U. S. A 68:1876–1880
    [Google Scholar]
  21. Orth G., Breitburd F., Favre M., Croissant O. 1977; Papillomaviruses: possible role in human cancer. Cold Spring Harbor Conferences on Cell Proliferation 4:1043–1068
    [Google Scholar]
  22. Pfister H., Fink B., Thomas C. 1981; Extrachromosomal bovine papillomavirus type 1 DNA in hamster fibromas and fibrosarcomas. Virology 115:414–418
    [Google Scholar]
  23. Rheinwald J. G., Green H. 1975; Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6:331–344
    [Google Scholar]
  24. Rheinwald J. G., Green H. 1977; Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature, London 265:421–424
    [Google Scholar]
  25. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  26. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  27. Strohman R. C., Moss P. S., Micou-Eastwood J., Spector D., Przybyla A., Paterson B. 1977; Messenger RNA for myosin polypeptides: isolation from single myogenic cell cultures. Cell 10:265–273
    [Google Scholar]
  28. Than R. 1978; The binding site on SV40 DNA for a T antigen-related protein. Cell 13:165–179
    [Google Scholar]
  29. Walter E. L., Walker D. L., Cooper G. A. 1965; Localization of specific antigen in human warts. Archives of Pathology 79:419–423
    [Google Scholar]
  30. Wettstein F. O., Stevens J. G. 1982; Variable-sized free episomes ofShope papilloma virus DNA are present in all non-virus producing neoplasms and integrated episomes are detected in some. Proceedings of the National Academy of Sciences, U. S. A 79:790–794
    [Google Scholar]
  31. Zur Hausen H. 1977; Human papilloma viruses and their possible role in squamous cell carcinomas. Current Topics in Microbiology and Immunology 78:1–30
    [Google Scholar]
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