1887

Abstract

SUMMARY

The synthesis of virus polypeptides in rat XC cells infected with herpes simplex virus type 1 (HSV-1; 13VB4C75) was studied. At the permissive temperature the virus induced the synthesis, in a cascade fashion, of significant amounts of several early polypeptides (ICP 6, 8 and 39) and those late polypeptides that are relatively resistant to inhibition by phosphonoacetic acid in HEp2 cells (ICP 5, 11, 25, 29, 43 and 44). The infectious cycle appeared to become arrested in XC cells at about 7 to 9 h post-infection, because the relative concentrations of early and latest polypeptides labelled thereafter remained constant and the levels of several of the late virus polypeptides were severely reduced (ICP 2, 10, 24 and 26) or not synthesized at all (ICP 32, 34 and 37). When XC cells were infected at a very high m.o.i., only a small amount of virus DNA synthesis could be detected; the synthesis of cellular DNA was not impaired and the infected XC cells continued to replicate for several weeks at least. When XC cells were infected at the non-permissive temperature, only the immediate-early (IE) ICP 4 could be detected while IE ICP 0 and 22 were not observed. Infection of XC cells with HSV-1 (MP) also resulted in the production of early and late viral polypeptides. On the other hand, in XC cells infected with HSV-1 (F) and HSV-1 (HFEM), the synthesis of virus polypeptides could not be detected.

Keyword(s): HSV , protein synthesis and XC cells
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-7-1499
1983-07-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/7/JV0640071499.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-7-1499&mimeType=html&fmt=ahah

References

  1. Becker Y., Shlomai J., Asher Y., Weinberg E., Cohen Y., Olshevsky U., Kotler M. 1974; Interaction of herpes simplex virus type 1 with Rous sarcoma virus-transformed rat cells (XC and R(B77) cell lines). Intervirology 4:325–332
    [Google Scholar]
  2. Campbell W. F., Murray B. K., Biswal N., Benyesh-Melnick M. 1974; Restriction of herpes simplex virus type 1 replication in oncornavirus transformed cells. Journal of the National Cancer Institute 52:757–761
    [Google Scholar]
  3. Conley A., Knipe D. M., Jones P. C., Roizman B. 1981; Molecular genetics of herpes simplex virus. VII. Characterisation of a temperature sensitive mutant produced by in vitro mutagenesis and defective in DNA synthesis and accumulation of y polypeptides. Journal of Virology 37:191–206
    [Google Scholar]
  4. Docherty J. J., Mitchel W. R., Thompson C. J. 1973; Abortive herpes simplex virus replication in Rous sarcoma virus transformed cells (37665). Proceedings of the Society for Experimental Biology and Medicine 144:697–704
    [Google Scholar]
  5. Epstein A., Jacquemont B., Huppert J. 1980; Herpes simplex type 1 infection of nonpermissive rat XC cells. Archives of Virology 63:43–55
    [Google Scholar]
  6. Fenwick M., Morse L. S., Roizman B. 1979; Anatomy of herpes simplex virus DNA. XI. Apparent clustering of functions effecting rapid inhibition of host DNA and protein synthesis. Journal of Virology 29:825–827
    [Google Scholar]
  7. Garfinkle B., McAuslan B. R. 1973; Non-cytopathic, non-productive infection by herpes simplex viruses types 1 and 2. Intervirology 1:362–365
    [Google Scholar]
  8. Garfinkle B., McAuslan B. R. 1974; Regulation of herpes simplex virus-induced thymidine kinase. Biochemical and Biophysical Research Communications 58:822–829
    [Google Scholar]
  9. Halliburton L. W. 1980; Intertypic recombinants of herpes simplex viruses. Journal of General Virology 48:1–23
    [Google Scholar]
  10. Heine J. W., Honess R. W., Cassai E., Roizman B. 1974; Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains. Journal of Virology 14:640–651
    [Google Scholar]
  11. Hoggan M. D., Roizman B. 1959; The isolation and properties of a variant of herpes simplex producing multinucleated giant cells in monolayer cultures in the presence of antibody. American Journal of Tropical Medicine and Hygiene 70:208–219
    [Google Scholar]
  12. Honess R. W., Roizman B. 1973; Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and non-structural herpesvirus polypeptides in the infected cell. Journal of Virology 12:1347–1365
    [Google Scholar]
  13. Honess R. W., Buchan A., Halliburton I. W., Watson D. H. 1980; Recombination and linkage between structural and regulatory genes of herpes simplex virus type 1: study of the functional organisation of the genome. Journal of Virology 34:716–742
    [Google Scholar]
  14. Moorse L. S., Pereira L., Roizman B., Schaffer P. A. 1978; Anatomy of HSV DNA. XI. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 × HSV-2 recombinants. Journal of Virology 26:389–410
    [Google Scholar]
  15. NotaRianni E. L., Preston C. M. 1982; Activation of cellular stress protein genes by herpes simplex virus temperature-sensitive mutants which overproduce immediate early polypeptides. Virology 123:113–122
    [Google Scholar]
  16. Padgett R. A., Moore D. F., Kingsbury D. T. 1978; Herpes simplex virus nucleic acid synthesis following infection of non-permissive XC cells. Journal of General Virology 40:605–614
    [Google Scholar]
  17. Ruyechan W. T., Morse L. S., Knipe D. M., Roizman B. 1979; Molecular genetics of herpes simplex virus. II. Mapping of the major viral glycoproteins and of the genetic loci specifying the social behavior of infected cells. Journal of Virology 29:677–697
    [Google Scholar]
  18. Svoboda J. 1960; Presence of chicken tumor virus in the sarcoma of the adult rat inoculated after birth with Rous sarcoma tissue. Nature, London 186:980–981
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-7-1499
Loading
/content/journal/jgv/10.1099/0022-1317-64-7-1499
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error