1887

Abstract

SUMMARY

Temperature-sensitive () mutants of Newcastle disease virus (NDV) were isolated and studied for interferon (IFN) induction in primary chick embryo (CE) cells. At the non-permissive temperature (41 °C), there was no viral RNA synthesis or IFN induction by u.v.-treated virions except for -3 (RNA), which did synthesize RNA at 41 °C, and whose u.v.-treated virions did induce IFN at this temperature. Another mutant (-4) induced IFN without irradiation, at the permissive temperature (37 °C). The minimum u.v. target size for IFN inducibility was unaffected by the mutation and corresponded to about 5% of the genome required for the expression of infectivity. These results support the hypothesis that the appearance of NDV RNA immediately after infection (primary transcription) plays a key role in IFN induction.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-7-1469
1983-07-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/7/JV0640071469.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-7-1469&mimeType=html&fmt=ahah

References

  1. Atkins G. J., Lancashire C. L. 1976; The induction of interferon by temperature-sensitive mutants of Sindbis virus: its relationship to double-stranded RNA synthesis and cytopathic effect. Journal of General Virology 30:157–165
    [Google Scholar]
  2. Carver D. H., Marcus P. I. 1967; Enhanced interferon production from chick embryo cells aged in vitro. Virology 32:247–257
    [Google Scholar]
  3. Clavell L. A., Bratt M. A. 1971; Relationship between the ribonucleic acid-synthesizing capacity of ultraviolet-irradiated Newcastle disease virus and its ability to induce interferon. Journal of Virology 8:500–508
    [Google Scholar]
  4. Finter N. B. 1969; Dye uptake methods for assessing viral cytopathogenicity and their application to interferon assay. Journal of General Virology 5:419–127
    [Google Scholar]
  5. Kohase M., Vilcek J. 1979; Interferon induction with Newcastle disease virus in FS-4 cells: effect of priming with interferon and of virus inactivating treatments. Japanese Journal of Medical Science and Biology 32:281–293
    [Google Scholar]
  6. Kohase M., Kohno S., Yamazaki S., Shishido A., Kono R. 1982; Potency standardization of human interferon preparations for clinical trials. In The Clinical Potential of Interferons pp 299–309 Edited by Kono R., Vilcek J. Tokyo: University of Tokyo Press;
    [Google Scholar]
  7. Kohno S., Kohase M., Suganuma M. 1968; Inhibition of interferon production by chloroquine diphosphate. Japanese Journal of Medical Science and Biology 21:239–248
    [Google Scholar]
  8. Kohno S., Kohase M., Shimizu Y. 1969; Studies on interferon induction by Newcastle disease virus (NDV). II. Interferon induction by heat- and acid-treated NDV. Archiv für die gesamte Virusforschung 28:188–196
    [Google Scholar]
  9. Kowal K. J., Youngner J. S. 1978; Induction of interferon by temperature sensitive mutants of Newcastle disease virus. Virology 90:90–102
    [Google Scholar]
  10. Lomniczi B. 1973; Studies on interferon production and interferon sensitivity of different strains of Newcastle disease virus. Journal of General Virology 21:305–313
    [Google Scholar]
  11. Lomniczi B., Burke D. C. 1970; Interferon production by temperature sensitive mutants of Semliki Forest virus. Journal of General Virology 8:55–68
    [Google Scholar]
  12. Mcklmm J., Rapp F. 1977; Inability of measles virus temperature-sensitive mutants to induce interferon. Virology 76:409–415
    [Google Scholar]
  13. Marcus P. I., Fuller F. J. 1979; Interferon induction by viruses. II. Sindbis virus: interferon induction requires one-quarter of the genome – genes G and A. Journal of General Virology 44:169–177
    [Google Scholar]
  14. Marcus P. I., Sekellick M. J. 1980; Interferon induction by viruses. III. Vesicular stomatitis virus: interferon-inducing particle activity requires partial transcription of gene N. Journal of General Virology 47:89–96
    [Google Scholar]
  15. Meager A., Burke D. C. 1972; Production of interferon by ultraviolet irradiated Newcastle disease virus. Nature, London 235:280–282
    [Google Scholar]
  16. Sekellick M. J., Marcus P. I. 1979; Persistent infection. II. Interferon-inducing temperature-sensitive mutants as mediators of cell sparing: possible role in persistent infection by vesicular stomatitis virus. Virology 95:36–47
    [Google Scholar]
  17. Sheaff E. T., Meager A., Burke D. C. 1972; Factors involved in the production of interferon by inactivated Newcastle disease virus. Journal of General Virology 17:163–175
    [Google Scholar]
  18. Stewart W. E. II, Gosser L. B., Lockart R. Z. 1971; Priming: a non-antiviral function of interferon. Journal of Virology 7:792–801
    [Google Scholar]
  19. Youngner J. S., Scott A. W., Hallum J. V., Stinebring W. R. 1966; Interferon production by inactivated Newcastle disease virus in cell cultures and in mice. Journal of Bacteriology 92:862–868
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-7-1469
Loading
/content/journal/jgv/10.1099/0022-1317-64-7-1469
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error