1887

Abstract

SUMMARY

Ibaraki virus core particles were purified from infected BHK-21 cells. The core particles displayed RNA transcriptase activity while the virion did not. The RNA transcriptase activity was stimulated about 15-fold by addition of -adenosyl--methionine. The reaction product was single-stranded RNA which could be hybridized with heat-denatured Ibaraki virus RNA. The hybrids possessed the same electrophoretic mobility as Ibaraki virus double-stranded RNA.

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/content/journal/jgv/10.1099/0022-1317-64-6-1397
1983-06-01
2022-01-23
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References

  1. Furuichi Y. 1974; ‘Methylation-coupled’ transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA. Nucleic Acids Research 1:809–822
    [Google Scholar]
  2. Huismans H., Verwoerd D. W. 1973; Control of transcription during the expression of the bluetongue virus genome. Virology 52:81–88
    [Google Scholar]
  3. Lewandowski L. J., Kalmakoff J., Tanada Y. 1969; Characterization of a ribonucleic acid polymerase activity associated with purified cytoplasmic polyhedrosis virus of the silkworm Bombyx mori. Journal of Virology 4:857–865
    [Google Scholar]
  4. Loening U. E. 1969; The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. The effects of changes in conformation. Biochemical Journal 113:131–138
    [Google Scholar]
  5. Martin S. A., Zweerink H. J. 1972; Isolation and characterization of two types of bluetongue virus particles. Virology 50:495–506
    [Google Scholar]
  6. Omori T., Inaba Y., Morimoto T., Tanaka Y., Ishitani R., Kurogl H., Munakata K., Matsuda K., Matumoto M. 1969; Ibaraki virus, an agent of epizootic disease of cattle resembling bluetongue. I. Epidemiologic, clinical and pathologic observations and experimental transmission to calves. Japanese Journal of Microbiology 13:139–157
    [Google Scholar]
  7. Suzuki Y., Saito Y., Nakagawa S. 1977; Double-stranded RNA of Ibaraki virus. Virology 76:670–674
    [Google Scholar]
  8. Suzuki Y., Nakagawa S., Namiki M., Saito Y. 1978; RNA and protein of Ibaraki virus. Kitasato Archives ofExperimental Medicine 51:61–71
    [Google Scholar]
  9. Van Dijk A. A., Huismans H. 1980; The in vitro activation and further characterization of the bluetongue virus-associated transcriptase. Virology 104:347–356
    [Google Scholar]
  10. Verwoerd D. W., Huismans H. 1972; Studies on the in vitro and the in vivo transcription of the bluetongue virus genome. Onderstepoort Journal of Veterinary Research 39:185–192
    [Google Scholar]
  11. Watanabe Y., Graham A. F. 1967; Structural units of reovirus ribonucleic acid and their possible functional significance. Journal of Virology 1:665–677
    [Google Scholar]
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