1887

Abstract

Summary

MG63 cells induced by Sendai virus produce human interferon (IFN)-β with a molecular weight of 22000. In the presence of tunicamycin, they produced non-glycosylated, biologically active, IFN-β with a molecular weight of 17000. It had slightly lower heat stability (at 56 °C) than normal glycosylated IFN, but the sensitivity to neutralization by antisera and the cross-species activities on murine, bovine and hamster cells were unaltered, indicating dispensability of the carbohydrate moiety for these parameters. However, the IFN yields were markedly reduced by the glycosylation inhibitor, suggesting an important role of glycosylation in production by cells of IFN-β.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-6-1391
1983-06-01
2019-10-19
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-6-1391
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error