@article{mbs:/content/journal/jgv/10.1099/0022-1317-64-6-1369, author = "Tenser, Richard B. and Ressel, Stephen J. and Fralish, F. Anne and Jones, John C.", title = "The Role of Pseudorabies Virus Thymidine Kinase Expression in Trigeminal Ganglion Infection", journal= "Journal of General Virology", year = "1983", volume = "64", number = "6", pages = "1369-1373", doi = "https://doi.org/10.1099/0022-1317-64-6-1369", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-6-1369", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "PRV infection", keywords = "trigeminal ganglion infection", keywords = "PRV thymidine kinase", abstract = "SUMMARY The role of pseudorabies virus (PRV) thymidine kinase (TK) expression in the pathogenesis of PRV infection of mice was studied with TK-negative (TK−) mutants. Thymidine phosphorylation and arabinosylthymine inhibition of PRV replication and efficiency of plating were used to characterize TK+ and TK− PRV. In addition, a plaque autoradiography procedure was utilized to determine the TK phenotype of individual plaques. TK+ and TK− PRV replicated well in ocular tissues, while TK+ but not TK− did so in ganglion tissue. Mortality was absent after TK− PRV inoculation and widespread after inoculation of similar amounts of TK+ PRV. Latent infection in mice was not detected with either TK+ or TK− PRV. This study indicated the probable importance of PRV TK expression in acute trigeminal ganglion infection.", }