1887

Abstract

SUMMARY

The role of pseudorabies virus (PRV) thymidine kinase (TK) expression in the pathogenesis of PRV infection of mice was studied with TK-negative (TK) mutants. Thymidine phosphorylation and arabinosylthymine inhibition of PRV replication and efficiency of plating were used to characterize TK and TK PRV. In addition, a plaque autoradiography procedure was utilized to determine the TK phenotype of individual plaques. TK and TK PRV replicated well in ocular tissues, while TK but not TK did so in ganglion tissue. Mortality was absent after TK PRV inoculation and widespread after inoculation of similar amounts of TK PRV. Latent infection in mice was not detected with either TK or TK PRV. This study indicated the probable importance of PRV TK expression in acute trigeminal ganglion infection.

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/content/journal/jgv/10.1099/0022-1317-64-6-1369
1983-06-01
2024-12-13
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References

  1. Field H. J., Hill T. J. 1974; The pathogenesis of pseudorabies in mice following peripheral inoculation. Journal of General Virology 23:145–157
    [Google Scholar]
  2. Field H. J., Wildy P. 1978; The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice. Journal of Hygiene 81:267–277
    [Google Scholar]
  3. Galloway D. A., Fenoglio C. M., McDougall J. K. 1982; Limited transcription of the herpes simplex virus genome when latent in human sensory ganglia. Journal of Virology 41:686–691
    [Google Scholar]
  4. Gutekunst D. E., Pirtle E. C., Miller L. D., Stewart W. C. 1980; Isolation of pseudorabies virus from trigeminal ganglia of a latently infected sow. American Journal of Veterinary Research 41:1315–1316
    [Google Scholar]
  5. Hamada C., Kamiya T., Kaplan A. S. 1966; Serological analysis of some enzymes present in pseudorabies virus-infected and noninfected cells. Virology 28:271–281
    [Google Scholar]
  6. Klein R. J., De Stefano E., Brady E., Friedman-Kien A. E. 1980; Experimental skin infection with an acyclovir resistant herpes simplex virus mutant: response to antiviral treatment and protection against reinfection. Archives of Virology 65:237–246
    [Google Scholar]
  7. McCracken R. M., McFerran J. B., Dow C. 1973; The neural spread of pseudorabies virus in calves. Journal of General Virology 20:17–28
    [Google Scholar]
  8. Sabó A., Rajcáni J. 1976; Latent pseudorabies virus infection in pigs. Acta virologica 20:208–214
    [Google Scholar]
  9. Tenser R. B., Dunstan M. E. 1979; Herpes simplex virus thymidine kinase expression in infection of the trigeminal ganglion. Virology 99:417–422
    [Google Scholar]
  10. Tenser R. B., Ressel S., Dunstan M. E. 1981; Herpes simplex virus thymidine kinase expression in trigeminal ganglion infection: correlation of enzyme activity with ganglion virus titer and evidence of in vivo complementation. Virology 112:328–341
    [Google Scholar]
  11. Tenser R. B., Dawson M., Ressel S. J., Dunstan M. E. 1982; Detection of herpes simplex virus mRNA in latently infected trigeminal ganglion neurons by in situ hybridization. Annals of Neurology 11:285–291
    [Google Scholar]
  12. Tenser R. B., Jones J. C., Ressel S. J., Fralish F. A. 1983; Thymidine plaque autoradiography of thymidine kinase-positive and thymidine kinase-negative herpesviruses. Journal of Clinical Microbiology 17:122–127
    [Google Scholar]
  13. Wittmann G., Jakubik J., Ahl R. 1980; Multiplication and distribution of Aujeszky’s disease (pseudorabies) virus in vaccinated and non-vaccinated pigs after intranasal infection. Archives of Virology 66:227–240
    [Google Scholar]
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