1887

Abstract

SUMMARY

The effects of amber mutations in the 24 known essential genes of phage T1 on phage-directed protein synthesis were examined by SDS-polyacrylamide gel electrophoresis of radiolabelled polypeptides from infected non-permissive cells. Mutations in nine genes (genes 1, 2, 3, 3.5, 5, 7–8, 10, 13.3 and 15) each resulted in the failure to synthesize a single polypeptide. Since the synthesis of each of these polypeptides was at least partially restored in permissive infections and wholly restored in revertant infections we conclude that the affected polypeptide is the primary gene product. Mutations in genes 13.7, 16 and 17, which are required for head formation, are all defective in the synthesis of a 68000 mol. wt. non-structural polypeptide. Mutants in the head genes 13 and 14 fail to cleave P7p to P7, the major structural component of proheads. Gene 14 mutants also fail to make a 45000 mol. wt. non-structural polypeptide which appears to be involved with the gene 13 product in the P7p cleavage reaction. The protein products of the DNA genes 1, 2 and 3.5 are, as expected, synthesized predominantly early in infection whereas those of the remainder, which determine head and tail formation, are made in greater amounts late in infection. Gene 3, a tail gene which has been mapped within the ‘early’ DNA gene cluster, codes for a 75000 mol. wt. polypeptide synthesized predominantly late in infection. This observation suggests that the early genes 1, 2, 3.5 and 4 do not form an operon under the control of a single early promoter.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-6-1355
1983-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/6/JV0640061355.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-6-1355&mimeType=html&fmt=ahah

References

  1. Anderson E. H. 1946; Growth requirements of virus-resistant mutants of Escherichia coli strain B. Proceedings ofthe National Academy of Sciences U. S. A 32:120–128
    [Google Scholar]
  2. Borchert L. D., Drexler H. 1980; T1 genes which affect transduction. Journal of Virology 33:1122–1128
    [Google Scholar]
  3. Bourque L. W., Christensen J. R. 1980; The synthesis of coliphage T1 DNA: requirement for host DNAgenesinvolved in elongation. Virology 102:310–316
    [Google Scholar]
  4. Drexler H., Christensen J. R. 1961; Genetic crosses between restricted and non-restricted phage T1 inlysogenic and non-lysogenic hosts. Virology 13:31–39
    [Google Scholar]
  5. Figurski D., Christensen J. R. 1974; Functional characterisation of the genes of bacteriophage Tl. Virology 59:397–407
    [Google Scholar]
  6. Martin D. T. M., Adair C. A., Ritchie D. A. 1976; Polypeptides specified by bacteriophage Tl. Journal ofGeneral Virology 33:309–319
    [Google Scholar]
  7. Michalke W. 1967; ErhohteRekombinationshaufigeit an den Enden des T1-chromosoms. Molecular and GeneralGenetics 99:19–33
    [Google Scholar]
  8. Ramsay N. 1981; DNA morphology and phage particle assembly in bacteriophage T1. Ph.D. thesis University of Liverpool;
    [Google Scholar]
  9. Ramsay N., Ritchie D. A. 1982; Restriction endonuclease analysis of variants of phage Tl: correlation of thephysical and genetic maps. Virology 121:420–425
    [Google Scholar]
  10. Ritchie D. A., Joicey D. H. 1980; Identification of some steps in the replication of bacteriophage Tl DNA. Virology 103:191–198
    [Google Scholar]
  11. Ritchie D. A., Christensen J. R., Pugh J. C., Bourque L. W. 1980; Genes of coliphage T1 whose productspromote general recombination. Virology 105:371–378
    [Google Scholar]
  12. Toni M., Conti G., Schito G. C. 1976; Viral protein synthesis during replication of bacteriophage T1. Biochemical and Biophysical Research Communications 68:545–552
    [Google Scholar]
  13. Trautner T. A. 1960; Genetische und physiologischeBeziehungenZwischen den Bakteriophagen T1 und D20. Zeitschrift für Vererbungslehre 91:317–324
    [Google Scholar]
  14. Wagner E. F., Ponta H., Schweiger M. 1977; Development of E. coli virus Tl: the pattern of gene expression. Molecular and General Genetics 150:21–28
    [Google Scholar]
  15. Walling L., Christensen J. R. 1981; The synthesis of coliphage T1 DNA: studies on the roles of T1 genes 1, 2and 4. Virology 114:309–318
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-6-1355
Loading
/content/journal/jgv/10.1099/0022-1317-64-6-1355
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error