1887

Abstract

SUMMARY

We report the ultrastructural features associated with wild-type and temperature-sensitive () mutant infection at non-permissive temperature for each of 18 herpes simplex virus type 1 (HSV-1) (strain 17) mutants. The mutants were classified by their ability to induce nucleocapsid-related structures:class I failed to induce any, class II induced empty and partially filled structures while class III induced all of the identified structures including those containing an electron-dense core. The time when expression of the lesion blocked virus replication was estimated for most mutants; this allows both mutant gene expression and the resulting ultrastructural features to be correlated with the sequence of virus replicative events. Three mutant-triggered features not previously described in HSV-1-infected cells were also recognized: a modification of rough endoplasmic reticulum, intranuclear accumulation of enveloped virus particles and cytoplasmic accumulation of novel doughnut-shaped particles having a concentric double ring appearance in thin section.

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1983-06-01
2022-01-19
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References

  1. Atkinson M. A., Barr S., Timbury M. C. 1978; The fine structure of cells infected with temperature-sensitive mutants of herpes simplex virus type 2. Journal of General Virology 40:103–119
    [Google Scholar]
  2. Brown S. M., Ritchie D. A., Subak-Sharpe J. H. 1973; Genetic studies with herpes simplex virus type 1. The isolation of temperature-sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map. Journal of General Virology 18:329–346
    [Google Scholar]
  3. Cabral G. A., Schaffer P. A. 1976; Electron microscope studies of temperature-sensitive mutants of herpes simplex virus type 2. Journal of Virology 18:727–737
    [Google Scholar]
  4. Cabral G. A., Courtney R. J., Schaffer P. A., Marciano-Cabral F. 1980; Ultrastructural characterisation of an early nonstructural polypeptide of herpes simplex virus type 1. Journal of Virology 33:1192–1198
    [Google Scholar]
  5. Crombie I. K. 1975; Genetic and biochemical studies with herpes simplex virus type 1. Ph.D. thesis University of Glasgow;
    [Google Scholar]
  6. Darlington R. W., Moss L. H. 1968; Herpesvirus envelopment. Journal of Virology 2:48–55
    [Google Scholar]
  7. Friedmann A., Coward J. E., Rosenkranz H. S., Morgan C. 1975; Electron microscopy studies on assembly of herpes simplex virus upon removal of hydroxyurea block. Journal of General Virology 26:171–181
    [Google Scholar]
  8. Gerdes J. C., Marsden H. S., Cook M. L., Stevens J. G. 1979; Acute infection of differentiated neuroblastoma cells by latency-positive and latency-negative herpes simplex virus ts mutants. Virology 94:430–441
    [Google Scholar]
  9. Halliburton I. W., Timbury M. C. 1976; Temperature-sensitive mutants of herpes simplex virus type 2: description of three new complementation groups and studies on the inhibition of host cell DNA synthesis. Journal of General Virology 30:207–221
    [Google Scholar]
  10. Heine U., Ablashi D. V., Armstrong G. R. 1971; Morphological studies on herpesvirus saimiri in subhuman and human cell cultures. Cancer Research 31:1019–1029
    [Google Scholar]
  11. McCombs R. M., Brunschwig J. P., Mirkovic R., Benyesh-Melnick M. 1971; Electron microscopic characterisation of a herpeslike virus isolated from Tree Shrews. Virology 45:816–820
    [Google Scholar]
  12. Macdonald D. 1980; Herpes simplex virus-induced early proteins. Ph.D. thesis University of Glasgow;
    [Google Scholar]
  13. Marsden H. S., Crombie I. K., Subak-Sharpe J. H. 1976; Control of protein synthesis in herpesvirus-infected cells: analysis of the polypeptides induced by wild type and sixteen temperature-sensitive mutants of HSV strain 17. Journal of General Virology 31:347–372
    [Google Scholar]
  14. Miyamoto K., Morgan C., Hsu K. C., Hampar B. 1971; Differentiation by immunoferritin of herpes simplex virion antigens with the use of rabbit 7S and 19S antibodies from early (7 day) and late (7 week) immune sera. Journal of the National Cancer Institute 46:629–646
    [Google Scholar]
  15. Morgan C., Rose H. M., Mednis B. 1968; Electron microscopy of herpes simplex virus. I. Entry. Journal of Virology 2:507–516
    [Google Scholar]
  16. Nazerian K., Lee L. F., Witter R. L., Burmester B. R. 1971; Ultrastructural studies of a herpesvirus of turkeys antigenically related to Marek’s disease virus. Virology 43:442–452
    [Google Scholar]
  17. Nii S. 1971a; Electron microscope observations on FL cells infected with herpes simplex virus. (I) Viral forms. Biken Journal 14:177–190
    [Google Scholar]
  18. Nii S. 1971b; Electron microscope observations on FL cells infected with herpes simplex virus. (II) Envelopment. Biken Journal 14:325–348
    [Google Scholar]
  19. Nii S. 1976; Double arc structures in nuclei infected with human cytomegalovirus. Biken Journal 19:159–163
    [Google Scholar]
  20. Nii S., Morgan C., Rose H. M. 1968a; Electron microscopy of herpes simplex virus. (II) Sequence of development. Journal of Virology 2:517–536
    [Google Scholar]
  21. Nii S., Rosenkranz H. S., Morgan C., Rose H. M. 1968b; Electron microscopy of herpes simplex virus. (III) Effect of hydroxyurea. Journal of Virology 2:1163–1172
    [Google Scholar]
  22. Nii S., Morgan C., Rose H. M., Hsu K. C. 1968c; Electron microscopy of herpes simplex virus. (IV) Studies with ferritin-conjugated antibodies. Journal of Virology 2:1172–1184
    [Google Scholar]
  23. Okada K., Fujimoto Y., Mikami T. 1974; Electron microscopic autoradiography in the development of herpes virus of turkey. Archiv für die gesamte Virusforschung 46:111–126
    [Google Scholar]
  24. Perdue M. L., Cohen J. C., Randall C. C., O’Callaghan D. J. 1976; Biochemical studies of the maturation of herpesvirus nucleocapsid species. Virology 74:194–208
    [Google Scholar]
  25. Preston C. M. 1979; Abnormal properties of an immediate early polypeptide in cells infected with the herpes simplex virus type 1 mutant ts K. Journal of Virology 32:357–369
    [Google Scholar]
  26. Preston V. G. 1981; Fine-structure mapping of herpes simplex virus type 1 temperature sensitive mutations within the short repeat region of the genome. Journal of Virology 39:150–161
    [Google Scholar]
  27. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–228
    [Google Scholar]
  28. Roizman B., Spear P. G. 1971; Herpesvirus antigens and cell membranes detected by centrifugation of membrane-antibody complexes. Science 171:298–300
    [Google Scholar]
  29. Schaffer P. A., Brunschwig J. P., McCombs R. M., Benyesh-Melnick M. 1974; Electron microscopic studies of temperature-sensitive mutants of herpes simplex virus type 1. Virology 62:444–457
    [Google Scholar]
  30. Schwartz J., Roizman B. 1969; Concerning the egress of herpes simplex virus from infected cells: electron and light microscope observations. Virology 38:42–49
    [Google Scholar]
  31. Stow N. D. 1978 Physical mapping of herpes simplex virus type 1 mutations Ph.D. thesis University of Glasgow;
    [Google Scholar]
  32. Stow N. D., Subak-Sharpe J. H., Wilkie N. M. 1978; Physical mapping of herpes simplex virus type 1 mutations by marker rescue. Journal of Virology 28:182–192
    [Google Scholar]
  33. Sydiskis R. J., Roizman B. 1966; Polysomes and protein synthesis in cells infected with a DNA virus. Science 153:76–78
    [Google Scholar]
  34. Sydiskis R. J., Roizman B. 1967; The disaggregation of host polyribosomes in productive and abortive infection with herpes simplex virus. Virology 32:678–686
    [Google Scholar]
  35. Sydiskis R. J., Roizman B. 1968; The sedimentation profiles of cytoplasmic polyribosomes in mammalian cells productively and abortively infected with herpes simplex virus. Virology 34:562–565
    [Google Scholar]
  36. Watson R. J., Clements J. B. 1978; Characterisation of transcription-deficient temperature-sensitive mutants of herpes simplex virus type 1. Virology 91:364–379
    [Google Scholar]
  37. Watson R. J., Clements J. B. 1980; A herpes simplex virus type 1 function continuously required for early and late virus RNA synthesis. Nature, London 285:329–330
    [Google Scholar]
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