1887

Abstract

SUMMARY

Low doses of u.v. radiation rapidly inactivate poliovirus, and the virus is progressively converted into dense particles (DPs) of buoyant density 1.44 g/ml in CsCl. The DPs are structurally and antigenically related to standard virus (N-antigen), i.e. they are indistinguishable from virus in their RNA and protein content and in their sedimentation properties. Furthermore, there is no difference in reactivity of the structural proteins of virus and DPs with the monofunctional reagent [H]-succinimidyl propionate (H-NSP). However, DPs differ from virus in that their capsids are permeable to several ions, and they can be degraded by RNase and protease. Increasing the radiation dose causes a successive transformation of DPs into 105S slow-sedimenting particles (SSPs). The SSPs are antigenically related to 76S artificial empty capsids (AECs) or H-antigen, but they differ physically and structurally from them. The SSPs have a higher S value than AECs and contain all the capsid proteins, including VP4, and the RNA, both of these macromolecules being absent from AECs. It is concluded, therefore, that transformation from N- to H-antigenicity by u.v. radiation does not require release of RNA and VP4. Conversion of virus particles to SSPs correlates with altered reactivity of VP2 and to a lesser extent VP1 and VP3, with the monofunctional reagent H-NSP.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-6-1263
1983-06-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/6/JV0640061263.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-6-1263&mimeType=html&fmt=ahah

References

  1. Beneke T. W., Habermehl K.-O., Diefenthal E., Buchholz M. 1977; Iodination of poliovirus capsid proteins. Journal of General Virology 34:387–390
    [Google Scholar]
  2. Boublik M., Drzeniek R. 1976; Demonstration of a core in poliovirus particles by electron microscopy. Journal of General Virology 31:447–449
    [Google Scholar]
  3. Boublik M., Drzeniek R. 1977; Structural subunits of poliovirus particles by electron microscopy. Journal of General Virology 37:127–134
    [Google Scholar]
  4. Breindl M. 1971; The structure of heated poliovirus particles. Journal of General Virology 11:147–156
    [Google Scholar]
  5. Chamberlain J. P. 1979; Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Analytical Biochemistry 98:132–135
    [Google Scholar]
  6. Cova-Baczko L., Aymard M. 1982; Echovirus 11 dense particles: isolation and preliminary characterization. Journal of General Virology 60:159–163
    [Google Scholar]
  7. Jacrot B., Chauvin C., Witz I. 1977; Comparative neutron small-angle scattering study of small spherical RNA viruses. Nature, London 266:417–421
    [Google Scholar]
  8. Katagiri S., Hinuma Y., Ishida N. 1967; Biophysical properties of poliovirus particles irradiated with ultraviolet light. Virology 32:337–343
    [Google Scholar]
  9. Katagiri S., Aikawa S., Hinuma Y. 1971; Stepwise degradation of poliovirus capsid by alkaline treatment. Journal of General Virology 13:101–109
    [Google Scholar]
  10. Kitamura N., Semler B. L., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E. A., Hanecak R., Lee J. J., van der werf S., Anderson C. W., Wimmer E. 1981; Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature, London 291:547–553
    [Google Scholar]
  11. Le Bouvier G. L. 1959; The D-C change in poliovirus particles. British Journal of Experimental Pathology 40:605–620
    [Google Scholar]
  12. Lonberg-Holm K., Butterworth B. E. 1976; Investigation of the structure of polio- and human rhinovirus through the use of selective chemical reactivity. Virology 71:207–216
    [Google Scholar]
  13. Mapoles J. E., Anderegg J. W., Rueckert R. R. 1978; Properties of poliovirus propagated in medium containing cesium chloride: implications for picornaviral structure. Virology 90:103–111
    [Google Scholar]
  14. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behavior of enzymes: application to protein mixtures. Journal of Biological Chemistry 236:1372–1379
    [Google Scholar]
  15. Miller R. L., Plagemann P. G. W. 1974; Effect of ultraviolet light on mengovirus: formation of uracil dimers, instability and degradation of capsid, and covalent linkage of protein to viral RNA. Virology 13:729–739
    [Google Scholar]
  16. Ouceiterlony O. 1958; Diffusion in gel. Methods for immunological analysis. Progress in Allergy 5:1–78
    [Google Scholar]
  17. Perez-Bercoff R., Gander M., Preisig E. 1978; The genomic RNA of mengovirus. II. Comparative analysis of the standard and denser virions. Virology 85:378–386
    [Google Scholar]
  18. Putnak J. R., Phillips B. A. 1982; Poliovirus empty capsid morphogenesis: evidence for conformational differences between self- and extract-assembled empty capsids. Journal of Virology 41:792–800
    [Google Scholar]
  19. Roizman B., Mayer M. M., Roane P. R. Jr 1959; Immunochemical studies of poliovirus. Journal of Immunology 82:19–25
    [Google Scholar]
  20. Rowlands D. J., Shirley M. W., Sangar D. V., Brown F. 1975; A high-density component in several vertebrate enteroviruses. Journal of General Virology 29:223–234
    [Google Scholar]
  21. Rueckert R. R. 1976; On the structure and morphogenesis of picomaviruses. In Comprehensive Virology vol 6: pp 131–213 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
    [Google Scholar]
  22. Sangar D. V., Rowlands D. J., Smale C. J., Brown F. 1973; Reaction of glutaraldehyde with foot-and-mouth disease virus. Journal of General Virology 21:399–406
    [Google Scholar]
  23. Schaffer F. L., Schwerdt C. E. 1959; Purification and properties of poliovirus. Advances in Virus Research 6:159–204
    [Google Scholar]
  24. Stöffler G., Hasenbank R., Lütgehaus M., Maschler R., Morrison C. A., Zeichhardt H., Garrett R. A. 1973; The accessibility of the Escherichia coli 30 S ribosoma) subunit to antibody binding. Molecular and General Genetics 127:89–110
    [Google Scholar]
  25. Svedberg T., Pedersen K. O. 1940; Die Ultrazentrifuge. In Handbuch der Kolloidwissenschaft vol 7: pp 26–35 Edited by Ostwald W. Dresden & Leipzig: Steinkopf;
    [Google Scholar]
  26. Vanden Berghe D., Boeye A. 1973; Stepwise degradation of poliovirus and top component by concentrated urea. Archiv fur die gesamte Virus for schung 41:216–228
    [Google Scholar]
  27. Wetz K., Habermehl K.-O. 1979; Topographical studies on poliovirus capsid proteins by chemical modification and cross-linking with bifunctional reagents. Journal of General Virology 44:525–534
    [Google Scholar]
  28. Wetz K., Habermehl K.-O. 1982; Specific cross-linking of capsid proteins to virus RNA by ultraviolet irradiation of poliovirus. Journal of General Virology 59:397–401
    [Google Scholar]
  29. Wiegers K. J., Yamaguchi-Koll U., Drzeniek R. 1977; Differences in the physical properties of dense and standard poliovirus particles. Journal of General Virology 34:465–473
    [Google Scholar]
  30. Wouters M., Miller A. O. A., Fenwick M. L. 1973; Distortion of poliovirus particles by fixation with formaldehyde. Journal of General Virology 18:211–214
    [Google Scholar]
  31. Yamaguchi-Koll U., Wiegers K. J., Drzeniek R. 1975; Isolation and characterization of ‘dense particles’ from poliovirus-infected HeLa cells. Journal of General Virology 26:307–319
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-6-1263
Loading
/content/journal/jgv/10.1099/0022-1317-64-6-1263
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error