1887

Abstract

SUMMARY

Neutralizing monoclonal antibodies raised against intact foot-and-mouth disease virus type O1 reacted with intact virus and trypsin-treated virus particles. Some of the monoclonal antibodies showed a slight but definite reaction with the 12S subunit, but none of them reacted with the isolated capsid protein VP1 or any of the other viral proteins. These results confirm the difference between the neutralizing antigenic determinants exposed on intact virus particle and on isolated capsid protein VP1. They suggest further that the neutralizing antigenic determinant and the cell attachment site are not identical.

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1983-05-01
2024-04-25
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References

  1. Bachrach H. L., Moore D. M., McKercher P. D., Polatnick J. 1975; Immune and antibody responses to an isolated capsid protein of FMDV. Journal of Immunology 115:1636–1641
    [Google Scholar]
  2. Bittle J. L., Houghton R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J., Brown F. 1982; Protection against FMDV by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature, London 298:30–34
    [Google Scholar]
  3. Boothroyd J. C., Highfield P. E., Cross G. A. M., Rowlands D. J., Lowe P. A., Brown F., Harris T. J. R. 1981; Molecular cloning of foot-and-mouth disease virus genome and nucleotide sequences in the structural protein genes. Nature, London 290:800
    [Google Scholar]
  4. Cartwright B., Chapman W. G., Brown F. 1980; Serological and immunological relationships between the 140S and 12S particles of FMDV. Journal of General Virology 50:369–375
    [Google Scholar]
  5. Cartwright B., Morrell D. J., Brown F. 1982; Nature of the antibody response to the foot-and-mouth disease virus particle, its 12S protein subunit and the isolated immunizing polypeptide VP1. Journal of General Virology 63:375–381
    [Google Scholar]
  6. Cooper P. D., Agol V. I., Bachrach H. L., Brown F., Ghendon Y., Gibbs A. J., Gillespie J. H., Lonberg-Holm K., Mandel B., Melnick J. L., Mohanty S. B., Povey R. C., Rueckert R. R., Schaffer F. L., Tyrrell D. A. J. 1978; Picornaviridae: second report. Intervirology 10:165–180
    [Google Scholar]
  7. Fazekas S., Scheidegger D. 1980; Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 35:1–21
    [Google Scholar]
  8. Fraker P. J., Speck J. C. 1978; Protein and cell membrane iodinations with a sparingly soluble chloramide, 1, 3, 4, 6 tetrachloro-3α, 6α-diphenyl glycouril. Biochemical and Biophysical Research Communications 80:849–857
    [Google Scholar]
  9. Hardy M. M., Moore D. M. 1981; Neutralization of foot-and-mouth disease virus. I. Sensitization of the 140S virion by antibody also reactive with the 12S protein subunit. Journal of General Virology 55:415–427
    [Google Scholar]
  10. Icenogle J., Gilbert S. F., Griever J., Anderegg I., Rueckert R. 1981; A neutralizing monoclonal antibody against poliovirus and its reaction with related antigens. Virology 115:211–215
    [Google Scholar]
  11. Kearney F. J., Radbruch A., Liesegan B., Rajewsky K. 1979; A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. Journal of Immunology 123:1548–1550
    [Google Scholar]
  12. Kleid D. G., Yansura D., Small B., Dowbenko D., Moore D. M., Grubman M. J., Morgan D. O., Robertson B. H., Bachrach H. L. 1981; Cloned viral protein vaccine for foot-and-mouth disease. Responses in cattle and swine. Science 214:1125–1129
    [Google Scholar]
  13. Küpper H., Keller W., Kurz C., Forss S., Schaller H., Franze R., Strohmaler K., Marquardt O., Zaslavsky V. G., Hofschneider P. H. 1981; Cloning of cDNA of major antigen of FMDV and expression in E. coli. Nature, London 289:555–559
    [Google Scholar]
  14. Laporte L. M., Grosclaude J., Wantyghem J., Bernard S., Rouze P. 1973; Neutralisation en culture cellulaire du pouvoir infectieux du virus de la fièvre aphteuse par des serums provenant de pores immunisés à l’aide d’une proteine virale purifiée. Compte rendu hebdomadaire des seances de l’Academie des Sciences D276:3399–3402
    [Google Scholar]
  15. Meloen R. H. 1981; Foot-and-mouth disease virus antigens and the antibodies they induce. Thesis, Rijksuniversiteit, Utrecht
    [Google Scholar]
  16. Meloen R. H., Briaire J. 1980; A study of the cross-reacting antigens on the intact foot-and-mouth disease virus and its 12S subunits with antisera against the structural proteins. Journal of General Virology 51:107–116
    [Google Scholar]
  17. Meloen R. H., Rowlands D. J., Brown F. 1979; Comparison of the antibodies elicited by the individual structural proteins of foot-and-mouth disease and polio viruses. Journal of General Virology 45:761–763
    [Google Scholar]
  18. Minor P. D., Kew O., Schild G. C. 1982; Poliomyelitis-epidemiology, molecular biology and immunology. Nature, London 299:109–110
    [Google Scholar]
  19. Rowlands D. J., Sangar D. V., Brown F. 1975; A comparative chemical and serological study of the full and empty particles of FMDV. Journal of General Virology 13:85–93
    [Google Scholar]
  20. Rueckert R. R. 1976; On the structure and morphogenesis of picomaviruses. In Comprehensive Virology vol 6: pp 131–213 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  21. Schneiderman S., Farber J. L., Baserga R. 1979; A simple method for decreasing the toxicity of polyethylene glycol in mammalian cell hybridization. Somatic Cell Genetics 5:263–269
    [Google Scholar]
  22. Strohmaier K., Franze R., Adam K. H. 1982; Location and characterization of the antigenic portion of FMDV immunizing protein. Journal of General Virology 59:295–306
    [Google Scholar]
  23. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U. S. A 76:4350–4354
    [Google Scholar]
  24. Wild T. F., Burroughs J. N., Brown F. 1969; Surface structure of foot-and-mouth disease virus. Journal of General Virology 4:313–320
    [Google Scholar]
  25. Wiley D. C., Wilson I. A., Skehel I. J. 1981; Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation. Nature, London 289:373–378
    [Google Scholar]
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