Expression of Varicella-Zoster Virus-related Antigens in Biochemically Transformed Cells Free

Abstract

SUMMARY

L(O)cl 3 and L(K)cl 1 cells, biochemically transformed by varicella-zoster virus (VZV), were labelled with -[S]methionine and [P]orthophosphate. Cell extracts were immunoprecipitated with anti-VZV monkey serum and analysed by SDS-polyacrylamide gel electrophoresis. Four polypeptides of apparent mol. wt. 135000 (135K), 48K, 44K and 35K were detected in the -[S]methionine-labelled extracts, and, of these, the 35K band showed marked intensity. However, this band was not detected in extracts from cells infected with a VZV strain (Kanno strain). Also, the 35K polypeptide showed very low intensity when immunoprecipitated from extracts of transformed cells grown in non-selective (NS) medium, i.e. cells that had a very low thymidine kinase () activity. In the case of [P]orthophosphate-labelled cells, polypeptides of apparent mol. wt. 180K, 81K, 48K, 44K and 37K were obtained. In both instances, the 44K polypeptide was not immunoprecipitated from L(K)cl 1 cell extracts. From our data it is postulated that the expression of the 35K polypeptide is correlated with the VZV-specific activity of the cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-5-1181
1983-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/5/JV0640051181.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-5-1181&mimeType=html&fmt=ahah

References

  1. Chadha K. C., Munyon W. H. 1975; Presence of herpes simplex virus-related antigens in transformed L cells. Journal of Virology 15:1475–1486
    [Google Scholar]
  2. Chadha K., Munyon W., Zeigel R. F. 1977; Expression of type-specific virion structural antigen(s) in L cells transformed by herpes simplex virus types 1 and 2. Virology 76:433–436
    [Google Scholar]
  3. Cheng Y. C., Tsou T. T., Hackstadt T., Mallavia L. P. 1979; Induction of thymidine kinase and DNase in varicella-zoster virus-infected cells and kinetic properties of the virus-induced thymidine kinase. Journal of Virology 31:172–177
    [Google Scholar]
  4. Davidson R. L., Adelstein S. J., Oxman M. N. 1973; Herpes simplex virus as a source of thymidine kinase for thymidine kinase-deficient mouse cells: suppression and reactivation of the viral enzyme. Proceedings of the National Academy of Sciences, U. S. A 70:1912–1916
    [Google Scholar]
  5. Dobersen M. J., Jerkofsky M., Greer S. 1976; Enzymatic basis for the selective inhibition of varicella-zoster by 5-halogenated analogues of deoxycytidine. Journal of Virology 20:478–486
    [Google Scholar]
  6. Garfinkel B., McAuslan B. R. 1974; Transformation of cultured mammalian cells by viable herpes simplex virus subtypes 1 and 2. Proceedings of the National Academy of Sciences, U. S. A 71:220–224
    [Google Scholar]
  7. Gilead Z., Jeng Y.-H., Wold W. S. M., Sugawara K., Rho H. M., Harter M. L., Green M. 1976; Immunological identification of two adenovirus 2-induced early proteins possibly involved in cell transformation. Nature, London 264:263–266
    [Google Scholar]
  8. Hackstadt T., Mallavia L. P. 1978; Deoxypyrimidine nucleoside metabolism in varicella-zoster virus-infected cells. Journal of Virology 25:510–517
    [Google Scholar]
  9. Honess R. W., Watson D. H. 1974; Herpes simplex virus-specific polypeptides studied by polyacrylamide gel electrophoresis of immunoprecipitates. Journal of General Virology 22:171–185
    [Google Scholar]
  10. Honess R. W., Watson D. H. 1977; Unity and diversity in the herpes viruses. Journal of General Virology 37:15–37
    [Google Scholar]
  11. Kit S., Jorgensen G. N., Liav A., Zaslavsky V. 1977; Purification of vaccinia virus-induced thymidine kinase activity from [3SS]methionine-labelled cells. Virology 77:661–676
    [Google Scholar]
  12. Kit S., Jorgensen G. N., Dubbs D. R., Trkula D., Zaslavsky V. 1978; Detection of herpes simplex virus thymidine kinase polypeptides in cells labelled with 35S-methionine. Intervirology 9:162–172
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Lamb R. A., Etkind P. R., Choppin P. W. 1978; Evidence for a ninth influenza viral polypeptide. Virology 91:60–78
    [Google Scholar]
  15. Lampson L. A. 1980; Immunoprecipitation of monoclonal antibodies. In Monoclonal Antibodies. Hybridomas: A New Dimension in Biological Analysis pp 395–397 Edited by Kennett R. H., McKeam T. J., Bechtol K. B. New York & London: Plenum Press;
    [Google Scholar]
  16. Munyon W. M., Kraiselburd E., Davis D., Mann J. 1971; Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet irradiated herpes simplex virus. Journal of Virology 7:813–820
    [Google Scholar]
  17. Munyon W., Buchsbaum R., Paletti E., Mann J., Kraiselburd E., Davis D. 1972; Electrophoresis of thymidine kinase activity synthesized by cells transformed by herpes simplex virus. Virology 49:683–689
    [Google Scholar]
  18. Ogino T., Shiman R., Rapp F. 1973; Deoxythymidine kinase from rabbit kidney cells infected with herpes simplex virus types 1 and 2. Intervirology 1:80–95
    [Google Scholar]
  19. Ogino T., Otsuka T., Takahashi M. 1976; Induction of deoxypyrimidine kinase activity in human embryonic lung cells infected with varicella-zoster virus. Journal of Virology 21:1232–1235
    [Google Scholar]
  20. Shapiro A. L., Vinuela E., Maizel V. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  21. Suh M., Kessous A., Poirier N., Simard R. 1980; Immunoprecipitation from hamster embryo cells transformed by herpes simplex virus type 2. Virology 104:303–311
    [Google Scholar]
  22. Thouless M. E., Wildy P. 1975; Deoxypyrimidine kinases of herpes simplex types 1 and 2: comparison of serological and structural properties. Journal of General Virology 26:159–170
    [Google Scholar]
  23. Yamanishi K., Matsunaga Y., Ogino T., Taicahashi M., Takamizawa A. 1980; Virus replication and localization of varicella-zoster virus antigens in human embryonic fibroblast cells infected with cell-free virus. Infection and Imnmunity 28:536–541
    [Google Scholar]
  24. Yamanishi K., Matsunaga Y., Ogino T., Lopetegui P. 1981; Biochemical transformation of mouse cells by varicella-zoster virus. Journal of General Virology 56:421–430
    [Google Scholar]
  25. Yokota T., Shigeta S., Iwabuchi T., Ogata M., Takami S. 1982; Mutant strain of varicella-zoster virus deficient in thymidine kinase-inducing activity. International Symposium on Herpesvirus: Clinical, Pharmacological and Basic Aspects pp 102–105 Edited by Shiota H., Cheng Y. C., Prusoff W. H. Amsterdam, Oxford & Princeton: Excerpta Medica;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-5-1181
Loading
/content/journal/jgv/10.1099/0022-1317-64-5-1181
Loading

Data & Media loading...

Most cited Most Cited RSS feed