1887

Abstract

SUMMARY

Scanning electron microscopy and immunofluorescence techniques show that the attachment of adenovirus type 5 to HeLa cells is followed by a temperature-dependent redistribution of virus particles on the cell surface. Metabolic inhibitors and cytochalasin B, a drug that impairs microfilament function, blocked this redistribution. Transmission electron microscopy studies demonstrated that inhibition of redistribution was paralleled by an inhibition of virus internalization. In further experiments virus and cells were incubated at 4 °C and then treated with adenovirus ‘soluble’ antigens to block unoccupied virus receptors. On warming these preparations to 37 °C, the internalization of attached virions was found to be impaired. It is proposed that energy-dependent redistribution of attached adenovirus particles on the cell surface facilitates entry by bringing virions into contact with sufficient receptors to allow internalization.

Keyword(s): adenovirus , EM , receptors and virus entry
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-5-1091
1983-05-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/5/JV0640051091.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-5-1091&mimeType=html&fmt=ahah

References

  1. Alstiel L. D., Landsberger F. R. 1981; Structural changes in BHK cell plasma membrane caused by the binding of vesicular stomatitis virus. Journal of Virology 39:82–86
    [Google Scholar]
  2. Braun J., Fujiwara K., Pollard T. D., Unanue E. R. 1978; Two distinct mechanisms for redistribution of lymphocyte surface macromolecules. Journal of Cell Biology 79:409–418
    [Google Scholar]
  3. Brown D. T., Burlingham B. T. 1973; Penetration of host cell membranes by adenovirus 2. Journal of Virology 12:386–396
    [Google Scholar]
  4. Chardonnet Y., Dales S. 1970; Early events in the interaction of adenoviruses with HeLa cells. I. Penetration of type 5 and intracellular release of the DNA genome. Virology 40:462–477
    [Google Scholar]
  5. Crowell R. L. 1976; Comparative genetic characteristics of picornavirus-receptor interactions. In Cell Membrane Receptors for Viruses, Antigens and Antibodies, Polypeptide Hormones and Small Molecules pp 179–202 Edited by Beers R. F., Bassett E. G. New York: Raven Press;
    [Google Scholar]
  6. Dales S. 1973; Early events in cell-animal virus interactions. Bacteriological Reviews 37:103–135
    [Google Scholar]
  7. Ginsberg H. S., Pereira H. G., Valentine R. C., Wilcox W. C. 1966; A proposed terminology for the adenovirus antigens and virion morphological subunits. Virology 28:782–783
    [Google Scholar]
  8. Gschwender H. H., Traub P. 1979; Mengovirus-induced capping of virus receptors on the plasma membrane of Ehrlich ascites tumour cells. Journal of General Virology 42:439–442
    [Google Scholar]
  9. Helenius A., Kartenbeck J., Simons K., Fries E. 1980; On the entry of Semliki Forest virus into BHK-21 cells. Journal of Cell Biology 84:404–420
    [Google Scholar]
  10. Hughes R. C., Mautner V. 1973; Interaction of adenovirus with host cell membranes. In Membranemediated Information vol 1: Biochemical Functions pp 104–125 Edited by Kent P. New York: Elsevier;
    [Google Scholar]
  11. Marsh M., Helenius A. 1980; Adsorptive endocytosis of SFV. Journal of Molecular Biology 142:439–454
    [Google Scholar]
  12. Mautner V., Willcox H. N. A. 1974; Adenovirus antigens: a model system in mice for subunit vaccination. Journal of General Virology 25:325–336
    [Google Scholar]
  13. Miranda A. F., Godman G. C., Deitch A. D., Tanenbaub S. W. 1974; Action of cytochalasin D on cells of established lines. I. Early events. Journal of Cell Biology 61:481–500
    [Google Scholar]
  14. Morgan C., Rosenkranz H. S., Mednis B. 1969; Structure and development of viruses as observed in the electron microscope. X. Entry and uncoating of adenovirus. Journal of Virology 4:777–796
    [Google Scholar]
  15. Nicolson G. L. 1974; The interactions of lectins with animal cell surfaces. International Review of Cytology 39:89–190
    [Google Scholar]
  16. Patterson S., Bingham R. W. 1976; Electron microscope observations on the entry of avian infectious bronchitis virus into susceptible cells. Archives of Virology 52:191–200
    [Google Scholar]
  17. Patterson S., Macnaughton M. R. 1981; The distribution of human coronavirus strain 229E on the surface of human diploid cells. Journal of General Virology 53:267–273
    [Google Scholar]
  18. Patterson S., Macnaughton M. R. 1982; Replication of human respiratory coronavirus strain 229E in human macrophages. Journal of General Virology 60:307–314
    [Google Scholar]
  19. Patterson S., Oxford J. S., Dourmashkin R. R. 1979; Studies on the mechanism of influenza virus entry into cells. Journal of General Virology 43:223–229
    [Google Scholar]
  20. Pearse B. M. F., Bretcher M. S. 1981; Membrane recycling by coated vesicles. Annual Review of Biochemistry 50:85–101
    [Google Scholar]
  21. Pereira H. G., Valentine R. C., Russell W. C. 1968; Crystallisation of an adenovirus protein. Nature, London 219:946–947
    [Google Scholar]
  22. Philipson L., Lonberg-Holm K., Pettersson U. 1968; Virus-receptor interaction in an adenovirus system. Journal of Virology 2:1064–1075
    [Google Scholar]
  23. Philipson L., Everitt E., Lonberg-Holm K. 1976; Molecular aspects of virus receptor interaction in the adenovirus system. In Cell Membrane Receptors for Viruses, Antigens and Antibodies, Polypeptide Hormones and Small Molecules pp 203–216 Edited by Beers R. F., Bassett E. G. New York: Raven Press;
    [Google Scholar]
  24. Russell W. C., Valentine R. C., Pereira H. G. 1967; The effect of heat on the anatomy of the adenovirus. Journal of General Virology 1:509–522
    [Google Scholar]
  25. Singer S. J. 1974; Molecular biology of cellular membranes with application to immunology. Advances in Immunology 19:1–66
    [Google Scholar]
  26. Svensson U., Persson R., Everitt E. 1981; Virus-receptor interaction in the adenovirus system. I. Identification of virion attachment proteins of the HeLa cell plasma membrane. Journal of Virology 38:70–81
    [Google Scholar]
  27. Winters W. D., Russell W. C. 1971; Studies on the assembly of adenovirus in vitro. Journal of General Virology 10:181–194
    [Google Scholar]
  28. Yefenof E., Klein G., Jondal M., Oldstone M. B. 1976; Surface markers on human B- and T-lymphocytes. IX. Two color immunofluorescence studies on the association between EBV receptors and complement receptors on the surface of lymphoid cell lines. International Journal of Cancer 17:693–700
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-5-1091
Loading
/content/journal/jgv/10.1099/0022-1317-64-5-1091
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error