1887

Abstract

SUMMARY

Nucleoprotein (NP) intermediates in the assembly pathway of parvovirus LuIII were isolated. These structures consist of replicating viral DNA and of preformed viral capsids into which progeny viral DNA is encapsidated concomitant with synthesis. The NP complexes sediment at between 70S and 100S. They are unstable in CsCl and dissociate in the presence of 1 -NaCl. After fixation with glutaraldehyde, however, they accumulate at a density of 1.37 g/ml in CsCl gradients. Electron microscopy of fixed complexes revealed structures consisting of DNA threads associated with capsid-like particles. A considerable proportion of the DNA labelled during short pulses in such complexes, in a subcellular system derived from infected nuclei, can be effectively chased into 110S virions. The resulting 110S particles apparently have to undergo a sequence of morphogenic events to acquire the physicochemical properties of the mature infectious parvovirus.

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1983-05-01
2024-04-23
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References

  1. Baltimore D., Huang A. S. 1968; Isopycnic separation of subcellular components from poliovirus infected and normal HeLa cells. Science 162:572–574
    [Google Scholar]
  2. Baumgartner I., Kuhn C., Fanning E. 1979; Identification and characterization of fast-sedimenting SV40 nucleoprotein complexes. Virology 96:54–63
    [Google Scholar]
  3. Berns K. I., Hauswirth W. W. 1979; Adeno-associated virus. Advances in Virus Research 25:407–449
    [Google Scholar]
  4. Buller R. M. L., Rose J. A. 1978; Characterization of adeno-associated virus-induced polypeptides in KB cells. Journal of Virology 25:331–338
    [Google Scholar]
  5. Coca-Prados M., Hsu M.-T. 1979; Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virus assembly. Journal of Virology 31:199–208
    [Google Scholar]
  6. Fanning E., Baumgartner I. 1980; Role of fast-sedimenting SV40 nucleoprotein complexes in virus assembly. Virology 102:1–12
    [Google Scholar]
  7. Fernandez-Munoz R., Coca-Prados M., Hsu M.-T. 1979; Intracellular forms of simian virus 40 nucleoprotein complexes. I. Methods of isolation and characterization in CV-1 cells. Journal of Virology 29:612–623
    [Google Scholar]
  8. Gautschi M., Reinhard P. 1978; Synthesis of a mammalian parvovirus in Brij-58-lysed cells. Journal of Virology 27:453–456
    [Google Scholar]
  9. Gautschi M., Siegl G., Kronauer G. 1976; Multiplication of parvovirus LuIII in a synchronized culture system. IV. Association of viral structural polypeptides with the host cell chromatin. Journal of Virology 20:29–38
    [Google Scholar]
  10. Gautschi M., Kronauer G., Siegl G., Reinhard P., Gautschi J. R. 1978; Assembly of parvovirus LuIII in Brij-58 lysed cells. In Replication of Mammalian Parvoviruses pp 459–467 Edited by Ward D. C., Tattersall P. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Jakobovits E., Aloni Y. 1980; Isolation and characterization of various forms of simian virus 40 DNA-protein complexes. Virology 102:107–118
    [Google Scholar]
  12. Laemmli U. R. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Majaniemi I., Tratschin J. D., Siegl G. 1981; A reassessment of the nucleic acid and protein components of parvovirus LuIII. Abstracts, Fifth International Congress of Virology (Strasbourg) p 366
    [Google Scholar]
  14. Marongiu M. E., Pani A., Corrlas M. V., Sau M., La Colla P. 1981; Poliovirus morphogenesis. I. Identification of 80S dissociable particles and evidence for the artifactual production of procapsids. Journal of Virology 39:341–347
    [Google Scholar]
  15. Muller D. E., Siegl G. 1983; Maturation of parvovirus LuIII in a subcellular system. I. Optimal conditions for in vitro synthesis and encapsidation of viral DNA. Journal of General Virology 64:1043–1054
    [Google Scholar]
  16. Myers M. W., Carter B. J. 1980; Assembly of adeno-associated virus. Virology 102:71–82
    [Google Scholar]
  17. Paradiso P. R. 1981; Infectious process of the parvovirus H-l: correlation of protein content, particle density, and viral infectivity. Journal of Virology 39:800–807
    [Google Scholar]
  18. Rhode S. L. 1976; Replication process of the parvovirus H-l. V. Isolation and characterization of temperature-sensitive H-l mutants defective in progeny DNA synthesis. Journal of Virology 17:659–667
    [Google Scholar]
  19. Rhode S. L. 1978a; H-l DNA synthesis. In Replication of Mammalian Parvoviruses pp 279–296 Edited by Ward D. C., Tattersall P. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Rhode S. L. 1978b; Replication process of the parvovirus H-l. X. Isolation of a mutant defective in the replicative form DNA replication. Journal of Virology 25:215–223
    [Google Scholar]
  21. Rueckert R. R. 1976; On the structures and morphogenesis of picornaviruses. In Comprehensive Virology vol 6: pp 131–213 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  22. Seidman M. M., Salzman N. P. 1979; Late replicative intermediates are accumulated during simian virus 40 DNA replication in vivo and in vitro. Journal of Virology 30:600–609
    [Google Scholar]
  23. Siegl G., Gautschi M. 1973; The multiplication of parvovirus LuIII in a synchronized culture system. I. Optimum conditions for virus replication. Archiv für die gesamte Virusforschung 40:105–118
    [Google Scholar]
  24. Siegl G., Gautschi M. 1976; Multiplication of parvovirus LuIII in a synchronized culture system. III. Replication of viral DNA. Journal of Virology 17:841–853
    [Google Scholar]
  25. Singer I. L. 1975; Ultrastructural studies of H-l parvovirus replication. II. Induced changes in the deoxy-ribonucleoprotein and ribonucleoprotein components of human NB cell nuclei. Experimental Cell Research 95:205–217
    [Google Scholar]
  26. Singer I. L., Rhode S. L. 1978; Electron microscopy and cytochemistry of H-l parvovirus intracellular morphogenesis. In Replication of Mammalian Parvoviruses pp 479–505 Edited by Ward D. C., Tattersall P. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Singer I. L., Toolan H. W. 1975; Ultrastructural studies of H-l parvovirus replication. I. Cytopathology produced in NB epithelial cells and hamster embryo fibroblasts. Virology 65:40–54
    [Google Scholar]
  28. Tattersall P., Ward D. C. 1976; Rolling hairpin model for replication of parvovirus and linear chromosomal DNA. Nature, London 263:106–109
    [Google Scholar]
  29. Thoma F., Roller T. 1977; Influence of histone HI on chromatin structure. Cell 12:101–107
    [Google Scholar]
  30. Tibbetts C., Giam G.-Z. 1979; In vitro association of empty adenovirus capsids with double-stranded DNA. Journal of Virology 32:995–1005
    [Google Scholar]
  31. Vollenweider H. J., Sogo J. M., Roller T. 1975; A routine method for protein-free spreading of double- and single-stranded nucleic acid molecules. Proceedings of the National Academy of Sciences, U.S.A 72:83–87
    [Google Scholar]
  32. Ward D. C., Dadachanji D. R. 1978; Replication of minute-virus-of-mice DNA. In Replication of Mammalian Parvoviruses pp 297–313 Edited by Ward D. C., Tattersall P. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
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