1887

Abstract

Summary

Phosphonoacetic acid (PAA) effectively inhibited herpesvirus saimiri (HVS) replication and the onset of virus DNA synthesis. A PAA-resistant (P) mutant of HVS was isolated which plaqued efficiently in the presence of concentrations of PAA sufficient to reduce the growth of wild-type virus to < 0.02% of control values. In contrast, virus growth and DNA synthesis in cells infected with unselected strains of herpesvirus saimiri was highly resistant to concentrations of aphidicolin, an inhibitor of α-type polymerases, which completely inhibited the growth and DNA replication of uninfected cells. An increased level of DNA polymerase activity was induced in cells infected with herpesvirus saimiri. This HVS-induced DNA polymerase was more sensitive to PAA but more resistant to aphidicolin than the uninfected cell activity and could also be distinguished on the basis of its response to ionic strength (40 to 50 m-ammonium sulphate for optimal activity versus 20 n for the uninfected cell activity). Under suitable assay conditions, an increase in the PAA-resistance of the DNA polymerase induced by the HVS(P) mutant was demonstrated. Comparison of the effects of aphidicolin and PAA demonstrated that the former was a much more effective and rapid inhibitor of susceptible cell DNA synthesis . Taken together, these results demonstrate novel properties of a DNA polymerase activity in cells infected with herpesvirus saimiri and suggest that aphidicolin should provide a useful reagent to analyse the functions of this enzyme in productive and non-productive infections with the virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-5-1013
1983-05-01
2019-10-15
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-5-1013
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error