%0 Journal Article %A Zeichhardt, Heinz %A Habermehl, Karl-Otto %A Wetz, Klaus %T A Poliovirus-induced Cytoplasmic Membrane Complex is Exploited by the RNA Polymerase of Superinfecting Mouse Elberfeld (ME) Virus %D 1983 %J Journal of General Virology, %V 64 %N 4 %P 951-955 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-64-4-951 %K picornavirus interference %K guanidine %K ME virus RNA polymerase %K membranes %I Microbiology Society, %X SUMMARY The preexistence of a cytoplasmic membrane complex in HEp-2 cells, induced by poliovirus when inhibited in its reproduction by guanidine, was a prerequisite for accelerated reproduction of superinfecting Mouse Elberfeld (ME) virus. Guanidine-inhibited poliovirus induced a membrane complex of 470S that was successively modified into a faster sedimenting membrane complex (up to 700S) by superinfecting ME virus and exploited for ME virus reproduction. The modified membrane complex was the site for ME virus-specific RNA polymerization characterized by the existence of in vivo and in vitro activity of ME virus RNA polymerase associated with the modified membrane complex. Proof of membrane-bound RNA polymerase and newly synthesized ME virus RNA including replicative intermediate led to the conclusion that superinfecting ME virus exploits the ‘poliovirus/guanidine’-induced complex as the site of action of its replication complex. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-4-951