1887

Abstract

SUMMARY

A field isolate, 3b1a, of the wheat take-all fungus var. , was previously shown to be infected with three serologically unrelated viruses, A, B, and C. It is now shown that virus B can be separated into two distinct strains, designated B1 and B2. All four viruses, A, B1, B2 and C, were faithfully transmitted into conidia. However, six out of eight single ascospore cultures derived from 3b1a single conidial cultures were shown to be virus-free. The remaining two single ascospore cultures each contained only one virus, which appeared to be the same in each culture. This virus was serologically indistinguishable from virus B1, but had dsRNA components of mol. wt. lower than those of virus B1 and had only the smaller of the two capsid polypeptide species of virus B1. No DNA provirus molecules homologous to viruses B1, B2 or C could be detected in two of the virus-free ascospore cultures or in one of the virus-infected ascospore cultures. Very low concentrations of virus particles were detected in hyphal tip isolates of . After prolonged storage and subculturing of these isolates, the concentration of virus particles had increased to the level of the parent culture from which the hyphal tip isolates were derived.

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1983-04-01
2022-08-16
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References

  1. Asher M. J. C. 1981; Pathogenic variation. In Biology and Control of Take-all pp 199–218 Edited by Asher M. J. C., Shipton P. J. London & New York: Academic Press;
    [Google Scholar]
  2. Border D. J., Buck K. W., Chain E. B., Kempson-Jones G. F., Lhoas P., Ratti G. 1972; Viruses of Penicillium and Aspergillus species. Biochemical Journal 127:4P–6P
    [Google Scholar]
  3. Bozarth R. F. 1975; The problem and importance of transmission of mycoviruses using cell-free extracts. Abstracts of the Third International Congress for Virology (Madrid, 1975) 148:
    [Google Scholar]
  4. Buck K. W., Girvan R. F. 1977; Comparison of the biophysical and biochemical properties of Penicillium cyaneo-fulvum virus and Penicillium chrysogenum virus. Journal of General Virology 34:145–154
    [Google Scholar]
  5. Buck K. W., Almond M. R., McFadden J. J. P., Romanos M. A., Rawlinson C. J. 1981a; Properties of thirteen viruses and virus variants obtained from eight isolates of the wheat take-all fungus, Gaeumannomyces graminis var. tritici. Journal of General Virology 53:235–245
    [Google Scholar]
  6. Buck K. W., Romanos M. A., McFadden J. J. P., Rawlinson C. J. 1981a; In vitro transcription of double-stranded RNA by virion-associated RNA polymerases of viruses from Gaeumannomyces graminis. Journal of General Virology 57:157–168
    [Google Scholar]
  7. Burnett J. H. 1975; Genetical aspects of fungal pathogenicity. In Mycogenetics pp 259–287 New York & London: John Wiley & Sons;
    [Google Scholar]
  8. Castanho B., Butler E. E. 1978; Rhizoctonia decline: studies on hypovirulence and potential use in biological control. Phytopathology 68:1511–1514
    [Google Scholar]
  9. Chambers S. C. 1970; Nuclear distribution in vegetative cells of Ophiobolus graminis and other cereal root pathogens. Australian Journal of Biological Sciences 23:1105–1107
    [Google Scholar]
  10. Deacon J. W. 1976; Biology of the Gaeumannomyces graminis Arx and Olivier/Phialophora radicicola Cain complex on roots of the Gramineae. European Plant Protection Organisation Bulletin 6:349–363
    [Google Scholar]
  11. Deacon J. W., Henry C. M. 1978; Studies on virulence of the take-all fungus, Gaeumannomyces graminis, with reference to methodology. Annals of Applied Biology 89:401–409
    [Google Scholar]
  12. Derrick K. S. 1973; Quantitative assay for plant viruses using serologically specific electron microscopy. Virology 56:652–653
    [Google Scholar]
  13. Fraenkel-Conrat H., Singer B., Tsugita A. 1961; Purification of viral RNA by means of bentonite. Virology 14:54–58
    [Google Scholar]
  14. Franklin R. M. 1966; Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proceedings of the National Academy of Sciences, U. S. A 55:1504–1511
    [Google Scholar]
  15. Frick L. J., Lister R. M. 1978; Serotype variability in virus-like particles from Gaeumannomyces graminis. Virology 85:504–517
    [Google Scholar]
  16. Ghabrial S. A. 1980; Effects of fungal viruses on their hosts. Annual Review of Phytopathology 18:441–461
    [Google Scholar]
  17. Hornby D. 1981; Inoculum. In Biology and Control of Take-all pp 271–294 Edited by Asher M. J. C., Shipton P. J. London & New York: Academic Press;
    [Google Scholar]
  18. Lapierre H., Lemaire J.-M., Jouan B., Molin G. 1970; Mise en évidence des particules virales associées à une perte de pathogénicité chez le piétin-échaudage des céréales, Ophiobolus graminis Sacc. Comptes rendus hebdomadaires des séances de l′Académie des sciences D271:1833–1836
    [Google Scholar]
  19. Lemaire J.-M., Lapierre H., Jouan B., Bertrand G. 1970; Découverte de particules virales chez certaines souches d’Ophiobolus graminis, agent du piétin-échaudage des céréales: consequences agronomiques prévisibles. Comptes rendus hebdomadaires des séances de Académie d’Agriculture de France 56:1134–1137
    [Google Scholar]
  20. Lhoas P. 1971; Infection of protoplasts from Pénicillium stoloniferum with double-stranded RNA viruses. Journal of General Virology 13:365–367
    [Google Scholar]
  21. McDonell M. W., Simon M. N., Studier F. W. 1977; Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. Journal of Molecular Biology 110:119–146
    [Google Scholar]
  22. Moffitt E. M., Lister R. M. 1975; Application of a serological screening test for detecting double-stranded RNA mycoviruses. Phytopathology 65:851–859
    [Google Scholar]
  23. Pallett I. H. 1976; Interactions between fungi and their viruses. In Microbial and Plant Protoplasts pp 107–124 Edited by Peberdy J. F., Rose A. H., Rogers H. J., Cocking E. C. London: Academic Press;
    [Google Scholar]
  24. Rawlinson C. J., Buck K. W. 1981; Viruses in Gaeumannomyces and Phialophora spp. In Biology and Control of Take-all pp 151–172 Edited by Asher M. J. C., Shipton P. J. London & New York: Academic Press;
    [Google Scholar]
  25. Rawlinson C. J., Hornby D., Pearson V., Carpenter J. M. 1973; Virus-like particles in the take-all fungus, Gaeumannomyces graminis. Annals of Applied Biology 74:197–209
    [Google Scholar]
  26. Rawlinson C. J., Muthyalu G., Deacon J. W. 1977; Natural transmission of viruses in Gaeumannomyces and Phialophora spp. Abstracts of the Second International Mycological Congress (Tampa, Florida) 558:
    [Google Scholar]
  27. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  28. Szekely M., Sanger F. 1969; Use of polynucleotide kinase in fingerprinting non-radioactive nucleic acids. Journal of Molecular Biology 43:607–617
    [Google Scholar]
  29. Thomas P. S. 1980; Hybridisation of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U. S. A 77:5201–5205
    [Google Scholar]
  30. Timberlake W. E. 1978; Low repetitive DNA content in Aspergillus nidulans. Science 202:973–975
    [Google Scholar]
  31. Tivoli B., Ferault A. C., Lemaire J. M., Spire D. 1979; Aggressivité et particules de type viral dans huit isolats monoascosporés de Gaeumannomyces graminis (Sacc.) Arx et Olivier (Ophiobolus graminis Sacc.). Annales de Phytopathologie 11:259–261
    [Google Scholar]
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