1887

Abstract

SUMMARY

Two host range mutants of avian sarcoma virus (ASV) were isolated from a clone of the Bratislava 77 strain, subgroup C (B77-C). An HR mutant was obtained by adaptation to duck cells and showed almost the same efficiencies of transformation (e.o.t.) and growth in cultures of chick embryo fibroblasts (CEF) and duck embryo fibroblasts (DEF). An HR mutant showed lower e.o.t. and slower growth on DEF than on CEF. Characterization of these mutants and wild-type B77-C showed that (i) unlike the host range mutants reported previously, these mutants and wild-type B77-C have common antigenicity, (ii) the number of infective centres is almost the same for the two mutants and the wild-type, and so the adsorption and integration of these viruses are similar, and (iii) the content of viral RNA in DEF infected with HR is increased and decreased in the case of HR compared with the wild-type.

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1983-04-01
2021-10-15
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References

  1. Altaner C., Temin H. M. 1976; Carcinogenesis by RNA sarcoma viruses. XII. A quantitative study of infection of rat cells in vitro by avian sarcoma viruses. Virology 40:118–134
    [Google Scholar]
  2. Chiswell D. J., Enrietto P. J., Evans S., Quade K., Wyke J. A. 1982; Molecular mechanisms involved in morphological variation of avian sarcoma virus-infected rat cells. Virology 116:428–440
    [Google Scholar]
  3. Deng C. T., Boettiger D., Macpherson I., Yarmus H. E. 1974; The persistence and expression of virus-specific DNA in revertants of Rous sarcoma virus-transformed BHK-21 cells. Virology 62:512–521
    [Google Scholar]
  4. Deng C. T., Stehelin D., Bishop J. M., Varmus H. E. 1977; Characteristics of virus-specific RNA in avian sarcoma virus-transformed BHK-21 cells and revertants. Virology 76:313–330
    [Google Scholar]
  5. Duff R. G., Vogt P. K. 1969; Characteristics of two new avian tumor virus subgroups. Virology 39:18–30
    [Google Scholar]
  6. Graf T., Beug H. 1976; A novel type of cellular variant with altered expression of virus-induced cell transformation. Virology 72:283–286
    [Google Scholar]
  7. Graf T., Friis R. R. 1973; Differential expression of transformation in rat and chicken cells infected with an avian sarcoma virus ts mutant. Virology 56:369–374
    [Google Scholar]
  8. Hayward W. S. 1977; Size and genetic content of viral RNAs in avian oncovirus-infected cells. Journal of Virology 24:47–63
    [Google Scholar]
  9. Krzyzek R. A., Lau A. F., Faras A. J. 1977; Post-transcriptional control of avian oncornavirus transforming gene sequences in mammalian cells. Nature, London 269:175–179
    [Google Scholar]
  10. Krzyzek R. A., Lau A. F., Faras A. J. 1979; Nature of Rous sarcoma virus-specific RNA in transformed and revertant field vole cells. Journal of Virology 29:507–516
    [Google Scholar]
  11. Neiman P. E., Mcmillin-Helsel C., Cooper G. M. 1978; Specific restriction of avian sarcoma viruses by a line of transformed lymphoid cells. Virology 89:360–371
    [Google Scholar]
  12. Quintrell N., Hughes S. H., Varmus H. E., Bishop J. M. 1980; Structure of viral DNA and RNA in mammalian cells infected with avian sarcoma viruses. Journal of Molecular Biology 143:363–393
    [Google Scholar]
  13. Shimakage M. I., Kamahora T., Hakura A., Toyoshima K. 1979; Selective replication of transformation-defective avian sarcoma virus mutants in duck embryo fibroblasts. Journal of General Virology 45:99–105
    [Google Scholar]
  14. Stavnezer E., Ringold G., Varmus H. E., Bishop J. M. 1976; RNA complementary to the genome of RNA tumor viruses in virions and virus producing cells. Journal of Virology 20:342–347
    [Google Scholar]
  15. Stehelin D., Guntaka R. V., Varmus H. E., Bishop J. M. 1976; Purification of DNA complementary tonucleotide sequences required for neoplastic transformation of fibroblasts by avian sarcoma viruses. Journal of Molecular Biology 101:349–365
    [Google Scholar]
  16. Tereba A., Skoog L., Vogt P. K. 1975; RNA tumor virus specific sequences in nuclear DNA of several avian species. Virology 65:524–534
    [Google Scholar]
  17. Varmus H. E., Vogt P. K., Bishop J. M. 1973; Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts. Proceedings of the National Academy of Sciences, U. S. A 70:3067–3071
    [Google Scholar]
  18. Varmus H. E., Guntaka R. V., Fan W. J., Heasley S., Bishop J. M. 1974; Synthesis of viral DNA in the cytoplasm of duck embryo fibroblasts and in enucleated cells after infection by avian sarcoma virus. Proceedings of the National Academy of Sciences, U. S. A 71:3874–3878
    [Google Scholar]
  19. Vogt P. K. 1969; Focusassay of Rous sarcoma virus. in Fundamental Techniques in Virology pp 198–211 Edited by Habel K., Salzman N. P. New York: Academic Press;
    [Google Scholar]
  20. Weiss R. A., Mason W. S., Vogt P. K. 1973; Genetic recombinants and heterozygotes derived from endogenous and exogenous avian RNA tumor viruses. Virology 52:535–552
    [Google Scholar]
  21. Zarling D. A., Temin H. M. 1976; High spontaneous mutation rate of an avian sarcoma virus. Journal of Virology 17:74–84
    [Google Scholar]
  22. Zarling D. A., Mosser A. G., Temin H. M. 1977; Spontaneous mutations affecting the host range of the B77 strain of avian sarcoma virus involve type-specific changes in the virion envelope antigen. Journal of Virology 21:105–112
    [Google Scholar]
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