1887

Abstract

SUMMARY

Eight hybridomas secreting monoclonal antibodies specific to human fibroblast interferon (IFN- ) were identified by a new screening procedure based on immune precipitation with a second antibody and recovery of interferon activity from the immune precipitate. Immunoadsorbents were prepared from these monoclonal antibodies and used for purification of IFN- . Electrophoretically pure IFN- , having a specific activity of 3 × 10 to 6 × 10 units/mg was obtained by a single passage of partially purified IFN- on these immunoadsorbents.

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/content/journal/jgv/10.1099/0022-1317-64-4-905
1983-04-01
2021-10-22
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