1887

Abstract

SUMMARY

Sendai virus grown in LLC-MK2 cells is known to have low infectivity, no detectable haemolysing ability and predominantly uncleaved F glycoprotein. Treatment of such virus with chicken amniotic fluid resulted in a 10- to 10-fold increase in infectivity, the development of haemolysing ability, and cleavage of the F glycoprotein. The ‘Sendai activating enzyme’ (SAE) responsible for these effects required the presence of 1 m-Ca ions for maximum activity. It was inhibited by phenylmethylsulphonyl fluoride and soybean trypsin inhibitor but was unaffected by sulphydryl-blocking agents. The results of gel filtration tests suggested that the molecular weight of SAE was about 55000. SAE may be the natural proteolytic activator of Sendai virus in a soluble form.

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/content/journal/jgv/10.1099/0022-1317-64-4-813
1983-04-01
2024-04-25
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References

  1. Bosch F. X., Orlich M., Klenk H.-D., Rott R. 1979; The structure of the hemagglutinin, a determinant for the pathogenicity of influenza viruses. Virology 95:197–207
    [Google Scholar]
  2. Choppin P. W., Compans R. W. 1975; Reproduction of paramyxoviruses. In Comprehensive Virology vol 4: pp 95–178 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  3. Choppin P. W., Scheid A. 1980; The role of viral glycoproteins in adsorption, penetration and pathogenicity of viruses. Review of Infectious Diseases 2:40–61
    [Google Scholar]
  4. De Martino G. N. 1981; Calcium-dependent proteolytic activity in rat liver: identification of two proteases with different calcium requirements. Archives of Biochemistry and Biophysics 211:253–257
    [Google Scholar]
  5. Gething M. J., White J. M., Waterfield M. D. 1978; Purification of the fusion protein of Sendai virus: analysis of the NH2-terminal sequence generated during precursor activation. Proceedings of the National Academy of Sciences, U. S. A 75:2737–2740
    [Google Scholar]
  6. Homma M., Ohuchi M. 1973; Trypsin action on the growth of Sendai virus in tissue culture cells. III. Structural differences of Sendai viruses grown in eggs and tissue culture cells. Journal of Virology 12:1457–1465
    [Google Scholar]
  7. Homma M., Shimizu K., Shimizu Y. K., Ishida N. 1976; On the study of Sendai virus hemolysis. I. Complete Sendai virus lacking in hemolytic activity. Virology 71:41–47
    [Google Scholar]
  8. Hsu M., Scheid A., Choppin P. W. 1981; Activation of the Sendai virus fusion protein (F) involves a conformational change with exposure of a new hydrophobic region. Journal of Biological Chemistry 256:3557–3563
    [Google Scholar]
  9. Kohama T., Garten W., Klenk H.-D. 1981; Changes in conformation and charge paralleling proteolytic activation of Newcastle disease virus glycoproteins. Virology 111:364–376
    [Google Scholar]
  10. Kubota S., Suzuki K., Imahori K. 1981; A new method for the preparation of a calcium activated neutral protease highly sensitive to calcium ions. Biochemical and Biophysical Research Communications 100:1189–1194
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Laskowski M., Kato I. 1980; Protein inhibitors of proteinases. Annual Review of Biochemistry 49:593–626
    [Google Scholar]
  13. Lazarowitz S. G., Compans R. W., Choppin P. W. 1971; Influenza virus structural and nonstructural proteins in infected cells and their plasma membranes. Virology 46:830–843
    [Google Scholar]
  14. Mountcastle W. E., Compans R. W., Choppin P. W. 1971; Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 7:47–52
    [Google Scholar]
  15. Muramatsu M., Homma M. 1980; Trypsin action on the growth of Sendai virus in tissue culture cells. V. An activating enzyme for Sendai virus in the chorioallantoic fluid of the embryonated chicken egg. Microbiology and Immunology 24:113–122
    [Google Scholar]
  16. Nagai Y., Klenk H.-D., Rott R. 1976; Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508
    [Google Scholar]
  17. Ohuchi M., Homma M. 1976; Trypsin action on the growth of Sendai virus in tissue culture cells. IV. Evidence for activation of Sendai virus by cleavage of a glycoprotein. Journal of Virology 18:1147–1150
    [Google Scholar]
  18. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
  19. Scheid A., Choppin P. W. 1976; Protease activation mutants of Sendai virus. Activation of biological properties by specific proteases. Virology 69:265–277
    [Google Scholar]
  20. Scheid A., Graves M. C., Silver S. M., Choppin P. W. 1978; Studies on the structure and functions of paramyxo-virus glycoproteins. In Negative Strand Viruses and the Host Cell pp 181–193 Edited by Mahy B. W. J., Barry R. D. New York: Academic Press;
    [Google Scholar]
  21. Shibuta H., Akami M., Matumoto M. 1971; Plaque formation by Sendai virus of parainfluenza virus group, type I on monkey, calf kidney and chick embryo cell monolayers. Japanese Journal of Microbiology 15:175–183
    [Google Scholar]
  22. Silver S. M., Scheid A., Choppin P. W. 1978; Loss on serial passage of rhesus monkey kidney cells of proteolytic activity required for Sendai virus activation. Infection and Immunity 20:235–241
    [Google Scholar]
  23. Skehel J. J., Waterfield M. D., Mccauley J. W., Elder K., Wiley D. C. 1980; Studies on the structure of the haemagglutinin. Philosophical Transactions of the Royal Society of London B-288:335–339
    [Google Scholar]
  24. Sugita K., Maru M., Sato K. 1974; A sensitive plaque assay for Sendai virus in an established line of monkey kidney cells. Japanese Journal of Microbiology 18:262–264
    [Google Scholar]
  25. Tozawa H., Watanabe M., Ishida N. 1973; Structural components of Sendai virus. Serological and physicochemical characterization of hemagglutinin subunit associated with neuraminidase activity. Virology 55:242–253
    [Google Scholar]
  26. Ward C. W., Dopheide T. A. 1979; Primary structures of the Hong Kong (H3) haemagglutinin. British Medical Bulletin 35:51–56
    [Google Scholar]
  27. Zhirnov O. P., Ovcharenko A. V., Bukrinskaya A. G. 1982; A modified plaque assay method for accurate analysis of infectivity of influenza viruses with uncleaved hemagglutinin. Archives of Virology 71:177–183
    [Google Scholar]
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