1887

Abstract

SUMMARY

The ability of the compound 2′,3′-dideoxycytidine 5′-triphosphate (ddCTP) to serve as an inhibitor of viral RNA synthesis was examined using an system that supports vesicular stomatitis virus (VSV) protein synthesis, transcription and replication. Viral RNA synthesis was inhibited by 87 and 98% of control, respectively, in reactions containing 1 m- and 10 m-ddCTP in place of CTP. VSV RNA replication and transcription were inhibited equally by ddCTP. At a concentration of 1 m-ddCTP, there was no inhibitory effect on viral protein synthesis; at 10 m-ddCTP, total protein synthesis was inhibited by 30% as compared to control reactions. The presence of ddCTP had no effect on the size or relative molar amounts of each protein synthesized as analysed by electrophoresis on polyacrylamide gels. This is the first report describing a compound that will inhibit VSV RNA synthesis without compromising the concurrent synthesis and modification of proteins.

Keyword(s): ddCTP , RNA synthesis inhibition and VSV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-3-743
1983-03-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/3/JV0640030743.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-3-743&mimeType=html&fmt=ahah

References

  1. Banerjee A. K., Chanda P. K., Talib S. 1981; Mode of transcription and replication of vesicular stomatitis virus genome RNA in vitro. In The Replication of Negative-Strand Viruses pp 759–768 Edited by Bishop D. H. L., Compans R. W. New York: Elsevier/North-Holland;
    [Google Scholar]
  2. Chanda P. K., Banerjee A. K. 1980; Inhibition of vesicular stomatitis virus transcriptase in vitro by phosphonoformate and ara-ATP. Virology 107:562–566
    [Google Scholar]
  3. Davis N. L., Wertz G. W. 1981; Preliminary characterization of a cell-free system for vesicular stomatitis virus negative-strand RNA synthesis. In The Replication of Negative-Strand Viruses pp 789–795 Edited by Bishop D. H. L., Compans R. W. New York: Elsevier/North-Holland;
    [Google Scholar]
  4. Davis N. L., Wertz G. W. 1982; Synthesis of vesicular stomatitis virus negative-strand RNA in vitro: dependence on viral protein synthesis. Journal of Virology 41:821–832
    [Google Scholar]
  5. Kingsford L., Emerson S. U. 1980; Transcriptional activities of different phosphorylated species of NS protein purified from vesicular stomatitis virions and cytoplasm of infected cells. Journal of Virology 33:1097–1105
    [Google Scholar]
  6. Moyer S. A. 1981; Alteration of the 5′-terminal caps of the mRNAs of vesicular stomatitis virus by cycloleucine in vivo. Virology 112:157–168
    [Google Scholar]
  7. Moyer S. A., Holmes K. S. 1979; The specific inhibition of vesicular stomatitis virus replication by toyocamycin. Virology 98:99–107
    [Google Scholar]
  8. Schubert M., Lazzarini R. A. 1982; In vitro transcription of vesicular stomatitis virus: incorporation of deoxyguanosine and deoxycytidine, and formation of deoxyguanosine caps. Journal of Biological Chemistry 257:2968–2973
    [Google Scholar]
  9. Soria M., Little S. P., Huang A. S. 1974; Characterization of vesicular stomatitis virus nucleocapsids. I. Complementary 40S RNA molecules in nucleocapsids. Virology 61:270–280
    [Google Scholar]
  10. Wertz G. W., Davis N. L. 1979; RNase III cleaves vesicular stomatitis virus genome-length RNAs but fails to cleave viral mRNAs. Journal of Virology 30:108–115
    [Google Scholar]
  11. Wertz G. W., Levine M. 1973; Ribonucleic acid synthesis by vesicular stomatitis virus and a small plaque mutant: effect of cycloheximide. Journal of Virology 12:253–264
    [Google Scholar]
  12. Witt D. J., Summers D. F. 1980; Relationship between virion-associated kinase-effected phosphorylation and transcription activity of vesicular stomatitis virus. Virology 107:34–49
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-3-743
Loading
/content/journal/jgv/10.1099/0022-1317-64-3-743
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error