Studies on the SV40-like Papovavirus SV40-GBM. I. Genomic Analysis by Restriction Endonucleases and Electron Microscopy after Propagation in CV-1 Monkey Cells Free

Abstract

SUMMARY

Infection of CV-1 monkey cells with SV40-GBM, a papovavirus isolated from a human glioblastoma multiforme, resulted in the appearance of defective viral DNA molecules. In contrast to SV40 wild-type, two main types of variant DNA molecules could be found after three viral passages at multiplicities of infection of about 10. The molecules of one variant DNA (GBM3-L) were about 19% shorter than the GBM3-H DNA molecules and the DNA of the original GBM isolate, as demonstrated by electron microscopy. Restriction enzyme analysis revealed that GBM3-L DNA had lost both the RI and the II cleavage sites which are located in the late viral genome region. Furthermore, SV40 GBM3-L did not possess the two II sites which are located in the late genome region, and a portion of the GBM3-H and GBM3-L DNA molecules had lost the unique I site. Heteroduplex analysis verified that the rearrangements in the GBM3-L DNA are located only in the late region of this DNA. The possible differences between SV40 wild-type and SV40-GBM are discussed on the basis of these results.

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/content/journal/jgv/10.1099/0022-1317-64-3-733
1983-03-01
2024-03-28
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References

  1. Campo M. S., Cameron I. R., Rogers M. E. 1978; Tandem integration of complete and defective SV40 genomes in mouse-human somatic cell hybrids. Cell 15:1411–1426
    [Google Scholar]
  2. Davis R. W., Simon M., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymology 21:413–428
    [Google Scholar]
  3. Fiori M., Di Mayorca G. 1976; Occurrence of BK virus DNA in DNA obtained from certain human tumours. Proceedings of the National Academy of Sciences of the United States of America 73:4662–4666
    [Google Scholar]
  4. Geissler E., Scherneck S., Theile M., Herold H. J., Staneczek W., Zimmermann W., Krause H., Prokoph H., Vogel F., Platzer M. 1980; Studies on the mutagenic and transforming activities of SV40-like viruses. In Leukaemias, Lymphomas and Papillomas: Comparative Aspects pp 43–55 Edited by Bachmann P. A. London: Taylor & Francis;
    [Google Scholar]
  5. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  6. Krieg P., Amtmann E., Jones D., Fischer H., Zang K., Sauer G. 1981; Episomal Simian virus 40 genomes in human brain tumors. Proceedings of the National Academy of Sciences of the United States of America 78:6446–6450
    [Google Scholar]
  7. Martin M. A., Gelb L. D., Garon C., Takemoto K. K., Lee T. N. H., Sack G. H. Jr, Nathans D. 1974; Characterization of‘heavy’ and ‘light’ SV40-Iike particles from a patient with PML. Virology 59:179–189
    [Google Scholar]
  8. May G., Fischer H., Zang K. D. 1979; SV40-related T-antigen expression in human meningiomas with normal and G22-monosomic karyotype. Journal of General Virology 43:697–700
    [Google Scholar]
  9. Meinke W., Goldstein D., Smith R. 1979; Simian virus 40-related DNA sequences in a human brain tumor. Neurology 29:1590–1594
    [Google Scholar]
  10. Mertz J. E. 1975 In Advanced Techniques in Biological Electron Microscopy 1978 p 136 Edited by Koehler K. Berlin, Heidelberg & New York: Springer-Verlag;
    [Google Scholar]
  11. Mertz J. E., Carbon J., Herzberg M., Davis R. W., Berg P. 1975; Isolation and characterization of individual clones of simian virus 40 mutants containing deletions, duplications and insertions in their DNA. Cold Spring Harbor Symposia on Quantitative Biology 39:69–84
    [Google Scholar]
  12. O’neill F. J., Carroll D. 1978; Appearance of defective simian virus 40 following infection of cultured human glioblastoma cells. Virology 87:109–119
    [Google Scholar]
  13. Scherneck S., Luebbe L., Geissler E., Nisch G., Rudolph M., Waehlte H., Weickmann F., Zimmermann W. 1979a; Detection of simian virus 40 related T-antigen in human meningiomas. Zentralblatt für Neurochirurgie 40:121–130
    [Google Scholar]
  14. Scherneck S., Rudolph M., Geissler E., Vogel L., Luebbe F., Waehlte H., Nisch G., Weickmann F., Zimmermann W. 1979b; Isolation of a SV40-like papovavirus from a human glioblastoma. International Journal of Cancer 24:523–531
    [Google Scholar]
  15. Smith J. W., Pinkel D., Dabrowski S. 1969; Detection of a small virus in a cultivated human WILM’s tumor. Cancer 24:527–531
    [Google Scholar]
  16. Soriano F., Shelburne C. E., Gokcen M. 1974; Simian virus 40 in a human cancer. Nature, London 249:421–424
    [Google Scholar]
  17. Takemoto K. K., Rabson A. S., Mullarkey M. F., Blaese R. N., Garon C. F., Nelson D. 1974; Isolation of papovaviruses from brain tumors and urine of a patient with Wiscott-Aldrich syndrome. Journal of the National Cancer Institute 53:1205–1207
    [Google Scholar]
  18. Weiss A. F., Portmann R., Fischer H., Simon J., Zang K. D. 1975; Simian virus 40 related antigens in three human meningiomas with defined chromosome loss. Proceedings of the National Academy of Sciences of the United States of America 72:609–613
    [Google Scholar]
  19. Zimmermann W., Scherneck S., Geissler E., Nisch G. 1981; Demonstration of SV40-related tumor antigen in human meningiomas by different hamster SV40-T-antisera. Acta virologica 25:199–204
    [Google Scholar]
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