1887

Abstract

SUMMARY

The reduced rate of synthesis, maturation and degradation as well as the level of accumulation of the intracellular virus proteins in VSV-infected cells may account for the overall reduction (less than 10-fold) of progeny virion yield due to interferon (IFN); however, the deficiency of the virions proteins, G and M, which apparently caused a drastic loss of infectivity of these progeny virions (about 1000-fold) cannot be easily explained, because the concentrations of G and M proteins relative to other virus proteins were not reduced in the cell. In fact, intracellular M protein was significantly increased. Moreover, the virus proteins in IFN-treated and control cells were synthesized and accumulated in large excess of the amount incorporated into the released virions. The reduction in the intracellular activity of GlcNac-P-P-Dol transferase did not appear to play a direct role in the antiviral mechanism in this system. Our results, however, do suggest that the deficiency of G and M proteins in the virion is related to specific inhibition of the incorporation of either or both of these proteins in the virus assembly process.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-3-707
1983-03-01
2022-01-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/3/JV0640030707.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-3-707&mimeType=html&fmt=ahah

References

  1. Billiau A., Edy V. G., Sobis H., De somer P. 1974; Influence of interferon on virus particle synthesis in oncornavirus carrier lines. II. Evidence for a direct effect on particle release. International Journal of Cancer 14:335–340
    [Google Scholar]
  2. Billiau A., Edy V. G., Declercq E., Hermans H., Desomer P. 1975; Influence of interferon on the synthesis of virus particles in oncornavirus carrier cell lines. III. Survey of effects on A- B- and C-type oncornaviruses. International Journal of Cancer 15:947–950
    [Google Scholar]
  3. Bishop D. H. L., Repik P., Obijeski I. F., Moore N., Wagner R. R. 1975; Restitution of infectivity to spikeless vesicular stomatitis virus by solubilized viral components. Journal of Virology 16:75–84
    [Google Scholar]
  4. Chang E. H., Mims S. J., Trichie T. J., Friedman R. M. 1977; A morphologic study of interferon inhibition of murine leukaemia virus release: correlation with biochemical data. Journal of General Virology 34:363–367
    [Google Scholar]
  5. Chang E. H., Jay F. T., Friedman R. M. 1978; Physical, morphological and biochemical alterations in the membrane of AKR mouse cells after interferon treatment. Proceedings of the National Academy of Sciences of the United States of America 75:1859–1863
    [Google Scholar]
  6. Friedman R. M., Ramseur J. M. 1974; Inhibition of murine leukaemia virus production in chronically infected AKR cells: a novel effect of interferon. Proceedings of the National Academy of Sciences of the United States of America 17:3542–3544
    [Google Scholar]
  7. Friedman R. M., Chang E. H., Ramseur J. M., Myers M. W. 1975; Interferon-directed inhibition of chronic murine leukemia virus production in cell cultures: lack of effect on intracellular viral markers. Journal of Virology 16:569–574
    [Google Scholar]
  8. Hunt L. A., Etchison J. R., Summers D. T. 1978; Oligosaccharide chains are trimmed during synthesis of the envelope glycoprotein of vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 75:754–758
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 211:680–685
    [Google Scholar]
  10. Laskey R. A., Mills A. D. 1975; Quantitative detection of 3H and 14C in polyacrylamide gel by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  11. Maheshwari R. K., Banerjee D. K., Waechter C. J., Olden K., Friedman R. M. 1980a; Interferon treatment inhibits glycosylation of a viral protein. Nature, London 287:454–456
    [Google Scholar]
  12. Maheshwari R. K., Jay F. T., Friedman R. M. 1980b; Selective inhibition of glycoprotein and membrane protein of vesicular stomatitis virus from interferon-treated cells. Science 207:540–541
    [Google Scholar]
  13. O’Farrell P. J. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  14. Pitha P. M., Rowe W. P., Oxman M. N. 1976; Effect of interferon on exogenous, endogenous and chronic murine leukaemia virus infection. Virology 70:324–338
    [Google Scholar]
  15. Robbins P. W., Hubbard S. C., Turco S. J., Wirth D. F. 1977; Proposal for a common oligosaccharide intermediate in the synthesis of membrane glycoproteins. Cell 12:893–900
    [Google Scholar]
  16. Tabas J., Schlesinger S., Kornfeld S. 1978; Processing of high-mannose oligosaccharides to form complex type oliogosaccharides on the newly synthesized polypeptides of VSV G protein and the IgG heavy chains. Journal of Biological Chemistry 253:716–722
    [Google Scholar]
  17. Wong P. K. Y., Yuen P. H., Macleod R., Chang E. H., Myers M. W., Friedman R. M. 1977; The effect of interferon on de novo infection of Moloney murine leukaemia virus. Cell 10:245–252
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-3-707
Loading
/content/journal/jgv/10.1099/0022-1317-64-3-707
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error