RT Journal Article SR Electronic(1) A1 Crawford, D. H. A1 Callard, R. E. A1 Muggeridge, M. I. A1 Mitchell, D. M. A1 Zanders, E. D. A1 Beverley, P. C. L.YR 1983 T1 Production of Human Monoclonal Antibody to X31 Influenza Virus Nucleoprotein JF Journal of General Virology, VO 64 IS 3 SP 697 OP 700 DO https://doi.org/10.1099/0022-1317-64-3-697 PB Microbiology Society, SN 1465-2099, AB SUMMARY In vitro stimulation of human peripheral blood mononuclear cells with X31 influenza virus antigen has been used to enrich for specific anti-X31 antibody-producing cells. Following Epstein—Barr virus transformation of these stimulated cells, a cell line which produces human antibody to X31 virus was derived and subsequently cloned. The cloned cells secrete an IgG1 κ antibody which is directed against the nucleoprotein of A type influenza virus. Culture supernatants contain 10 to 20 µg/ml of specific antibody which is now used as a standard for the ELISA assay used in our laboratory to detect antibodies to influenza virus., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-3-697