%0 Journal Article %A Crawford, D. H. %A Callard, R. E. %A Muggeridge, M. I. %A Mitchell, D. M. %A Zanders, E. D. %A Beverley, P. C. L. %T Production of Human Monoclonal Antibody to X31 Influenza Virus Nucleoprotein %D 1983 %J Journal of General Virology, %V 64 %N 3 %P 697-700 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-64-3-697 %K human monoclonal antibody %K nucleoprotein %K influenza virus X31 %I Microbiology Society, %X SUMMARY In vitro stimulation of human peripheral blood mononuclear cells with X31 influenza virus antigen has been used to enrich for specific anti-X31 antibody-producing cells. Following Epstein—Barr virus transformation of these stimulated cells, a cell line which produces human antibody to X31 virus was derived and subsequently cloned. The cloned cells secrete an IgG1 κ antibody which is directed against the nucleoprotein of A type influenza virus. Culture supernatants contain 10 to 20 µg/ml of specific antibody which is now used as a standard for the ELISA assay used in our laboratory to detect antibodies to influenza virus. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-3-697