1887

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Volume 64, Issue 3

Characterization of a Defective Form of Tomato Spotted Wilt Virus Free

Abstract

SUMMARY

Defective isolates of tomato spotted wilt virus (TSWV) which produce amorphous masses characteristic of TSWV in infected cells but do not produce enveloped virus particles have been studied. Nucleocapsid protein but no membrane proteins was detected by ELISA in plants infected with defective isolates. The protein of an infectious fraction purified from such plants consisted mainly of nucleocapsid protein. Electron microscope examination of this purified fraction revealed structures resembling the amorphous masses observed in thin sections of leaves infected with the defective isolates. This fraction from the three defective isolates studied contained three RNA segments, the mol. wt. of RNA 1 and RNA 3 being the same as those of normal TSWV particles. The mol. wt. of RNA 2 of the defective isolates was always smaller than that of the RNA 2 of normal TSWV particles. However, their mol. wt. were different for each isolate. Thus, the amorphous masses are aggregates of nucleocapsids of defective TSWV which do not produce normal particles since they have lost the ability to make at least one membrane protein species. This is possibly due to the deletion in RNA 2.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): defective forms , protein composition , RNA deletion and TSWV
© Journal of General Virology 1983
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1983-03-01
2024-04-25
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References

  1. Adam G., Sander E., Shepherd R. J. 1979; Structural differences between pea enation mosaic virus strains affecting transmissibility by Acyrthosiphon pisum (Harris). Virology 92:1–14
     [Google Scholar]
  2. Bailey J. M., Davidson N. 1976; Methylmercury as a reversible denaturing agent for gel electrophoresis. Analytical Biochemistry 70:75–85
     [Google Scholar]
  3. Bol J. F., Brederode F. T., Janze G. C., Rauh D. K. 1975; Studies on sequence homology between the RNAs of alfalfa mosaic virus. Virology 65:1–15
     [Google Scholar]
  4. Choppin P. W., Compans R. W. 1975; Reproduction of paramyxoviruses. Comprehensive Virology 4:95–178
     [Google Scholar]
  5. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
     [Google Scholar]
  6. Clarke R. G., Bath J. E. 1976; Serological properties of aphid- and non aphid-transmissible pea enation mosaic virus isolates. Phytopathology 67:1035–1040
     [Google Scholar]
  7. Davies J. W., Verduin B. J. M. 1979; In vitro synthesis of cowpea chlorotic mottle virus polypeptides. Journal of General Virology 44:545–549
     [Google Scholar]
  8. Goldbach R. W., Borst P., Bollen-De Boer J. E., van Bruggen E. F. J. 1978; The organization of ribosomal RNA genes in the mitochondrial DNA of Tetrahymenapyriformis strain ST. Biochimica et biophysica acta 521:169–186
     [Google Scholar]
  9. Huang A. S., Baltimore D. 1977; Defective interfering animal viruses. Comprehensive Virology 10:73–116
     [Google Scholar]
  10. Hull R. 1976; Particle differences related to aphid-transmissibility of a plant virus. Journal of General Virology 34:183–187
     [Google Scholar]
  11. Ie T. S. 1964; An electron microscope study of tomato spotted wilt virus in the plant cell. Netherlands Journal of Plant Pathology 70:114–115
     [Google Scholar]
  12. Ie T. S. 1971; Electron microscopy of developmental stages of tomato spotted wilt virus in plant cells. Virology 43:468–479
     [Google Scholar]
  13. Ie T. S. 1982; A sap-transmissible, defective form of tomato spotted wilt virus. Journal of General Virology 59:387–391
     [Google Scholar]
  14. Kitajima E. W. 1965; Electron microscopy of viracabeca virus (Brazilian tomato spotted wilt virus) within the host cell. Virology 26:89–99
     [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  16. Milne R. G. 1970; An electron microscope study of tomato spotted wilt virus in sections of infected cells and in negative stain preparations. Journal of General Virology 6:267–276
     [Google Scholar]
  17. Mohamed N. A., Randles J. W., Francki R. I. B. 1973; Protein composition of tomato spotted wilt virus. Virology 56:12–21
     [Google Scholar]
  18. Ralph R. K., Bellamy A. R. 1964; Isolation and purification of undegraded ribonucleic acids. Biochimica et biophysica acta 87:9–16
     [Google Scholar]
  19. Reddy D. V. R., Black L. M. 1977; Isolation and replication of mutant populations of wound tumor virions lacking certain genome segments. Virology 80:336–346
     [Google Scholar]
  20. Reijnders L., Aalbers A. M. J., van Kammen A. 1974; Molecular weights of plant viral RN As determined by gel electrophoresis under denaturing conditions. Virology 60:515–521
     [Google Scholar]
  21. Robbins S. J., Bussell R. H., Rapp F. 1980; Isolation and partial characterization of two forms of cytoplasmic nucleocapsids from measles virus infected cells. Journal of General Virology 47:301–310
     [Google Scholar]
  22. Tas P. W. L., Boerjan M. L., Peters D. 1977a; The structural proteins of tomato spotted wilt virus. Journal of General Virology 36:267–279
     [Google Scholar]
  23. Tas P. W. L., Boerjan M. L., Peters D. 1977b; Purification and serological analysis of tomato spotted wilt virus. Netherlands Journal of Plant Pathology 83:61–72
     [Google Scholar]
  24. Thuring R. W. J., Sanders J. P. M., Borst P. 1975; A freeze-squeeze method for recovering long DNA from agarose gel. Analytical Biochemistry 66:213–220
     [Google Scholar]
  25. Toriyama S., Peters D. 1980; In vitro synthesis of RNA by dissociated lettuce necrotic yellows virus particles. Journal of General Virology 50:125–134
     [Google Scholar]
  26. Verkleij F. N., De Vries P., Peters D. 1982; Evidence that tomato spotted wilt virus RNA is a positive strand. Journal of General Virology 58:329–338
     [Google Scholar]
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Characterization of a Defective Form of Tomato Spotted Wilt Virus
J. Gen. Virol. 64, 677 (1983); https://doi.org/10.1099/0022-1317-64-3-677
/content/journal/jgv/10.1099/0022-1317-64-3-677
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