1887

Abstract

SUMMARY

The nucleotide sequences of the genome RNA species (RNA-1 and RNA-2) of three strains of tobacco rattle virus OR, SYM and CAM, were compared by hybridization with complementary DNA copies. Strain OR was found to contain two RNA-2 species, which differ by about 5 × 10 in mol. wt. (150 nucleotides), but about 90% of whose nucleotide sequences are homologous. The larger of the two contained the genetic information for yellow symptoms in infected spp., whereas the smaller, which probably arose from it by mutation, did not. The RNA-2 species of strains OR, SYM and CAM had no detectable nucleotide sequences in common. RNA-1 of strain OR and of strain SYM were almost identical in sequence, but had no detectable sequence in common with RNA-1 of strain CAM. Only in strain CAM was any nucleotide sequence homology between RNA-1 and RNA-2 detected, amounting to about 450 residues.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-3-657
1983-03-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/3/JV0640030657.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-3-657&mimeType=html&fmt=ahah

References

  1. Bisaro D. M., Siegel A. 1980; A new viral RNA species in tobacco rattle virus-infected tissue. Virology 107:194–201
    [Google Scholar]
  2. Bisaro D. M., Siegel A. 1982; Subgenomic components of tobacco rattle virus in infected tissue. Virology 118:411–418
    [Google Scholar]
  3. Brosius J., Dull T. J., Noller H. F. 1980; Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 77:201–204
    [Google Scholar]
  4. Cooper J. I., Mayo M. A. 1972; Some properties of the particles of three tobravirus isolates. Journal of General Virology 16:285–297
    [Google Scholar]
  5. Darby G., Minson A. C. 1973; The structure of tobacco rattle virus ribonucleic acids: common nucleotide sequences in the RNA species. Journal of General Virology 21:285–295
    [Google Scholar]
  6. Frost R. R., Harrison B. D., Woods R. D. 1967; Apparent symbiotic interaction between particles of tobacco rattle virus. Journal of General Virology 1:57–70
    [Google Scholar]
  7. Harrison B. D., Robinson D. J. 1978; The tobraviruses. Advances in Virus Research 23:25–771
    [Google Scholar]
  8. Harrison B. D., Woods R. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610–620
    [Google Scholar]
  9. Koeppe P., Hamann C. 1980; A program for non-linear regression analysis to be used on desk-top computers. Computer Programs in Biomedicine 12:121–128
    [Google Scholar]
  10. Kurppa A., Jones A. T., Harrison B. D., Bailiss K. W. 1981; Properties of spinach yellow mottle, a distinctive strain of tobacco rattle virus. Annals of Applied Biology 98:243–254
    [Google Scholar]
  11. Lister R. M. 1969; Tobacco rattle, NETU, viruses in relation to functional heterogeneity in plant viruses. Federation Proceedings, Federation of American Societies for Experimental Biology 28:1875–1889
    [Google Scholar]
  12. Lister R. M., Bracker C. E. 1969; Defectiveness and dependence in three related strains of tobacco rattle virus. Virology 37:262–275
    [Google Scholar]
  13. Minson A. C., Darby G. 1973; A study of sequence homology between tobacco rattle virus ribonucleic acids. Journal of General Virology 19:253–262
    [Google Scholar]
  14. Murant A. F., Taylor M. 1978; Estimates of molecular weights of nepovirus RNA species by polyacrylamide gel electrophoresis under denaturing conditions. Journal of General Virology 41:53–61
    [Google Scholar]
  15. Murant A. F., Taylor M., Duncan G. H., Raschké J. H. 1981; Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after denaturation with glyoxal. Journal of General Virology 53:321–332
    [Google Scholar]
  16. Reijnders L., Aalbers A. M. J., Vankammen A., Thuring R. W. J. 1974; Molecular weights of plant viral RNAs determined by gel electrophoresis under denaturing conditions. Virology 60:515–521
    [Google Scholar]
  17. Robinson D. J. 1973; Inactivation and mutagenesis of tobacco rattle virus by nitrous acid. Journal of General Virology 18:215–222
    [Google Scholar]
  18. Robinson D. J., Mayo M. A. 1978; Separation of long and short particles of tobacco rattle virus (TRV). Annual Report, Scottish Horticultural Research Institute 1977:90–91
    [Google Scholar]
  19. Robinson D. J., Barker H., Harrison B. D., Mayo M. A. 1980; Replication of RNA-1 of tomato black ring virus independently of RNA-2. Journal of General Virology 51:317–326
    [Google Scholar]
  20. Sänger H. L. 1968; Characteristics of tobacco rattle virus. I. Evidence that its two particles are functionally defective and mutually complementing. Molecular and General Genetics 101:346–367
    [Google Scholar]
  21. Schwinghamer M. W., Symons R. H. 1977; Translation of the four major RNA species of cucumber mosaic virus in plant and animal cell-free systems and in toad oocytes. Virology 79:88–108
    [Google Scholar]
  22. Wetmur J. G. 1976; Hybridization and renaturation kinetics of nucleic acids. Annual Review of Biophysics and Bioengineering 5:337–361
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-3-657
Loading
/content/journal/jgv/10.1099/0022-1317-64-3-657
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error