Although a mixture of RNA-1 from tomato black ring virus (TBRV) strain A and RNA-2 from TBRV strain G appeared not to infect leaves, it induced the synthesis of TBRV(G) protein when inoculated into tobacco protoplasts. This protein assembled into particles indistinguishable in sedimentation rate and appearance in the electron microscope from top component particles of TBRV(G). Infective RNA-1(A) was synthesized in protoplasts inoculated with RNA-1(A) + RNA-2(G) but was not assembled into nucleoprotein particles. No multiplication of potentially infective RNA-2 was detected, although the synthesis of TBRV(G) protein suggests that at least that part of the RNA-2 coding for coat protein had replicated. Thus, some components of virus particles were produced in these protoplasts but virus multiplication was defective in other respects.
ClarkM. F.,
AdamsA. N.1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
FranssenH.,
GoldbachR.,
BroekhuijsenM.,
MoermanM.,
van KammenA.1982; Expression of middle-component RNA of cowpea mosaic virus: in vitro generation of a precursor to both capsid proteins by a bottom-component RNA-encoded protease from infected cells. Journal of Virology 41:8–17
FritschC.,
MayoM. A.,
MurantA. F.1980; Translation products of genome and satellite RNAs of tomato black ring virus. Journal of General Virology 46:381–389
HarrisonB. D.,
MurantA. F.1977; Nepovirus group. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses No 185
HarrisonB. D.,
MurantA. F.,
MayoM. A.,
RobertsI. M.1974; Distribution of determinants for symptom production, host range and nematode transmissibility between the two RNA components of raspberry ringspot virus. Journal of General Virology 22:233–247
HuberR.,
RezelmanG.,
HibiT.,
van KammenA.1977; Cowpea mosaic virus infection of protoplasts from Samsun tobacco leaves. Journal of General Virology 34:315–323
KuboS.,
HarrisonB. D.,
RobinsonD. I.,
MayoM. A.1975a; Tobacco rattle virus in tobacco mesophyll protoplasts: infection and virus multiplication. Journal of General Virology 27:293–304
KuboS.,
HarrisonB. D.,
BarkerH.1975b; Defined conditions for growth of tobacco plants as sources of protoplasts for virus infection. Journal of General Virology 28:255–257
MauleA. J.,
BoultonM. L.,
EdmundsC.,
WoodK. R.1980; Polyethylene glycol-mediated infection of cucumber protoplasts by cucumber mosaic virus and virus RNA. Journal of General Virology 47:199–203
MayoM. A.,
BarkerH.,
HarrisonB. D.1982; Specificity and properties of the genome-linked proteins of nepoviruses. Journal of General Virology 59:149–162
MurantA. F.1981; Nepoviruses. In Handbook of Plant Virus Infections and Comparative Diagnosis pp 257–332 Edited by
KurstakE.
Ams terdam: Elsevier/North-Holland;
MurantA. F.,
MayoM. A.,
HarrisonB. D.,
GooldR. A.1973; Evidence for two functional RNA species and a ‘satellite’ RNA in tomato black ring virus. Journal of General Virology 19:275–278
MurantA. F.,
TaylorM.,
DuncanG. H.,
RaschkeJ. H.1981; Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after denaturation with glyoxal. Journal of General Virology 53:321–332
RandlesJ. W.,
HarrisonB. D.,
MurantA. F.,
MayoM. A.1977; Packaging and biological activity of the two essential RNA species of tomato black ring virus. Journal of General Virology 36:187–193
RobertsI. M.,
HarrisonB. D.1979; Detection of potato leafroll and potato mop-top viruses by immunosorbent electron microscopy. Annals of Applied Biology 93:289–297
RobinsonD. J.,
BarkerH.,
HarrisonB. D. A.,
MayoM. A.1980; Replication of RNA-1 of tomato black ring virus independently of RNA-2. Journal of General Virology 51:317–326
WimmerE.1979; The genome-linked protein of picomaviruses: discovery, properties and possible functions. In The Molecular Biology of Picomaviruses pp 175–190 Edited by
Peréz-BercoffR.
New York & London: Plenum Press;