Defective Multiplication of a Pseudorecombinant of Tomato Black Ring Virus in Tobacco Protoplasts Free

Abstract

SUMMARY

Although a mixture of RNA-1 from tomato black ring virus (TBRV) strain A and RNA-2 from TBRV strain G appeared not to infect leaves, it induced the synthesis of TBRV(G) protein when inoculated into tobacco protoplasts. This protein assembled into particles indistinguishable in sedimentation rate and appearance in the electron microscope from top component particles of TBRV(G). Infective RNA-1(A) was synthesized in protoplasts inoculated with RNA-1(A) + RNA-2(G) but was not assembled into nucleoprotein particles. No multiplication of potentially infective RNA-2 was detected, although the synthesis of TBRV(G) protein suggests that at least that part of the RNA-2 coding for coat protein had replicated. Thus, some components of virus particles were produced in these protoplasts but virus multiplication was defective in other respects.

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1983-03-01
2024-03-29
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References

  1. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  2. Duncan G. H., Roberts I. M. 1981; Extraction of virus particles from small amounts of material for electron microscope serology. Micron 12:171–173
    [Google Scholar]
  3. Franssen H., Goldbach R., Broekhuijsen M., Moerman M., van Kammen A. 1982; Expression of middle-component RNA of cowpea mosaic virus: in vitro generation of a precursor to both capsid proteins by a bottom-component RNA-encoded protease from infected cells. Journal of Virology 41:8–17
    [Google Scholar]
  4. Fritsch C., Mayo M. A., Murant A. F. 1980; Translation products of genome and satellite RNAs of tomato black ring virus. Journal of General Virology 46:381–389
    [Google Scholar]
  5. Goldbach R., Rezelman G., Van Kammen A. 1980; Independent replication and expression of B-component RNA of cowpea mosaic virus. Nature, London 286:297–300
    [Google Scholar]
  6. Harrison B. D., Barker H. 1978; Protease-sensitive structure needed for infectivity of nepovirus RNA. Journal of General Virology 40:711–715
    [Google Scholar]
  7. Harrison B. D., Murant A. F. 1977; Nepovirus group. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses No 185
    [Google Scholar]
  8. Harrison B. D., Murant A. F., Mayo M. A., Roberts I. M. 1974; Distribution of determinants for symptom production, host range and nematode transmissibility between the two RNA components of raspberry ringspot virus. Journal of General Virology 22:233–247
    [Google Scholar]
  9. Huber R., Rezelman G., Hibi T., van Kammen A. 1977; Cowpea mosaic virus infection of protoplasts from Samsun tobacco leaves. Journal of General Virology 34:315–323
    [Google Scholar]
  10. Kubo S., Harrison B. D., Robinson D. I., Mayo M. A. 1975a; Tobacco rattle virus in tobacco mesophyll protoplasts: infection and virus multiplication. Journal of General Virology 27:293–304
    [Google Scholar]
  11. Kubo S., Harrison B. D., Barker H. 1975b; Defined conditions for growth of tobacco plants as sources of protoplasts for virus infection. Journal of General Virology 28:255–257
    [Google Scholar]
  12. Maule A. J., Boulton M. L., Edmunds C., Wood K. R. 1980; Polyethylene glycol-mediated infection of cucumber protoplasts by cucumber mosaic virus and virus RNA. Journal of General Virology 47:199–203
    [Google Scholar]
  13. Mayo M. A., Barker H., Harrison B. D. 1982; Specificity and properties of the genome-linked proteins of nepoviruses. Journal of General Virology 59:149–162
    [Google Scholar]
  14. Murant A. F. 1981; Nepoviruses. In Handbook of Plant Virus Infections and Comparative Diagnosis pp 257–332 Edited by Kurstak E. Ams terdam: Elsevier/North-Holland;
    [Google Scholar]
  15. Murant A. F., Mayo M. A., Harrison B. D., Goold R. A. 1973; Evidence for two functional RNA species and a ‘satellite’ RNA in tomato black ring virus. Journal of General Virology 19:275–278
    [Google Scholar]
  16. Murant A. F., Taylor M., Duncan G. H., Raschke J. H. 1981; Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after denaturation with glyoxal. Journal of General Virology 53:321–332
    [Google Scholar]
  17. Randles J. W., Harrison B. D., Murant A. F., Mayo M. A. 1977; Packaging and biological activity of the two essential RNA species of tomato black ring virus. Journal of General Virology 36:187–193
    [Google Scholar]
  18. Roberts I. M., Harrison B. D. 1979; Detection of potato leafroll and potato mop-top viruses by immunosorbent electron microscopy. Annals of Applied Biology 93:289–297
    [Google Scholar]
  19. Robinson D. J., Barker H., Harrison B. D. A., Mayo M. A. 1980; Replication of RNA-1 of tomato black ring virus independently of RNA-2. Journal of General Virology 51:317–326
    [Google Scholar]
  20. Wimmer E. 1979; The genome-linked protein of picomaviruses: discovery, properties and possible functions. In The Molecular Biology of Picomaviruses pp 175–190 Edited by Peréz-Bercoff R. New York & London: Plenum Press;
    [Google Scholar]
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